Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Biol
1994 May 01;145:3006-12. doi: 10.1128/mcb.14.5.3006-3012.1994.
Show Gene links
Show Anatomy links
Direct activation of phospholipase C-gamma by fibroblast growth factor receptor is not required for mesoderm induction in Xenopus animal caps.
Muslin AJ
,
Peters KG
,
Williams LT
.
???displayArticle.abstract???
Members of the fibroblast growth factor (FGF) family induce mesoderm formation in explants of Xenopus embryonic ectoderm (animal caps). Recent studies have been directed at determining signaling pathways downstream of the FGF receptor that are important in mesoderm induction. We have recently shown that a point mutation in the FGF receptor changing tyrosine 766 to phenylalanine (Y/F mutation) abolishes phospholipase C-gamma (PLC-gamma) activation in mammalian cells. To explore the role of PLC-gamma activation in FGF-stimulated mesoderm induction, we constructed two chimeric receptors, each consisting of the extracellular portion of the platelet-derived growth factor beta receptor, with one having the transmembrane and intracellular portions of the wild-type FGF receptor 1 (PR-FR wt) and the other having the corresponding region of the Y/F766 mutant FGF receptor 1 (PR-FR Y/F766). When expressed in Xenopus oocytes, only PR-FR wt was able to mediate PLC gamma phosphorylation, inositol-1,4,5-trisphosphate accumulation, and calcium efflux in response to platelet-derived growth factor stimulation. However, both receptors mediated mesoderm induction in Xenopus animal caps as measured by cap elongation, muscle-specific actin mRNA induction, and skeletal muscle formation. These results demonstrate that PLC gamma activation by the FGF receptor is not required for FGF-stimulated mesoderm induction.
Amaya,
Expression of a dominant negative mutant of the FGF receptor disrupts mesoderm formation in Xenopus embryos.
1991, Pubmed,
Xenbase
Amaya,
Expression of a dominant negative mutant of the FGF receptor disrupts mesoderm formation in Xenopus embryos.
1991,
Pubmed
,
Xenbase
Berridge,
Inositol trisphosphate and diacylglycerol: two interacting second messengers.
1987,
Pubmed
Berridge,
Neural and developmental actions of lithium: a unifying hypothesis.
1989,
Pubmed
Berridge,
Inositol trisphosphate and calcium signalling.
1993,
Pubmed
Burgess,
Characterization and cDNA cloning of phospholipase C-gamma, a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
1990,
Pubmed
Busa,
Lithium-induced teratogenesis in frog embryos prevented by a polyphosphoinositide cycle intermediate or a diacylglycerol analog.
1989,
Pubmed
,
Xenbase
Cho,
Molecular nature of Spemann's organizer: the role of the Xenopus homeobox gene goosecoid.
1991,
Pubmed
,
Xenbase
Cooke,
Potentiation by the lithium ion of morphogenetic responses to a Xenopus inducing factor.
1989,
Pubmed
,
Xenbase
Downing,
Phospholipase C-gamma, a substrate for PDGF receptor kinase, is not phosphorylated on tyrosine during the mitogenic response to CSF-1.
1989,
Pubmed
Goldschmidt-Clermont,
Regulation of phospholipase C-gamma 1 by profilin and tyrosine phosphorylation.
1991,
Pubmed
Green,
Responses of embryonic Xenopus cells to activin and FGF are separated by multiple dose thresholds and correspond to distinct axes of the mesoderm.
1992,
Pubmed
,
Xenbase
Hemmati-Brivanlou,
A truncated activin receptor inhibits mesoderm induction and formation of axial structures in Xenopus embryos.
1992,
Pubmed
,
Xenbase
Jessell,
Diffusible factors in vertebrate embryonic induction.
1992,
Pubmed
Johnson,
Diverse forms of a receptor for acidic and basic fibroblast growth factors.
1990,
Pubmed
,
Xenbase
Kao,
Dorsalization of mesoderm induction by lithium.
1989,
Pubmed
,
Xenbase
Kao,
The entire mesodermal mantle behaves as Spemann's organizer in dorsoanterior enhanced Xenopus laevis embryos.
1988,
Pubmed
,
Xenbase
Keating,
Platelet-derived growth factor receptor inducibility is acquired immediately after translation and does not require glycosylation.
1989,
Pubmed
Kim,
PDGF stimulation of inositol phospholipid hydrolysis requires PLC-gamma 1 phosphorylation on tyrosine residues 783 and 1254.
1991,
Pubmed
Kintner,
Monoclonal antibodies identify blastemal cells derived from dedifferentiating limb regeneration.
,
Pubmed
,
Xenbase
Krieg,
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
1984,
Pubmed
,
Xenbase
MacNicol,
Raf-1 kinase is essential for early Xenopus development and mediates the induction of mesoderm by FGF.
1993,
Pubmed
,
Xenbase
Maslanski,
Lithium-sensitive production of inositol phosphates during amphibian embryonic mesoderm induction.
1992,
Pubmed
,
Xenbase
Meisenhelder,
Phospholipase C-gamma is a substrate for the PDGF and EGF receptor protein-tyrosine kinases in vivo and in vitro.
1989,
Pubmed
Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed
Mohammadi,
A tyrosine-phosphorylated carboxy-terminal peptide of the fibroblast growth factor receptor (Flg) is a binding site for the SH2 domain of phospholipase C-gamma 1.
1991,
Pubmed
Mohammadi,
Point mutation in FGF receptor eliminates phosphatidylinositol hydrolysis without affecting mitogenesis.
1992,
Pubmed
Morrison,
Platelet-derived growth factor (PDGF)-dependent association of phospholipase C-gamma with the PDGF receptor signaling complex.
1990,
Pubmed
Nishibe,
Increase of the catalytic activity of phospholipase C-gamma 1 by tyrosine phosphorylation.
1990,
Pubmed
Peters,
Point mutation of an FGF receptor abolishes phosphatidylinositol turnover and Ca2+ flux but not mitogenesis.
1992,
Pubmed
Slack,
Inductive effects of fibroblast growth factor and lithium ion on Xenopus blastula ectoderm.
1988,
Pubmed
,
Xenbase
Slack,
Mesoderm induction in early Xenopus embryos by heparin-binding growth factors.
,
Pubmed
,
Xenbase
Smith,
Identification of a potent Xenopus mesoderm-inducing factor as a homologue of activin A.
1990,
Pubmed
,
Xenbase
Thomsen,
Activins are expressed early in Xenopus embryogenesis and can induce axial mesoderm and anterior structures.
1990,
Pubmed
,
Xenbase
Ueno,
A truncated form of fibroblast growth factor receptor 1 inhibits signal transduction by multiple types of fibroblast growth factor receptor.
1992,
Pubmed
,
Xenbase
Wahl,
Platelet-derived growth factor induces rapid and sustained tyrosine phosphorylation of phospholipase C-gamma in quiescent BALB/c 3T3 cells.
1989,
Pubmed
Whitman,
Involvement of p21ras in Xenopus mesoderm induction.
1992,
Pubmed
,
Xenbase
