Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
The MAN antigens are non-lamin constituents of the nuclear lamina in vertebrate cells.
Paulin-Levasseur M
,
Blake DL
,
Julien M
,
Rouleau L
.
???displayArticle.abstract???
The characterization of the human antiserum designated MAN has led to the identification of a subset of non-lamin proteins that are exclusively located at the nuclear periphery in all vertebrate cell types examined, from human to fish. Immunoreactive protein species were shown to comprise three major polypeptides of Mr 78000, 58000 and 40000. These antigens co-partitioned with the nuclear lamina during in situ isolation of nuclear matrices from lamin A/C-positive and -negative mammalian cells. Using double immunofluorescence, the spatial relationship of MAN antigens to type-A and type-B lamins was further examined throughout the cell cycle of lamin A/C-positive mammalian cells. In interphase HeLa and 3T3 cells, MAN antigens colocalized with both types of lamins at the periphery of the nucleus, but were absent from intranuclear foci of lamin B. As HeLa cells proceeded into mitosis, MAN antigens were seen to segregate from lamins A/C and coredistribute with lamin B. Lamins A/C disassembled during late prophase/early prometaphase and reassociated with chromatin in telophase/cytokinesis. In contrast, MAN antigens and lamin B dispersed late during prometaphase and reassembled on chromosomes in anaphase. Altogether, our data suggest that MAN antigens may play key functions in the maintenance of the structural integrity of the nuclear compartment in vertebrate cells.
Aaronson,
Isolation of nuclear pore complexes in association with a lamina.
1975, Pubmed
Aaronson,
Isolation of nuclear pore complexes in association with a lamina.
1975,
Pubmed
Appelbaum,
In vivo phosphorylation of the lamin B receptor. Binding of lamin B to its nuclear membrane receptor is affected by phosphorylation.
1990,
Pubmed
Bailer,
Characterization of A 54-kD protein of the inner nuclear membrane: evidence for cell cycle-dependent interaction with the nuclear lamina.
1991,
Pubmed
Benavente,
Involvement of nuclear lamins in postmitotic reorganization of chromatin as demonstrated by microinjection of lamin antibodies.
1986,
Pubmed
Birnboim,
Fluorometric analysis of DNA unwinding to study strand breaks and repair in mammalian cells.
1990,
Pubmed
Bossie,
A cDNA from Drosophila melanogaster encodes a lamin C-like intermediate filament protein.
1993,
Pubmed
Burke,
A cell free system to study reassembly of the nuclear envelope at the end of mitosis.
1986,
Pubmed
Burke,
A monoclonal antibody which recognises each of the nuclear lamin polypeptides in mammalian cells.
1983,
Pubmed
Chaly,
Localization of nuclear antigens during preparation of nuclear matrices in situ.
1985,
Pubmed
Chaly,
Changes in distribution of nuclear matrix antigens during the mitotic cell cycle.
1984,
Pubmed
Chaudhary,
Stepwise reassembly of the nuclear envelope at the end of mitosis.
1993,
Pubmed
Courvalin,
Characterization of human autoantibodies specific for lamin A.
1990,
Pubmed
Fields,
A major 62-kD intranuclear matrix polypeptide is a component of metaphase chromosomes.
1988,
Pubmed
Fisher,
cDNA sequencing of nuclear lamins A and C reveals primary and secondary structural homology to intermediate filament proteins.
1986,
Pubmed
Foisner,
Integral membrane proteins of the nuclear envelope interact with lamins and chromosomes, and binding is modulated by mitotic phosphorylation.
1993,
Pubmed
Franke,
Nuclear lamins and cytoplasmic intermediate filament proteins: a growing multigene family.
1987,
Pubmed
Furukawa,
cDNA cloning of a germ cell specific lamin B3 from mouse spermatocytes and analysis of its function by ectopic expression in somatic cells.
1993,
Pubmed
Furukawa,
Cloning of a cDNA for lamina-associated polypeptide 2 (LAP2) and identification of regions that specify targeting to the nuclear envelope.
1995,
Pubmed
Georgatos,
Lamins and lamin-associated proteins.
1994,
Pubmed
Gerace,
The nuclear envelope lamina is reversibly depolymerized during mitosis.
1980,
Pubmed
Gerace,
Functional organization of the nuclear envelope.
1988,
Pubmed
Gerace,
Immunocytochemical localization of the major polypeptides of the nuclear pore complex-lamina fraction. Interphase and mitotic distribution.
1978,
Pubmed
Harel,
Persistence of major nuclear envelope antigens in an envelope-like structure during mitosis in Drosophila melanogaster embryos.
1989,
Pubmed
Höger,
Amino acid sequence and molecular characterization of murine lamin B as deduced from cDNA clones.
1988,
Pubmed
,
Xenbase
Höger,
Characterization of a second highly conserved B-type lamin present in cells previously thought to contain only a single B-type lamin.
1990,
Pubmed
,
Xenbase
Höger,
Interaction of Xenopus lamins A and LII with chromatin in vitro mediated by a sequence element in the carboxyterminal domain.
1991,
Pubmed
,
Xenbase
Kaufmann,
Additional members of the rat liver lamin polypeptide family. Structural and immunological characterization.
1989,
Pubmed
Kouklis,
Transient arrest of 3T3 cells in mitosis and inhibition of nuclear lamin reassembly around chromatin induced by anti-vimentin antibodies.
1993,
Pubmed
Krohne,
The nuclear lamins. A multigene family of proteins in evolution and differentiation.
1986,
Pubmed
,
Xenbase
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lebel,
Lamins A and C appear during retinoic acid-induced differentiation of mouse embryonal carcinoma cells.
1987,
Pubmed
Lehner,
Differential expression of nuclear lamin proteins during chicken development.
1987,
Pubmed
,
Xenbase
Lehner,
The nuclear lamin protein family in higher vertebrates. Identification of quantitatively minor lamin proteins by monoclonal antibodies.
1986,
Pubmed
Lischwe,
A new method for partial peptide mapping using N-chlorosuccinimide/urea and peptide silver staining in sodium dodecyl sulfate-polyacrylamide gels.
1982,
Pubmed
Martin,
cDNA cloning and characterization of lamina-associated polypeptide 1C (LAP1C), an integral protein of the inner nuclear membrane.
1995,
Pubmed
McKeon,
Homologies in both primary and secondary structure between nuclear envelope and intermediate filament proteins.
,
Pubmed
Meier,
Type B lamins remain associated with the integral nuclear envelope protein p58 during mitosis: implications for nuclear reassembly.
1994,
Pubmed
Moir,
Dynamic properties of nuclear lamins: lamin B is associated with sites of DNA replication.
1994,
Pubmed
Moir,
Lamin dynamics.
1993,
Pubmed
Newport,
Disassembly of the nucleus in mitotic extracts: membrane vesicularization, lamin disassembly, and chromosome condensation are independent processes.
1987,
Pubmed
,
Xenbase
Nigg,
Assembly-disassembly of the nuclear lamina.
1992,
Pubmed
Nigg,
The nuclear envelope.
1989,
Pubmed
O'Farrell,
High resolution two-dimensional electrophoresis of basic as well as acidic proteins.
1977,
Pubmed
Padan,
Isolation and characterization of the Drosophila nuclear envelope otefin cDNA.
1990,
Pubmed
Paddy,
Interphase nuclear envelope lamins form a discontinuous network that interacts with only a fraction of the chromatin in the nuclear periphery.
1990,
Pubmed
Paulin-Levasseur,
Lack of lamins A and C in mammalian hemopoietic cell lines devoid of intermediate filament proteins.
1988,
Pubmed
Paulin-Levasseur,
Expression of vimentin and nuclear lamins during the in vitro differentiation of human promyelocytic leukemia cells HL-60.
1989,
Pubmed
Paulin-Levasseur,
Expression of nuclear lamins in mammalian somatic cells lacking cytoplasmic intermediate filament proteins.
1989,
Pubmed
Powell,
Internuclear exchange of an inner nuclear membrane protein (p55) in heterokaryons: in vivo evidence for the interaction of p55 with the nuclear lamina.
1990,
Pubmed
Reid,
The effects and physiological consequences of raised levels of cortisol on rainbow trout (Oncorhynchus mykiss) erythrocyte beta-adrenoreceptors.
1991,
Pubmed
Röber,
Cells of the cellular immune and hemopoietic system of the mouse lack lamins A/C: distinction versus other somatic cells.
1990,
Pubmed
Röber,
Differential timing of nuclear lamin A/C expression in the various organs of the mouse embryo and the young animal: a developmental study.
1989,
Pubmed
Senior,
Integral membrane proteins specific to the inner nuclear membrane and associated with the nuclear lamina.
1988,
Pubmed
Shoeman,
Gene expression in Acetabularia. I. Calibration of wheat germ cell-free translation system proteins as internal references for two-dimensional electrophoresis.
1982,
Pubmed
Smith,
Identification of a short nuclear lamin protein selectively expressed during meiotic stages of rat spermatogenesis.
1992,
Pubmed
Stewart,
Teratocarcinoma stem cells and early mouse embryos contain only a single major lamin polypeptide closely resembling lamin B.
1987,
Pubmed
Stick,
The fates of chicken nuclear lamin proteins during mitosis: evidence for a reversible redistribution of lamin B2 between inner nuclear membrane and elements of the endoplasmic reticulum.
1988,
Pubmed
Sudhakar,
Stage-dependent changes in localization of a germ cell-specific lamin during mammalian spermatogenesis.
1990,
Pubmed
Traub,
Differential sensitivity of vimentin and nuclear lamins from Ehrlich ascites tumor cells toward Ca2+ -activated neutral thiol proteinase.
1988,
Pubmed
Ulitzur,
Lamin activity is essential for nuclear envelope assembly in a Drosophila embryo cell-free extract.
1992,
Pubmed
Vester,
Presence of a nuclear lamina in pachytene spermatocytes of the rat.
1993,
Pubmed
Wang,
Resinless section immunogold electron microscopy of karyo-cytoskeletal frameworks of eukaryotic cells cultured in vitro. Absence of a salt-stable nuclear matrix from mouse plasmacytoma MPC-11 cells.
1991,
Pubmed
Wolin,
A new lamin in Xenopus somatic tissues displays strong homology to human lamin A.
1987,
Pubmed
,
Xenbase
Worman,
Nuclear lamina heterogeneity in mammalian cells. Differential expression of the major lamins and variations in lamin B phosphorylation.
1988,
Pubmed
Worman,
The lamin B receptor of the nuclear envelope inner membrane: a polytopic protein with eight potential transmembrane domains.
1990,
Pubmed
Worman,
A lamin B receptor in the nuclear envelope.
1988,
Pubmed
Zewe,
Gene structure and chromosomal localization of the murine lamin B2 gene.
1991,
Pubmed
,
Xenbase
