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GATA-6 is expressed in presumptive cardiac mesoderm before gastrulation, but its role in heart development has been unclear. Here we show that Xenopus and zebrafish embryos, injected with antisense morpholino oligonucleotides designed specifically to knock-down translation of GATA-6 protein, are severely compromised for heart development. Injected embryos express greatly reduced levels of contractile machinery genes and, at the same stage, of regulatory genes such as bone morphogenetic protein-4 (BMP-4) and the Nkx2 family. In contrast, initial BMP and Nkx2 expression is normal, suggesting a maintenance role for GATA-6. Endoderm is critical for heart formation in several vertebrates including Xenopus, and separate perturbation of GATA-6 expression in the deepanterior endoderm and in the overlying heartmesoderm shows that GATA-6 is required in both for cardiogenesis. The GATA-6 requirement in cardiac mesoderm was confirmed in zebrafish, an organism in which endoderm is thought not to be necessary for heart formation. We therefore conclude that proper maturation of cardiac mesoderm requires GATA-6, which functions to maintain BMP-4 and Nkx2 expression.
Alexander,
A molecular pathway leading to endoderm formation in zebrafish.
1999, Pubmed
Alexander,
A molecular pathway leading to endoderm formation in zebrafish.
1999,
Pubmed
Andrée,
BMP-2 induces ectopic expression of cardiac lineage markers and interferes with somite formation in chicken embryos.
1998,
Pubmed
Attisano,
Smads as transcriptional co-modulators.
2000,
Pubmed
Blokzijl,
Physical and functional interaction between GATA-3 and Smad3 allows TGF-beta regulation of GATA target genes.
2002,
Pubmed
Brewer,
The human and mouse GATA-6 genes utilize two promoters and two initiation codons.
1999,
Pubmed
Brewer,
Widespread expression of an extended peptide sequence of GATA-6 during murine embryogenesis and non-equivalence of RNA and protein expression domains.
2002,
Pubmed
Charron,
Cooperative interaction between GATA-4 and GATA-6 regulates myocardial gene expression.
1999,
Pubmed
Charron,
GATA transcription factors and cardiac development.
1999,
Pubmed
,
Xenbase
Clement,
Bone morphogenetic protein 2 in the early development of Xenopus laevis.
1995,
Pubmed
,
Xenbase
Cripps,
Control of cardiac development by an evolutionarily conserved transcriptional network.
2002,
Pubmed
Dale,
Fate map for the 32-cell stage of Xenopus laevis.
1987,
Pubmed
,
Xenbase
Davis,
An Nkx-dependent enhancer regulates cGATA-6 gene expression during early stages of heart development.
2000,
Pubmed
,
Xenbase
Fu,
Vertebrate tinman homologues XNkx2-3 and XNkx2-5 are required for heart formation in a functionally redundant manner.
1998,
Pubmed
,
Xenbase
Gerber,
A role for the RNA-binding protein, hermes, in the regulation of heart development.
2002,
Pubmed
,
Xenbase
Gove,
Over-expression of GATA-6 in Xenopus embryos blocks differentiation of heart precursors.
1997,
Pubmed
,
Xenbase
Grépin,
Enhanced cardiogenesis in embryonic stem cells overexpressing the GATA-4 transcription factor.
1997,
Pubmed
Grépin,
Inhibition of transcription factor GATA-4 expression blocks in vitro cardiac muscle differentiation.
1995,
Pubmed
Grow,
Tinman function is essential for vertebrate heart development: elimination of cardiac differentiation by dominant inhibitory mutants of the tinman-related genes, XNkx2-3 and XNkx2-5.
1998,
Pubmed
,
Xenbase
Jiang,
Common role for each of the cGATA-4/5/6 genes in the regulation of cardiac morphogenesis.
1998,
Pubmed
Jiang,
The Xenopus GATA-4/5/6 genes are associated with cardiac specification and can regulate cardiac-specific transcription during embryogenesis.
1996,
Pubmed
,
Xenbase
Jones,
DVR-4 (bone morphogenetic protein-4) as a posterior-ventralizing factor in Xenopus mesoderm induction.
1992,
Pubmed
,
Xenbase
Koutsourakis,
The transcription factor GATA6 is essential for early extraembryonic development.
1999,
Pubmed
Kuo,
GATA4 transcription factor is required for ventral morphogenesis and heart tube formation.
1997,
Pubmed
Liberatore,
Nkx-2.5 gene induction in mice is mediated by a Smad consensus regulatory region.
2002,
Pubmed
,
Xenbase
Lien,
Control of early cardiac-specific transcription of Nkx2-5 by a GATA-dependent enhancer.
1999,
Pubmed
,
Xenbase
Lien,
Cardiac-specific activity of an Nkx2-5 enhancer requires an evolutionarily conserved Smad binding site.
2002,
Pubmed
,
Xenbase
Mohun,
Cell type-specific activation of actin genes in the early amphibian embryo.
,
Pubmed
,
Xenbase
Mohun,
Early steps in vertebrate cardiogenesis.
1997,
Pubmed
Molkentin,
Direct activation of a GATA6 cardiac enhancer by Nkx2.5: evidence for a reinforcing regulatory network of Nkx2.5 and GATA transcription factors in the developing heart.
2000,
Pubmed
,
Xenbase
Molkentin,
Requirement of the transcription factor GATA4 for heart tube formation and ventral morphogenesis.
1997,
Pubmed
Molkentin,
The zinc finger-containing transcription factors GATA-4, -5, and -6. Ubiquitously expressed regulators of tissue-specific gene expression.
2000,
Pubmed
Moody,
Fates of the blastomeres of the 32-cell-stage Xenopus embryo.
1987,
Pubmed
,
Xenbase
Morrisey,
GATA6 regulates HNF4 and is required for differentiation of visceral endoderm in the mouse embryo.
1998,
Pubmed
Narita,
The gene for transcription factor GATA-6 resides on mouse chromosome 18 and is expressed in myocardium and vascular smooth muscle.
1996,
Pubmed
Nascone,
An inductive role for the endoderm in Xenopus cardiogenesis.
1995,
Pubmed
,
Xenbase
Neave,
A graded response to BMP-4 spatially coordinates patterning of the mesoderm and ectoderm in the zebrafish.
1997,
Pubmed
Nemer,
Transcriptional activation of BMP-4 and regulation of mammalian organogenesis by GATA-4 and -6.
2003,
Pubmed
Newman,
tinman-related genes expressed during heart development in Xenopus.
1998,
Pubmed
,
Xenbase
Patient,
The GATA family (vertebrates and invertebrates).
2002,
Pubmed
Read,
Evidence for non-axial A/P patterning in the nonneural ectoderm of Xenopus and zebrafish pregastrula embryos.
1998,
Pubmed
,
Xenbase
Reiter,
Gata5 is required for the development of the heart and endoderm in zebrafish.
1999,
Pubmed
,
Xenbase
Sater,
The restriction of the heart morphogenetic field in Xenopus laevis.
1990,
Pubmed
,
Xenbase
Schultheiss,
A role for bone morphogenetic proteins in the induction of cardiac myogenesis.
1997,
Pubmed
Shi,
BMP signaling is required for heart formation in vertebrates.
2000,
Pubmed
,
Xenbase
Suzuki,
Differential expression of Xenopus BMPs in early embryos and tissues.
1993,
Pubmed
,
Xenbase
Walters,
Bone morphogenetic protein function is required for terminal differentiation of the heart but not for early expression of cardiac marker genes.
2001,
Pubmed
,
Xenbase
Weber,
A role for GATA5 in Xenopus endoderm specification.
2000,
Pubmed
,
Xenbase
Zon,
Expression of GATA-binding proteins during embryonic development in Xenopus laevis.
1991,
Pubmed
,
Xenbase
