Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
A synthetic analog of an abasic site in DNA is efficiently repaired by a short-patch repair mechanism in soluble extracts of Xenopus laevis oocytes (Y. Matsumoto and D. F. Bogenhagen, Mol. Cell. Biol. 9:3750-3757, 1989). We present a detailed analysis of the repair mechanism, using extracts depleted of endogenous nucleotide pools. ATP was required for repair with a sharp optimal concentration of 5 mM. The initial rate of repair was increased by preincubation of the DNA in the extract in the presence of ATP. During this preincubation, the DNA was cleaved on the 5' side of the lesion by a class II apurinic-apyrimidinic endonuclease, but removal of the abasic sugar residue was not observed prior to addition of deoxynucleotides to the reaction. Immediately following DNA synthesis, excision and ligation proceeded in a coordinated manner to complete repair. DNA preincubated in the extract in the absence of deoxynucleotides remained associated with repair enzymes during gel filtration. These observations suggest that the enzymes involved in concerted repair of the abasic site form a complex on DNA.
Glikin,
Chromatin assembly in Xenopus oocytes: in vitro studies.
1984, Pubmed,
Xenbase
Glikin,
Chromatin assembly in Xenopus oocytes: in vitro studies.
1984,
Pubmed
,
Xenbase
Hansson,
Complementation of the xeroderma pigmentosum DNA repair synthesis defect with Escherichia coli UvrABC proteins in a cell-free system.
1990,
Pubmed
Hansson,
Localization of DNA repair synthesis by human cell extracts to a short region at the site of a lesion.
1989,
Pubmed
Labarca,
A simple, rapid, and sensitive DNA assay procedure.
1980,
Pubmed
Lindahl,
Rate of depurination of native deoxyribonucleic acid.
1972,
Pubmed
Loeb,
Apurinic sites as mutagenic intermediates.
1985,
Pubmed
Matsumoto,
Repair of a synthetic abasic site in DNA in a Xenopus laevis oocyte extract.
1989,
Pubmed
,
Xenbase
Randahl,
DNA-repair reactions by purified HeLa DNA polymerases and exonucleases.
1988,
Pubmed
Stanssens,
Efficient oligonucleotide-directed construction of mutations in expression vectors by the gapped duplex DNA method using alternating selectable markers.
1989,
Pubmed
Takeshita,
Oligodeoxynucleotides containing synthetic abasic sites. Model substrates for DNA polymerases and apurinic/apyrimidinic endonucleases.
1987,
Pubmed
Thomas,
Heteroduplex repair in extracts of human HeLa cells.
1991,
Pubmed
Varlet,
DNA mismatch repair in Xenopus egg extracts: repair efficiency and DNA repair synthesis for all single base-pair mismatches.
1990,
Pubmed
,
Xenbase
Wiebauer,
Mismatch-specific thymine DNA glycosylase and DNA polymerase beta mediate the correction of G.T mispairs in nuclear extracts from human cells.
1990,
Pubmed
Wood,
Complementation of the xeroderma pigmentosum DNA repair defect in cell-free extracts.
1988,
Pubmed
