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Postsynaptic differentiation can be experimentally induced in cultured Xenopus myotomal muscle cells by polyornithine-coated latex beads (Peng, H. B., and P.-C. Cheng, 1982, J. Neurosci., 2:1760-1774). In this study, we examined the time course of this process. Small, punctate acetylcholine receptor (AChR) clusters were detectable as early as 1.5 h after the addition of the beads. Subsequently, both the size and the number of the clusters increased with time until a saturation level was reached between 8-24 h. Because the onset and the site of the AChR clustering could be precisely marked, we were able to examine the early structural specializations associated with presumptive AChR clusters. At 1 h, when less than 20% bead-muscle contacts displayed AChR clusters, 70% of the contacts already exhibited a meshwork of 5-6-nm filaments, which were of the same size as the thin filaments within the myofibrils and thus may contain actin. A system of cisternae similar to the smooth endoplasmic reticulum was suspended within this meshwork, but other organelles were excluded from it. This meshwork, being the earliest cytoplasmic specialization at the presumptive AChR clusters and appearing before the clusters, may be a mechanism for the clustering process.
Anderson,
Nerve-induced and spontaneous redistribution of acetylcholine receptors on cultured muscle cells.
1977, Pubmed,
Xenbase
Anderson,
Nerve-induced and spontaneous redistribution of acetylcholine receptors on cultured muscle cells.
1977,
Pubmed
,
Xenbase
Bridgman,
Distribution of filipin-sterol complexes on cultured muscle cells: cell-substratum contact areas associated with acetylcholine receptor clusters.
1983,
Pubmed
,
Xenbase
Cohen,
Clusters of intramembrane particles associated with binding sites for alpha-bungarotoxin in cultured chick myotubes.
1979,
Pubmed
Edwards,
A model for the localization of acetylcholine receptors at the muscle endplate.
1976,
Pubmed
Endo,
Calcium release from the sarcoplasmic reticulum.
1977,
Pubmed
Evans,
The entry of labelled calcium into the innervated region of the mouse diaphragm muscle.
1974,
Pubmed
Frank,
Early events in neuromuscular junction formation in vitro: induction of acetylcholine receptor clusters in the postsynaptic membrane and morphology of newly formed synapses.
1979,
Pubmed
Gulley,
Cytoskeletal organization at the postsynaptic complex.
1981,
Pubmed
Hall,
Cytoplasmic actin in postsynaptic structures at the neuromuscular junction.
1981,
Pubmed
Heuser,
Organization of acetylcholine receptors in quick-frozen, deep-etched, and rotary-replicated Torpedo postsynaptic membrane.
1979,
Pubmed
Hirokawa,
Internal and external differentiations of the postsynaptic membrane at the neuromuscular junction.
1982,
Pubmed
Koppel,
Matrix control of protein diffusion in biological membranes.
1981,
Pubmed
Kreis,
Mobility of microinjected rhodamine actin within living chicken gizzard cells determined by fluorescence photobleaching recovery.
1982,
Pubmed
Luther,
Changes in cell shape and actin distribution induced by constant electric fields.
,
Pubmed
,
Xenbase
Olek,
Direct observation of the rapid aggregation of acetylcholine receptors on identified cultured myotubes after exposure to embryonic brain extract.
1983,
Pubmed
Ornberg,
A freeze-substitution method for localizing divalent cations: examples from secretory systems.
1980,
Pubmed
Painter,
Effects of cytochalasin B on actin and myosin association with particle binding sites in mouse macrophages: implications with regard to the mechanism of action of the cytochalasins.
1981,
Pubmed
Peng,
Participation of calcium and calmodulin in the formation of acetylcholine receptor clusters.
1984,
Pubmed
,
Xenbase
Peng,
Development of the postsynaptic membrane in Xenopus neuromuscular cultures observed by freeze-fracture and thin-section electron microscopy.
1980,
Pubmed
,
Xenbase
Peng,
Formation of ACh receptor clusters induced by positively charged latex beads.
1981,
Pubmed
,
Xenbase
Peng,
Membrane particle aggregates in innervated and noninnervated cultures of Xenopus embryonic muscle cells.
1978,
Pubmed
,
Xenbase
Peng,
Formation of postsynaptic specializations induced by latex beads in cultured muscle cells.
1982,
Pubmed
,
Xenbase
Peng,
Cytoskeletal organization of the presynaptic nerve terminal and the acetylcholine receptor cluster in cell cultures.
1983,
Pubmed
,
Xenbase
Poo,
Rapid lateral diffusion of functional A Ch receptors in embryonic muscle cell membrane.
1982,
Pubmed
,
Xenbase
Prives,
Interaction of the cytoskeletal framework with acetylcholine receptor on th surface of embryonic muscle cells in culture.
1982,
Pubmed
Ravdin,
Fluorescent tetramethyl rhodamine derivatives of alpha-bungarotoxin: preparation, separation, and characterization.
1977,
Pubmed
Salpeter,
Distribution and turnover rate of acetylcholine receptors throughout the junction folds at a vertebrate neuromuscular junction.
1983,
Pubmed
Small,
Organization of actin in the leading edge of cultured cells: influence of osmium tetroxide and dehydration on the ultrastructure of actin meshworks.
1981,
Pubmed
