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Plant Cell
2012 Jan 01;241:245-58. doi: 10.1105/tpc.111.092221.
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The Arabidopsis nitrate transporter NRT2.4 plays a double role in roots and shoots of nitrogen-starved plants.
Kiba T
,
Feria-Bourrellier AB
,
Lafouge F
,
Lezhneva L
,
Boutet-Mercey S
,
Orsel M
,
Bréhaut V
,
Miller A
,
Daniel-Vedele F
,
Sakakibara H
,
Krapp A
.
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Plants have evolved a variety of mechanisms to adapt to N starvation. NITRATE TRANSPORTER2.4 (NRT2.4) is one of seven NRT2 family genes in Arabidopsis thaliana, and NRT2.4 expression is induced under N starvation. Green fluorescent protein and β-glucuronidase reporter analyses revealed that NRT2.4 is a plasma membrane transporter expressed in the epidermis of lateral roots and in or close to the shoot phloem. The spatiotemporal expression pattern of NRT2.4 in roots is complementary with that of the major high-affinity nitrate transporter NTR2.1. Functional analysis in Xenopus laevis oocytes and in planta showed that NRT2.4 is a nitrate transporter functioning in the high-affinity range. In N-starved nrt2.4 mutants, nitrate uptake under low external supply and nitrate content in shoot phloem exudates was decreased. In the absence of NRT2.1 and NRT2.2, loss of function of NRT2.4 (triple mutants) has an impact on biomass production under low nitrate supply. Together, our results demonstrate that NRT2.4 is a nitrate transporter that has a role in both roots and shoots under N starvation.
Almagro,
Characterization of the Arabidopsis nitrate transporter NRT1.6 reveals a role of nitrate in early embryo development.
2008, Pubmed,
Xenbase
Almagro,
Characterization of the Arabidopsis nitrate transporter NRT1.6 reveals a role of nitrate in early embryo development.
2008,
Pubmed
,
Xenbase
Alonso,
Genome-wide insertional mutagenesis of Arabidopsis thaliana.
2003,
Pubmed
Arabidopsis Genome Initiative,
Analysis of the genome sequence of the flowering plant Arabidopsis thaliana.
2000,
Pubmed
Behl,
Induction of a high-capacity nitrate-uptake mechanism in barley roots prompted by nitrate uptake through a constitutive low-capacity mechanism.
1988,
Pubmed
Bertoni,
A nitrate transporter for both roots and shoots.
2012,
Pubmed
Brady,
A high-resolution root spatiotemporal map reveals dominant expression patterns.
2007,
Pubmed
Castaings,
The nodule inception-like protein 7 modulates nitrate sensing and metabolism in Arabidopsis.
2009,
Pubmed
Cerezo,
Major alterations of the regulation of root NO(3)(-) uptake are associated with the mutation of Nrt2.1 and Nrt2.2 genes in Arabidopsis.
2001,
Pubmed
Chiu,
Mutation of a nitrate transporter, AtNRT1:4, results in a reduced petiole nitrate content and altered leaf development.
2004,
Pubmed
,
Xenbase
Chopin,
The Arabidopsis ATNRT2.7 nitrate transporter controls nitrate content in seeds.
2007,
Pubmed
,
Xenbase
Cleaver,
Overexpression of the tinman-related genes XNkx-2.5 and XNkx-2.3 in Xenopus embryos results in myocardial hyperplasia.
1996,
Pubmed
,
Xenbase
Clough,
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.
1998,
Pubmed
Daniel-Vedele,
A tobacco cDNA clone encoding a GATA-1 zinc finger protein homologous to regulators of nitrogen metabolism in fungi.
1993,
Pubmed
Daniel-Vedele,
Nitrate transport: a key step in nitrate assimilation.
1998,
Pubmed
De Angeli,
The nitrate/proton antiporter AtCLCa mediates nitrate accumulation in plant vacuoles.
2006,
Pubmed
Dechorgnat,
From the soil to the seeds: the long journey of nitrate in plants.
2011,
Pubmed
Fan,
The Arabidopsis nitrate transporter NRT1.7, expressed in phloem, is responsible for source-to-sink remobilization of nitrate.
2009,
Pubmed
Filleur,
An arabidopsis T-DNA mutant affected in Nrt2 genes is impaired in nitrate uptake.
2001,
Pubmed
Filleur,
Expression analysis of a high-affinity nitrate transporter isolated from Arabidopsis thaliana by differential display.
1999,
Pubmed
Forde,
Nitrate transporters in plants: structure, function and regulation.
2000,
Pubmed
Forsbach,
A comprehensive characterization of single-copy T-DNA insertions in the Arabidopsis thaliana genome.
2003,
Pubmed
Fraisier,
Constitutive expression of a putative high-affinity nitrate transporter in Nicotiana plumbaginifolia: evidence for post-transcriptional regulation by a reduced nitrogen source.
2000,
Pubmed
Galván,
Nitrate and nitrate are transported by different specific transport systems and by a bispecific transporter in Chlamydomonas reinhardtii.
1996,
Pubmed
Gordon-Weeks,
Restricted spatial expression of a high-affinity phosphate transporter in potato roots.
2003,
Pubmed
Haritatos,
Minor vein structure and sugar transport in Arabidopsis thaliana.
2000,
Pubmed
Hellens,
pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation.
2000,
Pubmed
Ho,
CHL1 functions as a nitrate sensor in plants.
2009,
Pubmed
Huang,
Cloning and functional characterization of an Arabidopsis nitrate transporter gene that encodes a constitutive component of low-affinity uptake.
1999,
Pubmed
,
Xenbase
Jackson,
Nitrate Uptake by Dark-grown Corn Seedlings: Some Characteristics of Apparent Induction.
1973,
Pubmed
Jossier,
The Arabidopsis vacuolar anion transporter, AtCLCc, is involved in the regulation of stomatal movements and contributes to salt tolerance.
2010,
Pubmed
Kiba,
Targeted degradation of PSEUDO-RESPONSE REGULATOR5 by an SCFZTL complex regulates clock function and photomorphogenesis in Arabidopsis thaliana.
2007,
Pubmed
Krouk,
Nitrate-regulated auxin transport by NRT1.1 defines a mechanism for nutrient sensing in plants.
2010,
Pubmed
,
Xenbase
Lejay,
Molecular and functional regulation of two NO3- uptake systems by N- and C-status of Arabidopsis plants.
1999,
Pubmed
Li,
Dissection of the AtNRT2.1:AtNRT2.2 inducible high-affinity nitrate transporter gene cluster.
2007,
Pubmed
Li,
The Arabidopsis nitrate transporter NRT1.8 functions in nitrate removal from the xylem sap and mediates cadmium tolerance.
2010,
Pubmed
,
Xenbase
Lin,
Mutation of the Arabidopsis NRT1.5 nitrate transporter causes defective root-to-shoot nitrate transport.
2008,
Pubmed
,
Xenbase
Little,
The putative high-affinity nitrate transporter NRT2.1 represses lateral root initiation in response to nutritional cues.
2005,
Pubmed
Liu,
CHL1 is a dual-affinity nitrate transporter of Arabidopsis involved in multiple phases of nitrate uptake.
1999,
Pubmed
,
Xenbase
Liu,
Switching between the two action modes of the dual-affinity nitrate transporter CHL1 by phosphorylation.
2003,
Pubmed
,
Xenbase
Ma,
A silicon transporter in rice.
2006,
Pubmed
Ma,
An efflux transporter of silicon in rice.
2007,
Pubmed
Masclaux-Daubresse,
Leaf nitrogen remobilisation for plant development and grain filling.
2008,
Pubmed
Mickelson,
Mapping of QTL associated with nitrogen storage and remobilization in barley (Hordeum vulgare L.) leaves.
2003,
Pubmed
Miranda,
A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite.
2001,
Pubmed
Naito,
Expression of a Soybean (Glycine max [L.] Merr.) Seed Storage Protein Gene in Transgenic Arabidopsis thaliana and Its Response to Nutritional Stress and to Abscisic Acid Mutations.
1994,
Pubmed
Nazoa,
Regulation of the nitrate transporter gene AtNRT2.1 in Arabidopsis thaliana: responses to nitrate, amino acids and developmental stage.
2003,
Pubmed
Okamoto,
Regulation of NRT1 and NRT2 gene families of Arabidopsis thaliana: responses to nitrate provision.
2003,
Pubmed
Okamoto,
High-affinity nitrate transport in roots of Arabidopsis depends on expression of the NAR2-like gene AtNRT3.1.
2006,
Pubmed
Orsel,
Characterization of a two-component high-affinity nitrate uptake system in Arabidopsis. Physiology and protein-protein interaction.
2006,
Pubmed
,
Xenbase
Orsel,
Disruption of the nitrate transporter genes AtNRT2.1 and AtNRT2.2 restricts growth at low external nitrate concentration.
2004,
Pubmed
Orsel,
Analysis of the NRT2 nitrate transporter family in Arabidopsis. Structure and gene expression.
2002,
Pubmed
Sasaki,
Detection of several mRNA species in rice phloem sap.
1998,
Pubmed
Segonzac,
Nitrate efflux at the root plasma membrane: identification of an Arabidopsis excretion transporter.
2007,
Pubmed
Smith,
Osmoregulation and the control of phloem-sap composition in Ricinus communis L.
1980,
Pubmed
Sylvester-Bradley,
Analysing nitrogen responses of cereals to prioritize routes to the improvement of nitrogen use efficiency.
2009,
Pubmed
Takano,
Polar localization and degradation of Arabidopsis boron transporters through distinct trafficking pathways.
2010,
Pubmed
Tilman,
Forecasting agriculturally driven global environmental change.
2001,
Pubmed
Tong,
A two-component high-affinity nitrate uptake system in barley.
2005,
Pubmed
,
Xenbase
Töpfer,
A set of plant expression vectors for transcriptional and translational fusions.
1987,
Pubmed
Truernit,
High-resolution whole-mount imaging of three-dimensional tissue organization and gene expression enables the study of Phloem development and structure in Arabidopsis.
2008,
Pubmed
Tsay,
The herbicide sensitivity gene CHL1 of Arabidopsis encodes a nitrate-inducible nitrate transporter.
1993,
Pubmed
,
Xenbase
von der Fecht-Bartenbach,
CLC-b-mediated NO-3/H+ exchange across the tonoplast of Arabidopsis vacuoles.
2010,
Pubmed
,
Xenbase
Wang,
Arabidopsis nitrate transporter NRT1.9 is important in phloem nitrate transport.
2011,
Pubmed
,
Xenbase
Wang,
The Arabidopsis CHL1 protein plays a major role in high-affinity nitrate uptake.
1998,
Pubmed
Wirth,
Regulation of root nitrate uptake at the NRT2.1 protein level in Arabidopsis thaliana.
2007,
Pubmed
Yong,
Characterization of an intact two-component high-affinity nitrate transporter from Arabidopsis roots.
2010,
Pubmed
Yuan,
The organization of high-affinity ammonium uptake in Arabidopsis roots depends on the spatial arrangement and biochemical properties of AMT1-type transporters.
2007,
Pubmed
Zhang,
Global characterization of cell-specific gene expression through fluorescence-activated sorting of nuclei.
2008,
Pubmed
Zifarelli,
Conversion of the 2 Cl(-)/1 H+ antiporter ClC-5 in a NO3(-)/H+ antiporter by a single point mutation.
2009,
Pubmed
,
Xenbase