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Synthesis and release of hyaluronic acid by Swiss 3T3 fibroblasts.
Kitchen JR
,
Cysyk RL
.
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Hyaluronic acid (HA) and its synthesis were studied in intact Swiss 3T3 mouse fibroblasts and isolated membranes. HA chains in culture medium, attached to cells and in isolated membranes, were determined to possess average M(r) values of 5.2 x 10(6), 1.8 x 10(6) and 0.14 x 10(6) respectively. Log cells were determined to possess 680,000 HA molecules/cell, and to release 120,000 HA chains/h. The time required for intact cells to synthesize and release a complete HA chain was approximately 4 h, with elongation proceeding at a rate of 57 dimers/min. The amount of cell-associated HA of various cell populations correlated strongly with their rate of HA release into culture media and with the HA synthetase activity determined for their membranes. Prevention of protein synthesis with cycloheximide decreased the rate of HA synthesis of log cells and HA synthetase activity of isolated membranes by 50% within 2-3 h. Because of the similarity between the biological lifetime of HA synthetase and the time required to synthesize a HA chain, we propose a model where each synthetase makes only one HA chain; after synthesis of a complete HA chain, HA synthetase activity is terminated as its HA chain is released from the cell.
Bansal,
Evidence for rapid metabolic turnover of hyaluronate synthetase in Swarm rat chondrosarcoma chondrocytes.
1986, Pubmed,
Xenbase
Bansal,
Evidence for rapid metabolic turnover of hyaluronate synthetase in Swarm rat chondrosarcoma chondrocytes.
1986,
Pubmed
,
Xenbase
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Goldenberg,
Increase in the affinity of the uridine phosphorylation system for ATP after serum or insulin activation of 3T3 fibroblasts.
1978,
Pubmed
Hadler,
Structure of hyaluronic acid in synovial fluid and its influence on the movement of solutes.
1977,
Pubmed
Heider,
A human homologue of the rat metastasis-associated variant of CD44 is expressed in colorectal carcinomas and adenomatous polyps.
1993,
Pubmed
Knudson,
Interactions between human tumor cells and fibroblasts stimulate hyaluronate synthesis.
1984,
Pubmed
Knudson,
Similar epithelial-stromal interactions in the regulation of hyaluronate production during limb morphogenesis and tumor invasion.
1990,
Pubmed
Laurent,
Determination of hyaluronate in biological samples by a specific radioassay technique.
1980,
Pubmed
Laurent,
Biochemistry of hyaluronan.
1987,
Pubmed
,
Xenbase
Lindahl,
Glycosaminoglycans and their binding to biological macromolecules.
1978,
Pubmed
Mausolf,
Shedding of hyaluronate synthase from streptococci.
1990,
Pubmed
,
Xenbase
Mian,
Analysis of cell-growth-phase-related variations in hyaluronate synthase activity of isolated plasma-membrane fractions of cultured human skin fibroblasts.
1986,
Pubmed
,
Xenbase
Ng,
Solubilization and partial purification of hyaluronate synthetase from oligodendroglioma cells.
1989,
Pubmed
,
Xenbase
Philipson,
Effect of hyaluronidase treatment of intact cells on hyaluronate synthetase activity.
1985,
Pubmed
,
Xenbase
Philipson,
Subcellular localization of hyaluronate synthetase in oligodendroglioma cells.
1984,
Pubmed
,
Xenbase
Prehm,
Hyaluronate is synthesized at plasma membranes.
1984,
Pubmed
Scott,
Secondary and tertiary structures of hyaluronan in aqueous solution, investigated by rotary shadowing-electron microscopy and computer simulation. Hyaluronan is a very efficient network-forming polymer.
1991,
Pubmed
Tengblad,
Quantitative analysis of hyaluronate in nanogram amounts.
1980,
Pubmed
Toole,
Developmental role of hyaluronate.
1982,
Pubmed
