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Science
2008 Jun 20;3205883:1655-8. doi: 10.1126/science.1157425.
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Bora and the kinase Aurora a cooperatively activate the kinase Plk1 and control mitotic entry.
Seki A
,
Coppinger JA
,
Jang CY
,
Yates JR
,
Fang G
.
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A central question in the study of cell proliferation is, what controls cell-cycle transitions? Although the accumulation of mitotic cyclins drives the transition from the G2 phase to the M phase in embryonic cells, the trigger for mitotic entry in somatic cells remains unknown. We report that the synergistic action of Bora and the kinase Aurora A (Aur-A) controls the G2-M transition. Bora accumulates in the G2 phase and promotes Aur-A-mediated activation of Polo-like kinase 1 (Plk1), leading to the activation of cyclin-dependent kinase 1 and mitotic entry. Mechanistically, Bora interacts with Plk1 and controls the accessibility of its activation loop for phosphorylation and activation by Aur-A. Thus, Bora and Aur-A control mitotic entry, which provides a mechanism for one of the most important yet ill-defined events in the cell cycle.
Barr,
Polo-like kinases and the orchestration of cell division.
2004, Pubmed
Barr,
Polo-like kinases and the orchestration of cell division.
2004,
Pubmed
Elia,
Proteomic screen finds pSer/pThr-binding domain localizing Plk1 to mitotic substrates.
2003,
Pubmed
Elia,
The molecular basis for phosphodependent substrate targeting and regulation of Plks by the Polo-box domain.
2003,
Pubmed
,
Xenbase
Erikson,
A feedback loop in the polo-like kinase activation pathway.
2004,
Pubmed
,
Xenbase
Ferrari,
Aurora-A site specificity: a study with synthetic peptide substrates.
2005,
Pubmed
Hansen,
Plk1 regulates activation of the anaphase promoting complex by phosphorylating and triggering SCFbetaTrCP-dependent destruction of the APC Inhibitor Emi1.
2004,
Pubmed
,
Xenbase
Harrington,
VX-680, a potent and selective small-molecule inhibitor of the Aurora kinases, suppresses tumor growth in vivo.
2004,
Pubmed
Hirota,
Aurora-A and an interacting activator, the LIM protein Ajuba, are required for mitotic commitment in human cells.
2003,
Pubmed
,
Xenbase
Hutterer,
Mitotic activation of the kinase Aurora-A requires its binding partner Bora.
2006,
Pubmed
Jang,
Phosphorylation of threonine 210 and the role of serine 137 in the regulation of mammalian polo-like kinase.
2002,
Pubmed
,
Xenbase
Jang,
Functional studies on the role of the C-terminal domain of mammalian polo-like kinase.
2002,
Pubmed
Kelm,
Cell cycle-regulated phosphorylation of the Xenopus polo-like kinase Plx1.
2002,
Pubmed
,
Xenbase
Kothe,
Structure of the catalytic domain of human polo-like kinase 1.
2007,
Pubmed
Kumagai,
Purification and molecular cloning of Plx1, a Cdc25-regulatory kinase from Xenopus egg extracts.
1996,
Pubmed
,
Xenbase
Kunitoku,
CENP-A phosphorylation by Aurora-A in prophase is required for enrichment of Aurora-B at inner centromeres and for kinetochore function.
2003,
Pubmed
Lee,
Plk is a functional homolog of Saccharomyces cerevisiae Cdc5, and elevated Plk activity induces multiple septation structures.
1997,
Pubmed
,
Xenbase
Lénárt,
The small-molecule inhibitor BI 2536 reveals novel insights into mitotic roles of polo-like kinase 1.
2007,
Pubmed
Liu,
The MAP kinase pathway is required for entry into mitosis and cell survival.
2004,
Pubmed
Liu,
Xenopus Polo-like kinase Plx1: a multifunctional mitotic kinase.
2005,
Pubmed
,
Xenbase
Murray,
Recycling the cell cycle: cyclins revisited.
2004,
Pubmed
Pugacheva,
The focal adhesion scaffolding protein HEF1 regulates activation of the Aurora-A and Nek2 kinases at the centrosome.
2005,
Pubmed
Qian,
The polo-like kinase Plx1 is required for activation of the phosphatase Cdc25C and cyclin B-Cdc2 in Xenopus oocytes.
2001,
Pubmed
,
Xenbase
Qian,
Mitotic effects of a constitutively active mutant of the Xenopus polo-like kinase Plx1.
1999,
Pubmed
,
Xenbase
Qian,
Purification and cloning of a protein kinase that phosphorylates and activates the polo-like kinase Plx1.
1998,
Pubmed
,
Xenbase
van Vugt,
Getting in and out of mitosis with Polo-like kinase-1.
2005,
Pubmed
Wang,
Polo inhibits progenitor self-renewal and regulates Numb asymmetry by phosphorylating Pon.
2007,
Pubmed
Watanabe,
M-phase kinases induce phospho-dependent ubiquitination of somatic Wee1 by SCFbeta-TrCP.
2004,
Pubmed
Whitfield,
Identification of genes periodically expressed in the human cell cycle and their expression in tumors.
2002,
Pubmed
Zhao,
The GIT-associated kinase PAK targets to the centrosome and regulates Aurora-A.
2005,
Pubmed
