Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Biol Chem
2011 Mar 25;28612:10803-13. doi: 10.1074/jbc.M110.179739.
Show Gene links
Show Anatomy links
AaCAT1 of the yellow fever mosquito, Aedes aegypti: a novel histidine-specific amino acid transporter from the SLC7 family.
Hansen IA
,
Boudko DY
,
Shiao SH
,
Voronov DA
,
Meleshkevitch EA
,
Drake LL
,
Aguirre SE
,
Fox JM
,
Attardo GM
,
Raikhel AS
.
???displayArticle.abstract???
Insect yolk protein precursor gene expression is regulated by nutritional and endocrine signals. A surge of amino acids in the hemolymph of blood-fed female mosquitoes activates a nutrient signaling system in the fat bodies, which subsequently derepresses yolk protein precursor genes and makes them responsive to activation by steroid hormones. Orphan transporters of the SLC7 family were identified as essential upstream components of the nutrient signaling system in the fat body of fruit flies and the yellow fever mosquito, Aedes aegypti. However, the transport function of these proteins was unknown. We report expression and functional characterization of AaCAT1, cloned from the fat body of A. aegypti. Expression of AaCAT1 transcript and protein undergoes dynamic changes during postembryonic development of the mosquito. Transcript expression was especially high in the third and fourth larval stages; however, the AaCAT1 protein was detected only in pupa and adult stages. Functional expression and analysis of AaCAT1 in Xenopus oocytes revealed that it acts as a sodium-independent cationic amino acid transporter, with unique selectivity to L-histidine at neutral pH (K(0.5)(L-His) = 0.34 ± 0.07 mM, pH 7.2). Acidification to pH 6.2 dramatically increases AaCAT1-specific His(+)-induced current. RNAi-mediated silencing of AaCAT1 reduces egg yield of subsequent ovipositions. Our data show that AaCAT1 has notable differences in its transport mechanism when compared with related mammalian cationic amino acid transporters. It may execute histidine-specific transport and signaling in mosquito tissues.
Altschul,
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
1997, Pubmed
Altschul,
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
1997,
Pubmed
Arrese,
Insect fat body: energy, metabolism, and regulation.
2010,
Pubmed
Attardo,
Nutritional regulation of vitellogenesis in mosquitoes: implications for anautogeny.
2005,
Pubmed
Attardo,
Identification of two cationic amino acid transporters required for nutritional signaling during mosquito reproduction.
2006,
Pubmed
Baker,
Diabetic larvae and obese flies-emerging studies of metabolism in Drosophila.
2007,
Pubmed
Bauch,
Apical heterodimeric cystine and cationic amino acid transporter expressed in MDCK cells.
2002,
Pubmed
,
Xenbase
Biessmann,
The Anopheles gambiae odorant binding protein 1 (AgamOBP1) mediates indole recognition in the antennae of female mosquitoes.
2010,
Pubmed
Boudko,
Ancestry and progeny of nutrient amino acid transporters.
2005,
Pubmed
,
Xenbase
Bryson,
Evolving Genes and Proteins.
1965,
Pubmed
Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed
Closs,
Structure and function of cationic amino acid transporters (CATs).
2006,
Pubmed
Colombani,
A nutrient sensor mechanism controls Drosophila growth.
2003,
Pubmed
Devés,
Transporters for cationic amino acids in animal cells: discovery, structure, and function.
1998,
Pubmed
,
Xenbase
Dissanayake,
aeGEPUCI: a database of gene expression in the dengue vector mosquito, Aedes aegypti.
2010,
Pubmed
Drake,
The Aquaporin gene family of the yellow fever mosquito, Aedes aegypti.
2010,
Pubmed
Edgar,
MUSCLE: multiple sequence alignment with high accuracy and high throughput.
2004,
Pubmed
Etoh,
Electrophysiological responses of Xenopus oocytes to amino acids: criteria for expression of injected mRNA coding chemoreceptors.
1994,
Pubmed
,
Xenbase
Felsenstein,
CONFIDENCE LIMITS ON PHYLOGENIES: AN APPROACH USING THE BOOTSTRAP.
1985,
Pubmed
Goberdhan,
PAT-related amino acid transporters regulate growth via a novel mechanism that does not require bulk transport of amino acids.
2005,
Pubmed
,
Xenbase
Gutierrez,
Specialized hepatocyte-like cells regulate Drosophila lipid metabolism.
2007,
Pubmed
Hansen,
Target of rapamycin-mediated amino acid signaling in mosquito anautogeny.
2004,
Pubmed
Hansen,
Target of rapamycin-dependent activation of S6 kinase is a central step in the transduction of nutritional signals during egg development in a mosquito.
2005,
Pubmed
Hediger,
The ABCs of solute carriers: physiological, pathological and therapeutic implications of human membrane transport proteinsIntroduction.
2004,
Pubmed
Hennig,
TOR coordinates bulk and targeted endocytosis in the Drosophila melanogaster fat body to regulate cell growth.
2006,
Pubmed
Kim,
Transport of cationic amino acids by the mouse ecotropic retrovirus receptor.
1991,
Pubmed
,
Xenbase
Martin,
minidiscs encodes a putative amino acid transporter subunit required non-autonomously for imaginal cell proliferation.
2000,
Pubmed
Meleshkevitch,
Molecular characterization of the first aromatic nutrient transporter from the sodium neurotransmitter symporter family.
2006,
Pubmed
,
Xenbase
Miller,
The invertebrate B(0) system transporter, D. melanogaster NAT1, has unique d-amino acid affinity and mediates gut and brain functions.
2008,
Pubmed
Miller,
The heterologous expression of H(+)-coupled transporters in Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Saitou,
The neighbor-joining method: a new method for reconstructing phylogenetic trees.
1987,
Pubmed
Shaffer,
Structure and mechanism of a Na+-independent amino acid transporter.
2009,
Pubmed
Tamura,
MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0.
2007,
Pubmed
Vékony,
Human cationic amino acid transporter hCAT-3 is preferentially expressed in peripheral tissues.
2001,
Pubmed
,
Xenbase
Verrey,
CATs and HATs: the SLC7 family of amino acid transporters.
2004,
Pubmed
