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XB-ART-37247
Curr Protoc Mol Biol 2004 May 01;Chapter 14:Unit 14.9. doi: 10.1002/0471142727.mb1409s66.
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Whole-mount in situ hybridization and detection of RNAs in vertebrate embryos and isolated organs.

Pizard A , Haramis A , Carrasco AE , Franco P , López S , Paganelli A .


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Nonisotopic in situ hybridization using intact embryos or organs is an important method for determining the spatial distribution of RNAs. Because it allows the analysis of large numbers of samples, it is amenable to temporal expression studies and comparison between different genotypes. It offers sensitivity and reproducibility. In addition, histological details are not lost during the staining process. The protocols in this unit can be used for whole-mount in situ hybridization in Xenopus, mouse, and chicken embryos, as well as dissected organs from mouse and chicken. Preparation of digoxigenin-labeled riboprobes is also described.

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