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Schäfer C
,
Ludwig Y
,
Shahin V
,
Kramer A
,
Carl P
,
Schillers H
,
Oberleithner H
.
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Ethanol is the most frequently used drug among humans. We tested the hypothesis whether ethanol, at clinically relevant concentrations modifies, signaling across the nuclear envelope (NE). In cell nuclei isolated from Xenopus oocytes, we measured NE electrical resistance and NE macromolecule permeability 1 to 20 h after addition of ethanol (0.05 to 0.2%). Furthermore, with atomic force microscopy, nuclear pores of the NE were imaged after exposure to ethanol. We found that NE permeability decreased within hours of ethanol exposure. In parallel, nuclei swell and nuclear pores form clusters in the NE. Force measurements on individual nuclear pores indicate that pores found in clusters are stiffer than those found randomly distributed in the NE. Application of a transcription blocker (actinomycin D) or RNase treatment of isolated nuclei in vitro after ethanol exposure prevents the permeability changes. In conclusion, ethanol, at commonly used concentrations, changes NE structure by transcriptional processes in the cell nucleus. Within hours, the NE becomes less permeable for diffusible ions and macromolecules. This could explain altered signaling to and communication with the cell nucleus in the pathophysiology of alcohol abuse.
Albermann,
Evidence for importin alpha independent nuclear translocation of glucocorticoid receptors in Xenopus laevis oocytes.
2004, Pubmed,
Xenbase
Albermann,
Evidence for importin alpha independent nuclear translocation of glucocorticoid receptors in Xenopus laevis oocytes.
2004,
Pubmed
,
Xenbase
Aschner,
Ethanol-induced swelling in neonatal rat primary astrocyte cultures.
2001,
Pubmed
Blow,
A role for the nuclear envelope in controlling DNA replication within the cell cycle.
1988,
Pubmed
,
Xenbase
Brahm,
Permeability of human red cells to a homologous series of aliphatic alcohols. Limitations of the continuous flow-tube method.
1983,
Pubmed
Cronshaw,
Proteomic analysis of the mammalian nuclear pore complex.
2002,
Pubmed
Danker,
Nuclear hourglass technique: an approach that detects electrically open nuclear pores in Xenopus laevis oocyte.
1999,
Pubmed
,
Xenbase
Danker,
Electrophoretic plugging of nuclear pores by using the nuclear hourglass technique.
2001,
Pubmed
,
Xenbase
Denning,
Disorder in the nuclear pore complex: the FG repeat regions of nucleoporins are natively unfolded.
2003,
Pubmed
Enss,
Passive transport of macromolecules through Xenopus laevis nuclear envelope.
2003,
Pubmed
,
Xenbase
Gayer,
Ethanol increases tyrosine hydroxylase gene expression in N1E-115 neuroblastoma cells.
1991,
Pubmed
Goldberg,
Dimples, pores, star-rings, and thin rings on growing nuclear envelopes: evidence for structural intermediates in nuclear pore complex assembly.
1997,
Pubmed
,
Xenbase
Goldberg,
Structural and functional organization of the nuclear envelope.
1995,
Pubmed
,
Xenbase
Görlich,
Transport between the cell nucleus and the cytoplasm.
1999,
Pubmed
Hinshaw,
Architecture and design of the nuclear pore complex.
1992,
Pubmed
,
Xenbase
Jäggi,
Modulation of nuclear pore topology by transport modifiers.
2003,
Pubmed
,
Xenbase
Ludwig,
Hot spot formation in the nuclear envelope of oocytes in response to steroids.
2006,
Pubmed
,
Xenbase
Mattaj,
Nucleocytoplasmic transport: the soluble phase.
1998,
Pubmed
Mazzanti,
Electrical dimension of the nuclear envelope.
2001,
Pubmed
Miles,
Mechanisms of neuronal adaptation to ethanol. Ethanol induces Hsc70 gene transcription in NG108-15 neuroblastoma x glioma cells.
1991,
Pubmed
Oberleithner,
Route of steroid-activated macromolecules through nuclear pores imaged with atomic force microscopy.
2003,
Pubmed
,
Xenbase
Panté,
The nuclear pore complex.
1993,
Pubmed
Peters,
Nucleo-cytoplasmic flux and intracellular mobility in single hepatocytes measured by fluorescence microphotolysis.
1984,
Pubmed
Ribbeck,
Kinetic analysis of translocation through nuclear pore complexes.
2001,
Pubmed
Rout,
The yeast nuclear pore complex: composition, architecture, and transport mechanism.
2000,
Pubmed
Sachinidis,
Early intracellular signalling pathway of ethanol in vascular smooth muscle cells.
1999,
Pubmed
Schäfer,
Aldosterone signaling pathway across the nuclear envelope.
2002,
Pubmed
,
Xenbase
Schneider,
Shape and volume of living aldosterone-sensitive cells imaged with the atomic force microscope.
2004,
Pubmed
Shahin,
Glucocorticoids remodel nuclear envelope structure and permeability.
2005,
Pubmed
,
Xenbase
Shahin,
Evidence for Ca2+- and ATP-sensitive peripheral channels in nuclear pore complexes.
2001,
Pubmed
,
Xenbase
Shulga,
Binding dynamics of structural nucleoporins govern nuclear pore complex permeability and may mediate channel gating.
2003,
Pubmed
Soop,
Nup153 affects entry of messenger and ribosomal ribonucleoproteins into the nuclear basket during export.
2005,
Pubmed
Wünsch,
20-OH-ecdysone swells nuclear volume by alkalinization in salivary glands of Drosophila melanogaster.
1993,
Pubmed
