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Quiescent, full-grown Xenopus oocytes, which are arrested at the G2/M border of meiosis, contain an inactive 42-kDa mitogen-activated protein kinase (p42MAPK) that is activated when oocytes are stimulated to resume the meiotic cell cycle. We have made extracts from these oocytes that respond to four cell cycle activators: oncogenic [Val12]Ras protein, clam cyclins A delta 60 and B delta 97, and the phosphatase inhibitor okadaic acid. All four induce the tyrosine phosphorylation and activation of p42MAPK. Both cyclins and okadaic acid, but not [Val12]Ras, also lead to activation of the endogenous cyclin B/cdc2 kinase complexes in extracts of quiescent oocytes. Using extracts prepared from cycloheximide-arrested interphase cells, we show that although p42MAPK activation can occur in response to cyclin-activated cdc2, the Ras-induced activation of p42MAPK occurs without intervening cdc2 activation. Neither the nononcogenic [Gly12]Ras nor [Val12,Arg186]Ras, a mutant that lacks the C-terminal consensus sequence directing prenylation and subsequent membrane association, is an effective activator of p42MAPK in vitro.
Allende,
Oncogenic ras protein induces meiotic maturation of amphibian oocytes in the presence of protein synthesis inhibitors.
1988, Pubmed,
Xenbase
Allende,
Oncogenic ras protein induces meiotic maturation of amphibian oocytes in the presence of protein synthesis inhibitors.
1988,
Pubmed
,
Xenbase
Barbacid,
ras genes.
1987,
Pubmed
Birchmeier,
ras proteins can induce meiosis in Xenopus oocytes.
1985,
Pubmed
,
Xenbase
Boulton,
Identification of multiple extracellular signal-regulated kinases (ERKs) with antipeptide antibodies.
1991,
Pubmed
Cai,
Effect of a dominant inhibitory Ha-ras mutation on mitogenic signal transduction in NIH 3T3 cells.
1990,
Pubmed
Chao,
Growth factor signaling: where is the specificity?
1992,
Pubmed
Cooper,
Related proteins are phosphorylated at tyrosine in response to mitogenic stimuli and at meiosis.
1989,
Pubmed
,
Xenbase
Crews,
Mouse Erk-1 gene product is a serine/threonine protein kinase that has the potential to phosphorylate tyrosine.
1991,
Pubmed
Der,
Isoprenoid modification and plasma membrane association: critical factors for ras oncogenicity.
1991,
Pubmed
Draetta,
Human cdc2 protein kinase is a major cell-cycle regulated tyrosine kinase substrate.
,
Pubmed
Druker,
Oncogenes, growth factors, and signal transduction.
1989,
Pubmed
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Dunphy,
Fission yeast p13 blocks mitotic activation and tyrosine dephosphorylation of the Xenopus cdc2 protein kinase.
1989,
Pubmed
,
Xenbase
Dunphy,
The cdc25 protein contains an intrinsic phosphatase activity.
1991,
Pubmed
Dunphy,
The Xenopus cdc2 protein is a component of MPF, a cytoplasmic regulator of mitosis.
1988,
Pubmed
,
Xenbase
Fasano,
Sequence and structure of the coding region of the human H-ras-1 gene from T24 bladder carcinoma cells.
1983,
Pubmed
Feig,
Inhibition of NIH 3T3 cell proliferation by a mutant ras protein with preferential affinity for GDP.
1988,
Pubmed
Ferrell,
Cell cycle tyrosine phosphorylation of p34cdc2 and a microtubule-associated protein kinase homolog in Xenopus oocytes and eggs.
1991,
Pubmed
,
Xenbase
Gautier,
cdc25 is a specific tyrosine phosphatase that directly activates p34cdc2.
1991,
Pubmed
,
Xenbase
Gautier,
Purified maturation-promoting factor contains the product of a Xenopus homolog of the fission yeast cell cycle control gene cdc2+.
1988,
Pubmed
,
Xenbase
Gautier,
Dephosphorylation and activation of Xenopus p34cdc2 protein kinase during the cell cycle.
1989,
Pubmed
,
Xenbase
Gotoh,
Xenopus M phase MAP kinase: isolation of its cDNA and activation by MPF.
1991,
Pubmed
,
Xenbase
Gotoh,
In vitro effects on microtubule dynamics of purified Xenopus M phase-activated MAP kinase.
1991,
Pubmed
,
Xenbase
Gould,
Tyrosine phosphorylation of the fission yeast cdc2+ protein kinase regulates entry into mitosis.
1989,
Pubmed
Gross,
Purification and characterization of human H-ras proteins expressed in Escherichia coli.
1985,
Pubmed
Gurdon,
The use of Xenopus oocytes for the expression of cloned genes.
1983,
Pubmed
,
Xenbase
Hagag,
Inhibition of growth factor-induced differentiation of PC12 cells by microinjection of antibody to ras p21.
,
Pubmed
Janknecht,
Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vaccinia virus.
1991,
Pubmed
Jessus,
Tyrosine phosphorylation of p34cdc2 and p42 during meiotic maturation of Xenopus oocyte. Antagonistic action of okadaic acid and 6-DMAP.
1991,
Pubmed
,
Xenbase
Kameshita,
A sensitive method for detection of calmodulin-dependent protein kinase II activity in sodium dodecyl sulfate-polyacrylamide gel.
1989,
Pubmed
Kataoka,
Functional homology of mammalian and yeast RAS genes.
1985,
Pubmed
Kobayashi,
Cyclins and their partners during Xenopus oocyte maturation.
1991,
Pubmed
,
Xenbase
Kremer,
Signal transduction by nerve growth factor and fibroblast growth factor in PC12 cells requires a sequence of src and ras actions.
1991,
Pubmed
Krohne,
Proteins of pore complex--lamina structures from nuclei and nuclear membranes.
1983,
Pubmed
,
Xenbase
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lee,
Regulated expression and phosphorylation of a possible mammalian cell-cycle control protein.
1988,
Pubmed
Leevers,
Activation of extracellular signal-regulated kinase, ERK2, by p21ras oncoprotein.
1992,
Pubmed
Lohka,
Mitotic control by metaphase-promoting factor and cdc proteins.
1989,
Pubmed
Lohka,
Purification of maturation-promoting factor, an intracellular regulator of early mitotic events.
1988,
Pubmed
,
Xenbase
Luca,
Both cyclin A delta 60 and B delta 97 are stable and arrest cells in M-phase, but only cyclin B delta 97 turns on cyclin destruction.
1991,
Pubmed
,
Xenbase
Masui,
Relative roles of the pituitary, follicle cells, and progesterone in the induction of oocyte maturation in Rana pipiens.
1967,
Pubmed
Matsuda,
Xenopus MAP kinase activator: identification and function as a key intermediate in the phosphorylation cascade.
1992,
Pubmed
,
Xenbase
Michaeli,
Mutants of H-ras that interfere with RAS effector function in Saccharomyces cerevisiae.
1989,
Pubmed
Mulcahy,
Requirement for ras proto-oncogene function during serum-stimulated growth of NIH 3T3 cells.
,
Pubmed
Murray,
Cyclin synthesis drives the early embryonic cell cycle.
1989,
Pubmed
,
Xenbase
Pines,
Molecular cloning and characterization of the mRNA for cyclin from sea urchin eggs.
1987,
Pubmed
,
Xenbase
Pondaven,
Activation of M-phase-specific histone H1 kinase by modification of the phosphorylation of its p34cdc2 and cyclin components.
1990,
Pubmed
Porath,
Metal chelate affinity chromatography, a new approach to protein fractionation.
1975,
Pubmed
Posada,
Requirements for phosphorylation of MAP kinase during meiosis in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Posada,
Tyrosine phosphorylation and activation of homologous protein kinases during oocyte maturation and mitogenic activation of fibroblasts.
1991,
Pubmed
,
Xenbase
Robbins,
Evidence for a Ras-dependent extracellular signal-regulated protein kinase (ERK) cascade.
1992,
Pubmed
Roy,
Activation of p34cdc2 kinase by cyclin A.
1991,
Pubmed
,
Xenbase
Ruderman,
Control of the cell cycle in early embryos.
1991,
Pubmed
Saiki,
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.
1988,
Pubmed
Seger,
Microtubule-associated protein 2 kinases, ERK1 and ERK2, undergo autophosphorylation on both tyrosine and threonine residues: implications for their mechanism of activation.
1991,
Pubmed
Shibuya,
Stabilization and enhancement of primary cytostatic factor (CSF) by ATP and NaF in amphibian egg cytosols.
1988,
Pubmed
,
Xenbase
Smith,
Requirement for c-ras proteins during viral oncogene transformation.
,
Pubmed
Solomon,
Cyclin activation of p34cdc2.
1990,
Pubmed
,
Xenbase
Swenson,
The clam embryo protein cyclin A induces entry into M phase and the resumption of meiosis in Xenopus oocytes.
1986,
Pubmed
,
Xenbase
Thomas,
Ras is essential for nerve growth factor- and phorbol ester-induced tyrosine phosphorylation of MAP kinases.
1992,
Pubmed
Toda,
SCH9, a gene of Saccharomyces cerevisiae that encodes a protein distinct from, but functionally and structurally related to, cAMP-dependent protein kinase catalytic subunits.
1988,
Pubmed
Westendorf,
The role of cyclin B in meiosis I.
1989,
Pubmed
,
Xenbase
Willumsen,
The p21 ras C-terminus is required for transformation and membrane association.
,
Pubmed
Wood,
ras mediates nerve growth factor receptor modulation of three signal-transducing protein kinases: MAP kinase, Raf-1, and RSK.
1992,
Pubmed
Wu,
Autophosphorylation in vitro of recombinant 42-kilodalton mitogen-activated protein kinase on tyrosine.
1991,
Pubmed
