Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Atomic force microscopy of cloned nicotinic acetylcholine receptor expressed in Xenopus oocytes.
Lal R
,
Yu L
.
???displayArticle.abstract???
The nicotinic acetylcholine receptor (AChR) was expressed in Xenopus oocytes from in vitro transcribed mRNA and was imaged by atomic force microscopy. A characteristic pentameric structure of AChR was readily observed on the extracellular face of the cell membrane, with a central pore surrounded by protruding AChR subunits. These structures were seen only in mRNA-injected oocytes that also gave acetylcholine-induced membrane currents. The size of individual AChR channels, the angles between subunits, and the interchannel spacing were all compatible with the current model of AChR. In addition, localized patches of microscopic AChR clustering were observed, with packing density approaching that at the neuromuscular junction. These findings show the potential of studying cloned membrane proteins in oocytes for both their surface topography and their structure-function relationship in native membrane without the need for crystallization.
Bertazzon,
Scanning tunneling microscopy imaging of Torpedo acetylcholine receptor.
1992,
Pubmed
Binnig,
Atomic force microscope.
1986,
Pubmed
Bon,
Image analysis of the heavy form of the acetylcholine receptor from Torpedo marmorata.
1984,
Pubmed
Brisson,
Quaternary structure of the acetylcholine receptor.
,
Pubmed
Butt,
Imaging the membrane protein bacteriorhodopsin with the atomic force microscope.
1990,
Pubmed
Clemmer,
Graphite: a mimic for DNA and other biomolecules in scanning tunneling microscope studies.
1991,
Pubmed
Dascal,
The use of Xenopus oocytes for the study of ion channels.
1987,
Pubmed
,
Xenbase
Drake,
Imaging crystals, polymers, and processes in water with the atomic force microscope.
1989,
Pubmed
Edstrom,
Direct visualization of phosphorylase-phosphorylase kinase complexes by scanning tunneling and atomic force microscopy.
1990,
Pubmed
Fertuck,
Quantitation of junctional and extrajunctional acetylcholine receptors by electron microscope autoradiography after 125I-alpha-bungarotoxin binding at mouse neuromuscular junctions.
1976,
Pubmed
Flucher,
Distribution of Na+ channels and ankyrin in neuromuscular junctions is complementary to that of acetylcholine receptors and the 43 kd protein.
1989,
Pubmed
Froehner,
The postsynaptic 43K protein clusters muscle nicotinic acetylcholine receptors in Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Hansma,
Reproducible imaging and dissection of plasmid DNA under liquid with the atomic force microscope.
1992,
Pubmed
Hansma,
Scanning tunneling microscopy and atomic force microscopy: application to biology and technology.
1988,
Pubmed
Henderson,
Actin filament dynamics in living glial cells imaged by atomic force microscopy.
1992,
Pubmed
,
Xenbase
Hoh,
Atomic force microscopy and dissection of gap junctions.
1991,
Pubmed
Holtzman,
Electron microscopy of complexes of isolated acetylcholine receptor, biotinyl-toxin, and avidin.
1982,
Pubmed
Karlin,
The arrangement of the subunits of the acetylcholine receptor of Torpedo californica.
1983,
Pubmed
Kistler,
Structure and function of an acetylcholine receptor.
1982,
Pubmed
Krafte,
Expression of functional sodium channels in stage II-III Xenopus oocytes.
1989,
Pubmed
,
Xenbase
Mitra,
Three-dimensional structure of the nicotinic acetylcholine receptor and location of the major associated 43-kD cytoskeletal protein, determined at 22 A by low dose electron microscopy and x-ray diffraction to 12.5 A.
1989,
Pubmed
Radmacher,
From molecules to cells: imaging soft samples with the atomic force microscope.
1992,
Pubmed
Unwin,
Arrangement of the acetylcholine receptor subunits in the resting and desensitized states, determined by cryoelectron microscopy of crystallized Torpedo postsynaptic membranes.
1988,
Pubmed
Weisenhorn,
Immobilized proteins in buffer imaged at molecular resolution by atomic force microscopy.
1990,
Pubmed
Yang,
New approach for atomic force microscopy of membrane proteins. The imaging of cholera toxin.
1993,
Pubmed
Yoshii,
Equilibrium properties of mouse-Torpedo acetylcholine receptor hybrids expressed in Xenopus oocytes.
1987,
Pubmed
,
Xenbase
Yu,
Single-channel properties of mouse-Torpedo acetylcholine receptor hybrids expressed in Xenopus oocytes.
1991,
Pubmed
,
Xenbase
