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The RNA polymerase I transcription factor UBF has been identified in human, mouse, rat and Xenopus and the primary structure of the human protein has been determined. Human UBF was shown to contain four tandem homologies to the folding domains of the HMG1 and 2 proteins and hence to belong to a previously unrecognised family of 'HMG-box' transcription factors. Here, cDNA clones encoding the Xenopus laevis UBF (xUBF) have been isolated and sequenced. Northern and Southern blots revealed that in tissue culture cells, xUBF is coded on a single major mRNA size species by a small number of genes. The deduced primary structure of xUBF is highly homologous with the human protein except for a central deletion which removes most of one HMG-box. This explains the major size difference between the X. laevis and human proteins and may well explain their different transcriptional specificities. It is shown that xUBF contains 5 tandemly repeated HMG-boxes and that by analogy the human protein contains 6.
Bateman,
Footprinting of ribosomal RNA genes by transcription initiation factor and RNA polymerase I.
1985, Pubmed
Bateman,
Footprinting of ribosomal RNA genes by transcription initiation factor and RNA polymerase I.
1985,
Pubmed
Bateman,
Events during eucaryotic rRNA transcription initiation and elongation: conversion from the closed to the open promoter complex requires nucleotide substrates.
1988,
Pubmed
Bateman,
Regulation of eukaryotic ribosomal RNA transcription by RNA polymerase modification.
1986,
Pubmed
Bell,
Molecular mechanisms governing species-specific transcription of ribosomal RNA.
1989,
Pubmed
,
Xenbase
Bell,
Functional cooperativity between transcription factors UBF1 and SL1 mediates human ribosomal RNA synthesis.
1988,
Pubmed
Bell,
Assembly of alternative multiprotein complexes directs rRNA promoter selectivity.
1990,
Pubmed
Carballo,
DNA and histone H1 interact with different domains of HMG 1 and 2 proteins.
1983,
Pubmed
Cary,
Conformation and domain structure of the non-histone chromosomal proteins HMG 1 and 2. Domain interactions.
1984,
Pubmed
Cary,
Conformation and domain structure of the non-histone chromosomal proteins, HMG 1 and 2. Isolation of two folded fragments from HMG 1 and 2.
1983,
Pubmed
Churchill,
'SPKK' motifs prefer to bind to DNA at A/T-rich sites.
1989,
Pubmed
Corden,
Tails of RNA polymerase II.
1990,
Pubmed
Devereux,
A comprehensive set of sequence analysis programs for the VAX.
1984,
Pubmed
Feinberg,
"A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.
1984,
Pubmed
Feinberg,
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
1983,
Pubmed
Gribskov,
Sigma factors from E. coli, B. subtilis, phage SP01, and phage T4 are homologous proteins.
1986,
Pubmed
Gubbay,
A gene mapping to the sex-determining region of the mouse Y chromosome is a member of a novel family of embryonically expressed genes.
1990,
Pubmed
Jantzen,
Nucleolar transcription factor hUBF contains a DNA-binding motif with homology to HMG proteins.
1990,
Pubmed
Kozak,
An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs.
1987,
Pubmed
Learned,
Purification and characterization of a transcription factor that confers promoter specificity to human RNA polymerase I.
1985,
Pubmed
Learned,
Human rRNA transcription is modulated by the coordinate binding of two factors to an upstream control element.
1986,
Pubmed
Ma,
Deletion analysis of GAL4 defines two transcriptional activating segments.
1987,
Pubmed
Manfioletti,
A new and fast method for preparing high quality lambda DNA suitable for sequencing.
1988,
Pubmed
McMaster,
Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.
1977,
Pubmed
Pikaard,
The Xenopus ribosomal gene enhancers bind an essential polymerase I transcription factor, xUBF.
1989,
Pubmed
,
Xenbase
Pikaard,
rUBF, an RNA polymerase I transcription factor from rats, produces DNase I footprints identical to those produced by xUBF, its homolog from frogs.
1990,
Pubmed
,
Xenbase
Reeves,
The A.T-DNA-binding domain of mammalian high mobility group I chromosomal proteins. A novel peptide motif for recognizing DNA structure.
1990,
Pubmed
Riley,
Very rapid nucleotide sequence analysis of improved, double-stranded minipreps.
1989,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Schnapp,
Isolation and functional characterization of TIF-IB, a factor that confers promoter specificity to mouse RNA polymerase I.
1990,
Pubmed
Sinclair,
A gene from the human sex-determining region encodes a protein with homology to a conserved DNA-binding motif.
1990,
Pubmed
Singh,
High mobility group proteins 1 and 2 function as general class II transcription factors.
1990,
Pubmed
Smith,
Interaction of RNA polymerase I transcription factors with a promoter in the nontranscribed spacer of rat ribosomal DNA.
1990,
Pubmed
Smith,
Characterization of factors that direct transcription of rat ribosomal DNA.
1990,
Pubmed
Suzuki,
SPKK, a new nucleic acid-binding unit of protein found in histone.
1989,
Pubmed
Suzuki,
The heptad repeat in the largest subunit of RNA polymerase II binds by intercalating into DNA.
1990,
Pubmed
Suzuki,
SPXX, a frequent sequence motif in gene regulatory proteins.
1989,
Pubmed
Tanaka,
Sequence-specific binding of a transcription factor TFID to the promoter region of mouse ribosomal RNA gene.
1990,
Pubmed
Tower,
Factors and nucleotide sequences that direct ribosomal DNA transcription and their relationship to the stable transcription complex.
1986,
Pubmed
