Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Biol
1995 Jun 01;156:2972-82. doi: 10.1128/MCB.15.6.2972.
Show Gene links
Show Anatomy links
EFIA/YB-1 is a component of cardiac HF-1A binding activity and positively regulates transcription of the myosin light-chain 2v gene.
Zou Y
,
Chien KR
.
???displayArticle.abstract???
Transient assays in cultured ventricular muscle cells and studies in transgenic mice have identified two adjacent regulatory elements (HF-1a and HF-1b/MEF-2) as required to maintain ventricular chamber-specific expression of the myosin light-chain 2v (MLC-2v) gene. A rat neonatal heart cDNA library was screened with an HF-1a binding site, resulting in the isolation of EFIA, the rat homolog of human YB-1. Purified recombinant EFIA/YB-1 protein binds to the HF-1a site in a sequence-specific manner and contacts a subset of the HF-1a contact points made by the cardiac nuclear factor(s). The HF-1a sequence contains AGTGG, which is highly homologous to the inverted CCAAT core of the EFIA/YB-1 binding sites and is found to be essential for binding of the recombinant EFIA/YB-1. Antiserum against Xenopus YB-3 (100% identical in the DNA binding domain and 89% identical in overall amino acid sequence to rat EFIA) can specifically abolish a component of the endogenous HF-1a complex in the rat cardiac myocyte nuclear extracts. In cotransfection assays, EFIA/YB-1 increased 250-bp MLC-2v promoter activity by 3.4-fold specifically in the cardiac cell context and in an HF-1a site-dependent manner. EFIA/YB-1 complexes with an unknown protein in cardiac myocyte nuclear extracts to form the endogenous HF-1a binding activity. Immunocoprecipitation revealed that EFIA/YB-1 has a major associated protein of approximately 30 kDa (p30) in cardiac muscle cells. This study suggests that EFIA/YB-1, together with the partner p30, binds to the HF-1a site and, in conjunction with HF-1b/MEF-2, mediates ventricular chamber-specific expression of the MLC-2v gene.
Andersson,
Cloning, structure, and expression of the mitochondrial cytochrome P-450 sterol 26-hydroxylase, a bile acid biosynthetic enzyme.
1989, Pubmed
Andersson,
Cloning, structure, and expression of the mitochondrial cytochrome P-450 sterol 26-hydroxylase, a bile acid biosynthetic enzyme.
1989,
Pubmed
Bouvet,
A role for transcription and FRGY2 in masking maternal mRNA within Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Braun,
A novel human muscle factor related to but distinct from MyoD1 induces myogenic conversion in 10T1/2 fibroblasts.
1989,
Pubmed
Braun,
Myf-6, a new member of the human gene family of myogenic determination factors: evidence for a gene cluster on chromosome 12.
1990,
Pubmed
Chang,
Identification of a large bent DNA domain and binding sites for serum response factor adjacent to the NFI repeat cluster and enhancer region in the major IE94 promoter from simian cytomegalovirus.
1993,
Pubmed
Chen,
High-efficiency transformation of mammalian cells by plasmid DNA.
1987,
Pubmed
Chien,
Transcriptional regulation during cardiac growth and development.
1993,
Pubmed
Chien,
Molecular advances in cardiovascular biology.
1993,
Pubmed
Chodosh,
Human CCAAT-binding proteins have heterologous subunits.
1988,
Pubmed
Cohen,
The Xenopus YB3 protein binds the B box element of the class III promoter.
1991,
Pubmed
,
Xenbase
Danilition,
Transcription factor binding and spacing constraints in the human beta-actin proximal promoter.
1991,
Pubmed
Davis,
Expression of a single transfected cDNA converts fibroblasts to myoblasts.
1987,
Pubmed
Didier,
Characterization of the cDNA encoding a protein binding to the major histocompatibility complex class II Y box.
1988,
Pubmed
Edmondson,
A gene with homology to the myc similarity region of MyoD1 is expressed during myogenesis and is sufficient to activate the muscle differentiation program.
1989,
Pubmed
Evans,
Heterokaryons of cardiac myocytes and fibroblasts reveal the lack of dominance of the cardiac muscle phenotype.
1994,
Pubmed
Faber,
Rous sarcoma virus enhancer factor I is a ubiquitous CCAAT transcription factor highly related to CBF and NF-Y.
1990,
Pubmed
Farrance,
M-CAT binding factor is related to the SV40 enhancer binding factor, TEF-1.
1992,
Pubmed
Framson,
A serum response element and a binding site for NF-Y mediate the serum response of the human thrombospondin 1 gene.
1993,
Pubmed
Goldstein,
Major cold shock protein of Escherichia coli.
1990,
Pubmed
Grant,
Cloning and characterization of chicken YB-1: regulation of expression in the liver.
1993,
Pubmed
Gupta,
An E-box/M-CAT hybrid motif and cognate binding protein(s) regulate the basal muscle-specific and cAMP-inducible expression of the rat cardiac alpha-myosin heavy chain gene.
1994,
Pubmed
Hasegawa,
DNA binding properties of YB-1 and dbpA: binding to double-stranded, single-stranded, and abasic site containing DNAs.
1991,
Pubmed
Hidaka,
The MEF-3 motif is required for MEF-2-mediated skeletal muscle-specific induction of the rat aldolase A gene.
1993,
Pubmed
Iannello,
Characterization of a promoter element required for transcription in myocardial cells.
1991,
Pubmed
Iwaki,
Alpha- and beta-adrenergic stimulation induces distinct patterns of immediate early gene expression in neonatal rat myocardial cells. fos/jun expression is associated with sarcomere assembly; Egr-1 induction is primarily an alpha 1-mediated response.
1990,
Pubmed
Lee,
Positive regulatory elements (HF-1a and HF-1b) and a novel negative regulatory element (HF-3) mediate ventricular muscle-specific expression of myosin light-chain 2-luciferase fusion genes in transgenic mice.
1994,
Pubmed
Lee,
Myosin light chain-2 luciferase transgenic mice reveal distinct regulatory programs for cardiac and skeletal muscle-specific expression of a single contractile protein gene.
1992,
Pubmed
Lin,
An avian muscle factor related to MyoD1 activates muscle-specific promoters in nonmuscle cells of different germ-layer origin and in BrdU-treated myoblasts.
1989,
Pubmed
Lipson,
S-phase-specific regulation by deletion mutants of the human thymidine kinase promoter.
1989,
Pubmed
Lyons,
Developmental regulation of myosin gene expression in mouse cardiac muscle.
1990,
Pubmed
Mar,
M-CAT binding factor, a novel trans-acting factor governing muscle-specific transcription.
1990,
Pubmed
Mar,
A conserved CATTCCT motif is required for skeletal muscle-specific activity of the cardiac troponin T gene promoter.
1988,
Pubmed
Miner,
Herculin, a fourth member of the MyoD family of myogenic regulatory genes.
1990,
Pubmed
Molkentin,
Myocyte-specific enhancer-binding factor (MEF-2) regulates alpha-cardiac myosin heavy chain gene expression in vitro and in vivo.
1993,
Pubmed
Molkentin,
Expression of the alpha-myosin heavy chain gene in the heart is regulated in part by an E-box-dependent mechanism.
1993,
Pubmed
Moss,
The avian cardiac alpha-actin promoter is regulated through a pair of complex elements composed of E boxes and serum response elements that bind both positive- and negative-acting factors.
1994,
Pubmed
Navankasattusas,
A ubiquitous factor (HF-1a) and a distinct muscle factor (HF-1b/MEF-2) form an E-box-independent pathway for cardiac muscle gene expression.
1992,
Pubmed
Navankasattusas,
The basic helix-loop-helix protein upstream stimulating factor regulates the cardiac ventricular myosin light-chain 2 gene via independent cis regulatory elements.
1994,
Pubmed
O'Brien,
Positional specification of ventricular myosin light chain 2 expression in the primitive murine heart tube.
1993,
Pubmed
Olson,
MyoD family: a paradigm for development?
1990,
Pubmed
Ozer,
Isolation and characterization of a cDNA clone for the CCAAT transcription factor EFIA reveals a novel structural motif.
1990,
Pubmed
,
Xenbase
Panek,
Tumor necrosis factor alpha response elements in the HLA-DRA promoter: identification of a tumor necrosis factor alpha-induced DNA-protein complex in astrocytes.
1992,
Pubmed
Patwardhan,
EGR3, a novel member of the Egr family of genes encoding immediate-early transcription factors.
1991,
Pubmed
Pearson,
Human DNA polymerase alpha gene: sequences controlling expression in cycling and serum-stimulated cells.
1991,
Pubmed
Qasba,
A single transcription factor binds to two divergent sequence elements with a common function in cardiac myosin light chain-2 promoter.
1992,
Pubmed
Ranjan,
Masking mRNA from translation in somatic cells.
1993,
Pubmed
,
Xenbase
Rhodes,
Identification of MRF4: a new member of the muscle regulatory factor gene family.
1989,
Pubmed
Rudnicki,
MyoD or Myf-5 is required for the formation of skeletal muscle.
1993,
Pubmed
Ruiz-Lozano,
The gene encoding rat phosphoglycerate mutase subunit M: cloning and promoter analysis in skeletal muscle cells.
1994,
Pubmed
Sabath,
cDNA cloning and characterization of interleukin 2-induced genes in a cloned T helper lymphocyte.
1990,
Pubmed
Sakura,
Two human genes isolated by a novel method encode DNA-binding proteins containing a common region of homology.
1988,
Pubmed
Sartorelli,
Myocardial activation of the human cardiac alpha-actin promoter by helix-loop-helix proteins.
1992,
Pubmed
Scales,
Two distinct Xenopus genes with homology to MyoD1 are expressed before somite formation in early embryogenesis.
1990,
Pubmed
,
Xenbase
Sen,
Terminally differentiated neonatal rat myocardial cells proliferate and maintain specific differentiated functions following expression of SV40 large T antigen.
1988,
Pubmed
Studier,
Use of T7 RNA polymerase to direct expression of cloned genes.
1990,
Pubmed
Sturm,
A 100-kD HeLa cell octamer binding protein (OBP100) interacts differently with two separate octamer-related sequences within the SV40 enhancer.
1987,
Pubmed
Tafuri,
Xenopus Y-box transcription factors: molecular cloning, functional analysis and developmental regulation.
1990,
Pubmed
,
Xenbase
Tafuri,
DNA binding, multimerization, and transcription stimulation by the Xenopus Y box proteins in vitro.
1992,
Pubmed
,
Xenbase
Travali,
Structure of the human gene for the proliferating cell nuclear antigen.
1989,
Pubmed
Vinson,
In situ detection of sequence-specific DNA binding activity specified by a recombinant bacteriophage.
1988,
Pubmed
Weintraub,
The MyoD family and myogenesis: redundancy, networks, and thresholds.
1993,
Pubmed
Wistow,
Cold shock and DNA binding.
1990,
Pubmed
Wolffe,
The Y-box factors: a family of nucleic acid binding proteins conserved from Escherichia coli to man.
1992,
Pubmed
Wolffe,
Structural and functional properties of the evolutionarily ancient Y-box family of nucleic acid binding proteins.
1994,
Pubmed
Wright,
Myogenin, a factor regulating myogenesis, has a domain homologous to MyoD.
1989,
Pubmed
Yan,
Molecular cloning and characterization of interferon alpha/beta response element binding factors of the murine (2'-5')oligoadenylate synthetase ME-12 gene.
1991,
Pubmed
Yu,
Human myocyte-specific enhancer factor 2 comprises a group of tissue-restricted MADS box transcription factors.
1992,
Pubmed
Zeleznik-Le,
Affinity-purified CCAAT-box-binding protein (YEBP) functionally regulates expression of a human class II major histocompatibility complex gene and the herpes simplex virus thymidine kinase gene.
1991,
Pubmed
Zhu,
A novel, tissue-restricted zinc finger protein (HF-1b) binds to the cardiac regulatory element (HF-1b/MEF-2) in the rat myosin light-chain 2 gene.
1993,
Pubmed
Zhu,
A conserved 28-base-pair element (HF-1) in the rat cardiac myosin light-chain-2 gene confers cardiac-specific and alpha-adrenergic-inducible expression in cultured neonatal rat myocardial cells.
1991,
Pubmed
