Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
The hyaluronate synthase from a eukaryotic cell line.
Klewes L
,
Turley EA
,
Prehm P
.
???displayArticle.abstract???
The hyaluronate synthase complex was identified in plasma membranes from B6 cells. It contained two subunits of molecular masses 52 kDa and 60 kDa which bound the precursor UDP-GlcA in digitonin solution and partitioned into the aqueous phase, together with nascent hyaluronate upon Triton X-114 phase separation. The 52 kDa protein cross-reacted with poly- and monoclonal antibodies raised against the streptococcal hyaluronate synthase and the 60 kDa protein was recognized by monoclonal antibodies raised against a hyaluronate receptor. The 52 kDa protein was purified to homogeneity by affinity chromatography with monoclonal anti-hyaluronate synthase.
Brecht,
Increased hyaluronate synthesis is required for fibroblast detachment and mitosis.
1986, Pubmed,
Xenbase
Brecht,
Increased hyaluronate synthesis is required for fibroblast detachment and mitosis.
1986,
Pubmed
,
Xenbase
Engvall,
Enzyme immunoassay ELISA and EMIT.
1980,
Pubmed
Hardwick,
Molecular cloning of a novel hyaluronan receptor that mediates tumor cell motility.
1992,
Pubmed
Heusser,
Methods for binding cells to plastic: application to solid phase immunoassays for cell-surface antigens.
1981,
Pubmed
Koyama,
Isolation and characterization of hybrids between mouse and Chinese hamster cell lines.
1970,
Pubmed
Koyama,
Initiation of a differentiated function (hyaluronic acid synthesis) by hybrid formation in culture.
1970,
Pubmed
Mian,
Analysis of cell-growth-phase-related variations in hyaluronate synthase activity of isolated plasma-membrane fractions of cultured human skin fibroblasts.
1986,
Pubmed
,
Xenbase
Mian,
Characterization of a high-Mr plasma-membrane-bound protein and assessment of its role as a constituent of hyaluronate synthase complex.
1986,
Pubmed
,
Xenbase
Miyake,
Hyaluronate can function as a cell adhesion molecule and CD44 participates in hyaluronate recognition.
1990,
Pubmed
Ng,
Solubilization and partial purification of hyaluronate synthetase from oligodendroglioma cells.
1989,
Pubmed
,
Xenbase
Prehm,
Identification and regulation of the eukaryotic hyaluronate synthase.
1989,
Pubmed
,
Xenbase
Prehm,
Synthesis of hyaluronate in differentiated teratocarcinoma cells. Characterization of the synthase.
1983,
Pubmed
,
Xenbase
Prehm,
Isolation of streptococcal hyaluronate synthase.
1986,
Pubmed
,
Xenbase
Prehm,
Hyaluronate is synthesized at plasma membranes.
1984,
Pubmed
Tsang,
Enzyme-linked immunoelectrotransfer blot techniques (EITB) for studying the specificities of antigens and antibodies separated by gel electrophoresis.
1983,
Pubmed
Turley,
Hyaluronan and a cell-associated hyaluronan binding protein regulate the locomotion of ras-transformed cells.
1991,
Pubmed
Turley,
Characterization of hyaluronate binding proteins isolated from 3T3 and murine sarcoma virus transformed 3T3 cells.
1987,
Pubmed
Turley,
Localization of hyaluronate and hyaluronate-binding protein on motile and non-motile fibroblasts.
1985,
Pubmed
Wessel,
A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids.
1984,
Pubmed
