Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Analysis of cell-growth-phase-related variations in hyaluronate synthase activity of isolated plasma-membrane fractions of cultured human skin fibroblasts.
Mian N
.
???displayArticle.abstract???
Hyaluronate synthase activity is localized exclusively in plasma-membrane fractions of cultured human skin fibroblasts. The enzyme activity of plasma membranes prepared from exponential-growth-phase cells was about 6.5 times that of stationary-growth-phase cells. Hyaluronate synthase from exponential-growth-phase cells exhibited lower Km and higher Vmax. values for both UDP-N-acetylglucosamine and UDP-glucuronic acid and higher rate of elongation of hyaluronate chains compared with the enzyme from stationary-growth-phase cells. Hyaluronate synthase exhibited an extremely short half-life, 2.2 h and 3.8 h respectively when cells were treated with cycloheximide and actinomycin D. The cell-growth-phase-dependent variations in hyaluronate synthase activity appear to be due to its high turnover rate as well as due to some post-translational modification of the enzyme protein as cells progress from early exponential to stationary growth phase. The isolated plasma membranes contained a protein (Mr approx. 450,000) that was selectively autophosphorylated from [gamma-32P]ATP in vitro in the presence of hyaluronate precursors in the reaction mixture and that also exhibited some hyaluronate-synthesis-related properties. The 32P-labelled protein isolated from plasma membranes of exponentially growing cells expressed an efficient UDP-[14C]glucuronic acid- and UDP-N-acetyl[3H]glucosamine-binding activity and was able to synthesize oligosaccharides (Mr 5000) of [14C]glucuronic acid and N-acetyl[3H]glucosamine residues. The corresponding protein of stationary-growth-phase cells, which expressed much higher nucleotide-sugar-precursor-binding activity, appeared to have lost its oligosaccharide-synthesizing activity.
Appel,
Cell-free synthesis of hyaluronic acid in Marfan syndrome.
1979, Pubmed
Appel,
Cell-free synthesis of hyaluronic acid in Marfan syndrome.
1979,
Pubmed
Bergeron,
Golgi fractions prepared from rat liver homogenates. II. Biochemical characterization.
1973,
Pubmed
Branton,
Protein phosphorylation and the proliferation of chick embryo fibroblasts: analysis by in vitro phosphorylation using isolated plasma membranes and whole cell homogenates.
1980,
Pubmed
BURTON,
The relation between the synthesis of deoxyribonucleic acid and the synthesis of protein in the multiplication of bacteriophage T2.
1955,
Pubmed
Danielsen,
Biosynthesis of microvillar proteins.
1984,
Pubmed
DAVIDSON,
Heritability and control of differentiated function in cultured cells.
1963,
Pubmed
DAVIDSON,
Gene expression in differentiated cells.
1963,
Pubmed
Davies,
Phosphorylation of filamin and other proteins in cultured fibroblasts.
1977,
Pubmed
EARL,
THE ISOLATION AND PROPERTIES OF CARDIAC RIBOSOMES AND POLYSOMES.
1965,
Pubmed
GIANETTO,
Tissue fractionation studies. 4. Comparative study of the binding of acid phosphatase, beta-glucuronidase and cathepsin by rat-liver particles.
1955,
Pubmed
Hopwood,
Glycosaminoglycan synthesis by cultured human skin fibroblasts after transformation with simian virus 40.
1977,
Pubmed
Hronowski,
The effect of cell density on net rates of glycosaminoglycan synthesis and secretion by cultured rat fibroblasts.
1980,
Pubmed
Ipata,
Sheep brain 5'-nucleotidase. Some enzymic properties and allosteric inhibition by nucleoside triphosphates.
1968,
Pubmed
Joos,
Characterization of specific differences in protein phosphorylation of the plasma membrane and the endoplasmic reticulum of mouse fibroblasts.
1979,
Pubmed
Kittlick,
Acid mucopolysaccharides in fibroblast cultures. 1. Influence of cell density, pH-value and lactate concentration on the MPS distribution pattern.
1976,
Pubmed
Kittlick,
Hypoxia in fibroblast cultures. 2. Influence of pH on the distribution pattern of acid mucopolysaccharides.
1975,
Pubmed
Koyama,
The enhanced production of hyaluronic acid by cultured rat fibroblast cells treated with cyclic AMP and its dibutyryl derivative.
1975,
Pubmed
LOWRY,
Protein measurement with the Folin phenol reagent.
1951,
Pubmed
Matalon,
Acid mucopolysaccharides in cultured fibroblasts of cystic fibrosis of the pancreas.
1968,
Pubmed
Mian,
Characterization of a high-Mr plasma-membrane-bound protein and assessment of its role as a constituent of hyaluronate synthase complex.
1986,
Pubmed
,
Xenbase
Pekarthy,
Function and control of liver alkaline phosphatase.
1972,
Pubmed
Prehm,
Induction of hyaluronic acid synthesis in teratocarcinoma stem cells by retinoic acid.
1980,
Pubmed
Prehm,
Synthesis of hyaluronate in differentiated teratocarcinoma cells. Mechanism of chain growth.
1983,
Pubmed
Prehm,
Hyaluronate is synthesized at plasma membranes.
1984,
Pubmed
Rubin,
Protein phosphorylation.
1975,
Pubmed
Sottocasa,
An electron-transport system associated with the outer membrane of liver mitochondria. A biochemical and morphological study.
1967,
Pubmed
Stoolmiller,
The biosynthesis of hyaluronic acid by Streptococcus.
1969,
Pubmed
Tajima,
The influence of calcium ions on the synthesis of collagen and glycosaminoglycans in human diploid cells in culture.
1981,
Pubmed
Tomida,
Induction of hyaluronic acid synthetase activity in rat fibroblasts by medium change of confluent cultures.
1975,
Pubmed
Tomida,
Effects of adenosine 3':5'-cyclic monophosphate and serum on synthesis of hyaluronic acid in confluent rat fibroblasts.
1977,
Pubmed
Toole,
Hyaluronate turnover during chondrogenesis in the developing chick limb and axial skeleton.
1972,
Pubmed
Toole,
Hyaluronate and invasiveness of the rabbit V2 carcinoma.
1979,
Pubmed
