Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Biophys J
1992 Nov 01;635:1406-11. doi: 10.1016/S0006-3495(92)81703-4.
Show Gene links
Show Anatomy links
Tandem linkage of Shaker K+ channel subunits does not ensure the stoichiometry of expressed channels.
McCormack K
,
Lin L
,
Iverson LE
,
Tanouye MA
,
Sigworth FJ
.
???displayArticle.abstract???
Shaker K+ channels are multimeric, probably tetrameric proteins. Substitution of a conserved leucine residue to valine (V2) at position 370 in the Drosophila Shaker 29-4 sequence results in large alterations in the voltage dependence of gating in the expressed channels. In order to determine the effects of this mutation in hybrid channels with a fixed stoichiometry of V2 and wild-type (WT) subunits we generated cDNA constructs of two linked-monomeric subunits similar to the tandem constructs previously reported by Isacoff, E. Y., Y. N. Jan, and L. Y. Jan. (1990. Nature (Lond.). 345:530-534). In addition, we constructed a tandem cDNA containing a wild-type subunit and a truncated nonfunctional subunit (Sh102) that suppresses channel expression. We report that the voltage-dependence of the channels produced with WT and V2 subunits varied significantly with the order of the subunits in the construct (WT-V2 or V2-WT), while the WT-Sh102 construct yielded currents that were much larger than expected. These results suggest that the tandem linkage of Shaker subunits does not guarantee the stoichiometry of the expressed channel proteins.
Catterall,
Structure and function of voltage-sensitive ion channels.
1988, Pubmed
Catterall,
Structure and function of voltage-sensitive ion channels.
1988,
Pubmed
Christie,
Heteropolymeric potassium channels expressed in Xenopus oocytes from cloned subunits.
1990,
Pubmed
,
Xenbase
Gautam,
Alteration of potassium channel gating: molecular analysis of the Drosophila Sh5 mutation.
1990,
Pubmed
,
Xenbase
Gisselmann,
The interference of truncated with normal potassium channel subunits leads to abnormal behaviour in transgenic Drosophila melanogaster.
1989,
Pubmed
Hartmann,
Exchange of conduction pathways between two related K+ channels.
1991,
Pubmed
,
Xenbase
Heginbotham,
The aromatic binding site for tetraethylammonium ion on potassium channels.
1992,
Pubmed
Hoshi,
Biophysical and molecular mechanisms of Shaker potassium channel inactivation.
1990,
Pubmed
,
Xenbase
Hurtley,
Protein oligomerization in the endoplasmic reticulum.
1989,
Pubmed
Isacoff,
Evidence for the formation of heteromultimeric potassium channels in Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Iverson,
The role of the divergent amino and carboxyl domains on the inactivation properties of potassium channels derived from the Shaker gene of Drosophila.
1990,
Pubmed
,
Xenbase
Kamb,
Multiple products of the Drosophila Shaker gene may contribute to potassium channel diversity.
1988,
Pubmed
Kavanaugh,
Multiple subunits of a voltage-dependent potassium channel contribute to the binding site for tetraethylammonium.
1992,
Pubmed
,
Xenbase
Klausner,
Protein degradation in the endoplasmic reticulum.
1990,
Pubmed
MacKinnon,
Determination of the subunit stoichiometry of a voltage-activated potassium channel.
1991,
Pubmed
,
Xenbase
McCormack,
Shaker K+ channel subunits from heteromultimeric channels with novel functional properties.
1990,
Pubmed
,
Xenbase
McCormack,
A role for hydrophobic residues in the voltage-dependent gating of Shaker K+ channels.
1991,
Pubmed
,
Xenbase
Richter,
Differential capacity for translation and lack of competition between mRNAs that segregate to free and membrane-bound polysomes.
1981,
Pubmed
,
Xenbase
Ruppersberg,
Heteromultimeric channels formed by rat brain potassium-channel proteins.
1990,
Pubmed
,
Xenbase
Schoppa,
The size of gating charge in wild-type and mutant Shaker potassium channels.
1992,
Pubmed
Soreq,
The biosynthesis of biologically active proteins in mRNA-microinjected Xenopus oocytes.
1985,
Pubmed
,
Xenbase
Timpe,
Four cDNA clones from the Shaker locus of Drosophila induce kinetically distinct A-type potassium currents in Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Verrall,
The N-terminal domains of acetylcholine receptor subunits contain recognition signals for the initial steps of receptor assembly.
1992,
Pubmed
Yellen,
Mutations affecting internal TEA blockade identify the probable pore-forming region of a K+ channel.
1991,
Pubmed
Yool,
Alteration of ionic selectivity of a K+ channel by mutation of the H5 region.
1991,
Pubmed
,
Xenbase
