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The myogenic factors MyoD1 and myogenin contain a conserved region with similarity to the myc family of proto-oncogenes. To identify amphibian genes structurally and functionally related to these myogenic factors, we screened a Xenopus laevis embryo cDNA library under conditions of reduced stringency with probes corresponding to the myc-like helix-loop-helix motif of mouse MyoD1 and myogenin. Several distinct cDNAs that are highly related to each other and share extensive homology to MyoD1 were isolated. Transcripts from two of these genes, Xlmf1 and Xlmf25 (X. laevis myogenic factor), reach maximal levels of accumulation during gastrulation, remain at constant levels through early embryogenesis, and are found exclusively in skeletal muscles of adult frogs. The appearance of these transcripts early in development precedes the expression of cardiac alpha-actin, a molecular marker for mesoderm formation. A third cDNA, Xlmf11, contains an internal 351-base-pair deletion downstream of the myc homology region and encodes a truncated version of the protein encoded by Xlmf1. When expressed in mouse pluripotential stem cells, Xlmf1 activates the muscle cell differentiation program, resulting in expression of endogenous MyoD1, myogenin, and myosin heavy-chain genes and formation of multinucleated myotubes.
Ballantine,
Changes in protein synthesis during the development of Xenopus laevis.
1979, Pubmed,
Xenbase
Ballantine,
Changes in protein synthesis during the development of Xenopus laevis.
1979,
Pubmed
,
Xenbase
Boncinelli,
Organization of human homeobox genes.
1988,
Pubmed
Braun,
A novel human muscle factor related to but distinct from MyoD1 induces myogenic conversion in 10T1/2 fibroblasts.
1989,
Pubmed
Caudy,
daughterless, a Drosophila gene essential for both neurogenesis and sex determination, has sequence similarities to myc and the achaete-scute complex.
1988,
Pubmed
Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed
Cooke,
Evidence for specific feedback signals underlying pattern control during vertebrate embryogenesis.
1983,
Pubmed
,
Xenbase
Davis,
Expression of a single transfected cDNA converts fibroblasts to myoblasts.
1987,
Pubmed
Duboule,
A new homeo-box is present in overlapping cosmid clones which define the mouse Hox-1 locus.
1986,
Pubmed
,
Xenbase
Edmondson,
A gene with homology to the myc similarity region of MyoD1 is expressed during myogenesis and is sufficient to activate the muscle differentiation program.
1989,
Pubmed
Feinberg,
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
1983,
Pubmed
Gentz,
Parallel association of Fos and Jun leucine zippers juxtaposes DNA binding domains.
1989,
Pubmed
Hamilton,
The formation of somites in Xenopus.
1969,
Pubmed
,
Xenbase
Harvey,
Embryonic expression and nuclear localization of Xenopus homeobox (Xhox) gene products.
1986,
Pubmed
,
Xenbase
Hopwood,
MyoD expression in the forming somites is an early response to mesoderm induction in Xenopus embryos.
1989,
Pubmed
,
Xenbase
Jonas,
Epidermal keratin gene expressed in embryos of Xenopus laevis.
1985,
Pubmed
,
Xenbase
Kaufman,
Cytogenetic Analysis of Chromosome 3 in DROSOPHILA MELANOGASTER: The Homoeotic Gene Complex in Polytene Chromosome Interval 84a-B.
1980,
Pubmed
Kimes,
Characterization of two putative smooth muscle cell lines from rat thoracic aorta.
1976,
Pubmed
Kozak,
The scanning model for translation: an update.
1989,
Pubmed
Landschulz,
The DNA binding domain of the rat liver nuclear protein C/EBP is bipartite.
1989,
Pubmed
Lin,
An avian muscle factor related to MyoD1 activates muscle-specific promoters in nonmuscle cells of different germ-layer origin and in BrdU-treated myoblasts.
1989,
Pubmed
Melton,
Translation of messenger RNA in injected frog oocytes.
1987,
Pubmed
,
Xenbase
Mohun,
Cell type-specific activation of actin genes in the early amphibian embryo.
,
Pubmed
,
Xenbase
Murre,
A new DNA binding and dimerization motif in immunoglobulin enhancer binding, daughterless, MyoD, and myc proteins.
1989,
Pubmed
Pinney,
Myogenic lineage determination and differentiation: evidence for a regulatory gene pathway.
1988,
Pubmed
Rhodes,
Identification of MRF4: a new member of the muscle regulatory factor gene family.
1989,
Pubmed
Sánchez-Herrero,
Genetic organization of Drosophila bithorax complex.
,
Pubmed
Sassoon,
Expression of two myogenic regulatory factors myogenin and MyoD1 during mouse embryogenesis.
1989,
Pubmed
Slack,
An interaction between dorsal and ventral regions of the marginal zone in early amphibian embryos.
1980,
Pubmed
,
Xenbase
Smith,
Mesoderm induction and mesoderm-inducing factors in early amphibian development.
1989,
Pubmed
Stone,
Definition of regions in human c-myc that are involved in transformation and nuclear localization.
1987,
Pubmed
Tapscott,
MyoD1: a nuclear phosphoprotein requiring a Myc homology region to convert fibroblasts to myoblasts.
1988,
Pubmed
Thisse,
Sequence of the twist gene and nuclear localization of its protein in endomesodermal cells of early Drosophila embryos.
1988,
Pubmed
Turner,
Leucine repeats and an adjacent DNA binding domain mediate the formation of functional cFos-cJun heterodimers.
1989,
Pubmed
Villares,
The achaete-scute gene complex of D. melanogaster: conserved domains in a subset of genes required for neurogenesis and their homology to myc.
1987,
Pubmed
Winkles,
Developmentally regulated cytokeratin gene in Xenopus laevis.
1985,
Pubmed
,
Xenbase
Wright,
Myogenin, a factor regulating myogenesis, has a domain homologous to MyoD.
1989,
Pubmed
Yaffe,
Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle.
,
Pubmed
