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Diffusion and formation of microtubule asters: physical processes versus biochemical regulation.
Dogterom M
,
Maggs AC
,
Leibler S
.
???displayArticle.abstract??? Microtubule asters forming the mitotic spindle are assembled around two centrosomes through the process of dynamic instability in which microtubules alternate between growing and shrinking states. By modifying the dynamics of this assembly process, cell cycle enzymes, such as cdc2 cyclin kinases, regulate length distributions in the asters. It is believed that the same enzymes control the number of assembled microtubules by changing the "nucleating activity" of the centrosomes. Here we show that assembly of microtubule asters may be strongly altered by effects connected with diffusion of tubulin monomers. Theoretical analysis of a simple model describing assembly of microtubule asters clearly shows the existence of a region surrounding the centrosome depleted in GTP tubulin. The number of assembled microtubules may in some cases be limited by this depletion effect rather than by the number of available nucleation sites on the centrosome.
Bayley,
Why microtubules grow and shrink.
1993, Pubmed
Bayley,
Why microtubules grow and shrink.
1993,
Pubmed
Belmont,
Real-time visualization of cell cycle-dependent changes in microtubule dynamics in cytoplasmic extracts.
1990,
Pubmed
,
Xenbase
Bré,
Effects of brain microtubule-associated proteins on microtubule dynamics and the nucleating activity of centrosomes.
1990,
Pubmed
Buendia,
Regulation of the microtubule nucleating activity of centrosomes in Xenopus egg extracts: role of cyclin A-associated protein kinase.
1992,
Pubmed
,
Xenbase
Dogterom,
Physical aspects of the growth and regulation of microtubule structures.
1993,
Pubmed
Drechsel,
Modulation of the dynamic instability of tubulin assembly by the microtubule-associated protein tau.
1992,
Pubmed
Joshi,
Gamma-tubulin is a centrosomal protein required for cell cycle-dependent microtubule nucleation.
1992,
Pubmed
Kalt,
Molecular components of the centrosome.
1993,
Pubmed
Kellogg,
Studies on the centrosome and cytoplasmic organization in the early Drosophila embryo.
1991,
Pubmed
Masuda,
In vitro microtubule-nucleating activity of spindle pole bodies in fission yeast Schizosaccharomyces pombe: cell cycle-dependent activation in xenopus cell-free extracts.
1992,
Pubmed
,
Xenbase
Melki,
Oscillations in microtubule polymerization: the rate of GTP regeneration on tubulin controls the period.
1988,
Pubmed
Mitchison,
Tubulin flux in the mitotic spindle: where does it come from, where is it going?
1990,
Pubmed
Mitchison,
Dynamic instability of microtubule growth.
,
Pubmed
Mitchison,
Microtubule assembly nucleated by isolated centrosomes.
,
Pubmed
Moudjou,
A human centrosomal protein is immunologically related to basal body-associated proteins from lower eucaryotes and is involved in the nucleation of microtubules.
1991,
Pubmed
Nigg,
Cellular substrates of p34(cdc2) and its companion cyclin-dependent kinases.
1993,
Pubmed
Oakley,
Gamma-tubulin: the microtubule organizer?
1992,
Pubmed
Ohta,
Microtubule nucleating activity of centrosomes in cell-free extracts from Xenopus eggs: involvement of phosphorylation and accumulation of pericentriolar material.
1993,
Pubmed
,
Xenbase
Raff,
Drosophila gamma-tubulin is part of a complex containing two previously identified centrosomal MAPs.
1993,
Pubmed
Salmon,
Diffusion coefficient of fluorescein-labeled tubulin in the cytoplasm of embryonic cells of a sea urchin: video image analysis of fluorescence redistribution after photobleaching.
1984,
Pubmed
Verde,
Regulation of microtubule dynamics by cdc2 protein kinase in cell-free extracts of Xenopus eggs.
1990,
Pubmed
,
Xenbase
Verde,
Control of microtubule dynamics and length by cyclin A- and cyclin B-dependent kinases in Xenopus egg extracts.
1992,
Pubmed
,
Xenbase
Walker,
Dynamic instability of individual microtubules analyzed by video light microscopy: rate constants and transition frequencies.
1988,
Pubmed
Walker,
Dilution of individual microtubules observed in real time in vitro: evidence that cap size is small and independent of elongation rate.
1991,
Pubmed
