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1. Myocyte-dependent regulation of acetylcholine (ACh) quantal secretion from developing motoneurons was studied in day-3 Xenopus nerve-muscle co-cultures. Spontaneous synaptic currents (SSCs) were measured in manipulated synapses by using whole-cell voltage-clamped myocytes. Changes in SSC amplitude were assumed to reflect changes in the ACh content of secreted quantal packets. Compared with natural synapses, motoneurons without any contact with a myocyte (naive neurons) released ACh in smaller quantal packets. 2. Bipolar cultured motoneurons, which were in contact with a myocyte with one axon branch (contact-end) but remained free at another axon branch (free-end), were further used to examine quantal ACh secretion. The ACh quantal size recorded at free-end terminals was similar to that of naive neurons and was smaller than that at the contact-end, indicating that myocyte contact exerts differential regulation on quantal secretion in the same neuron. 3. Some of the neurons that formed a natural synapse with a myocyte continued to grow forward and ACh quantal secretion from the free growth cone was examined. The ACh quantal size recorded at free growth cones was inversely proportional to the distance to the natural synapse, implying localized regulation of quantal secretion by the myocyte. 4. Chronic treatment of day-1 cultures with veratridine and d-tubocurarine, respectively, increased and decreased the neurotrophic action of myocytes when assayed on day 3. 5. Taken together, these findings suggest that the myocyte is an important postsynaptic target in the regulation of quantal secretion and that the trophic action is spatially restricted to the neighbourhood of the neuromuscular junction.
Balice-Gordon,
In vivo observations of pre- and postsynaptic changes during the transition from multiple to single innervation at developing neuromuscular junctions.
1993, Pubmed
Balice-Gordon,
In vivo observations of pre- and postsynaptic changes during the transition from multiple to single innervation at developing neuromuscular junctions.
1993,
Pubmed
Bear,
Synaptic plasticity: LTP and LTD.
1994,
Pubmed
Campenot,
Local control of neurite sprouting in cultured sympathetic neurons by nerve growth factor.
1987,
Pubmed
Campenot,
Development of sympathetic neurons in compartmentalized cultures. Il Local control of neurite growth by nerve growth factor.
1982,
Pubmed
Campenot,
NGF and the local control of nerve terminal growth.
1994,
Pubmed
Caroni,
Activity-sensitive signaling by muscle-derived insulin-like growth factors in the developing and regenerating neuromuscular system.
1993,
Pubmed
Connor,
Retrograde signaling in the formation and maintenance of the neuromuscular junction.
1994,
Pubmed
Dai,
Elevation in presynaptic Ca2+ level accompanying initial nerve-muscle contact in tissue culture.
1993,
Pubmed
,
Xenbase
Dan,
Retrograde interactions during formation and elimination of neuromuscular synapses.
1994,
Pubmed
Eftimie,
Myogenin and MyoD join a family of skeletal muscle genes regulated by electrical activity.
1991,
Pubmed
Evers,
Studies of nerve-muscle interactions in Xenopus cell culture: analysis of early synaptic currents.
1989,
Pubmed
,
Xenbase
Fu,
Regulatory role of ATP at developing neuromuscular junctions.
1995,
Pubmed
,
Xenbase
Goldman,
Acetylcholine receptor alpha-, beta-, gamma-, and delta-subunit mRNA levels are regulated by muscle activity.
1988,
Pubmed
Hall,
Synaptic structure and development: the neuromuscular junction.
1993,
Pubmed
Hamburger,
Cell death in the development of the lateral motor column of the chick embryo.
1975,
Pubmed
Hamill,
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1981,
Pubmed
Henderson,
GDNF: a potent survival factor for motoneurons present in peripheral nerve and muscle.
1994,
Pubmed
Henderson,
Neurotrophins promote motor neuron survival and are present in embryonic limb bud.
1993,
Pubmed
Ikeda,
Basic fibroblast growth factor has neuroprotective effects on axotomy-induced spinal motoneuron death and wobbler mouse motoneuron disease.
1996,
Pubmed
Isackson,
Trophic factor response to neuronal stimuli or injury.
1995,
Pubmed
Jennings,
Development of the neuromuscular synapse.
1993,
Pubmed
Kidokoro,
Early cross-striation formation in twitching Xenopus myocytes in culture.
1988,
Pubmed
,
Xenbase
Korsching,
The neurotrophic factor concept: a reexamination.
1993,
Pubmed
Liou,
Regulation of quantal secretion from developing motoneurons by postsynaptic activity-dependent release of NT-3.
1997,
Pubmed
,
Xenbase
Liou,
Regulation of quantal secretion by neurotrophic factors at developing motoneurons in Xenopus cell cultures.
1997,
Pubmed
,
Xenbase
Lohof,
Potentiation of developing neuromuscular synapses by the neurotrophins NT-3 and BDNF.
1993,
Pubmed
,
Xenbase
Mason,
Release of the predicted calcitonin gene-related peptide from cultured rat trigeminal ganglion cells.
,
Pubmed
McMahan,
Molecules in basal lamina that direct the formation of synaptic specializations at neuromuscular junctions.
1989,
Pubmed
McManaman,
Rescue of motoneurons from cell death by a purified skeletal muscle polypeptide: effects of the ChAT development factor, CDF.
1990,
Pubmed
Northcutt,
Body and Brain. A Trophic Theory of Neural Connections. Dale Purves. Harvard University Press, Cambridge, MA, 1988. viii, 231 pp., illus. $35.
1989,
Pubmed
Reist,
Agrin released by motor neurons induces the aggregation of acetylcholine receptors at neuromuscular junctions.
1992,
Pubmed
Sendtner,
Synthesis and localization of ciliary neurotrophic factor in the sciatic nerve of the adult rat after lesion and during regeneration.
1992,
Pubmed
Shigemoto,
Target-cell-specific concentration of a metabotropic glutamate receptor in the presynaptic active zone.
1996,
Pubmed
Stoop,
Synaptic modulation by neurotrophic factors: differential and synergistic effects of brain-derived neurotrophic factor and ciliary neurotrophic factor.
1996,
Pubmed
,
Xenbase
Takahashi,
Structure and physiology of developing neuromuscular synapses in culture.
1987,
Pubmed
,
Xenbase
Wang,
Neurotrophins promote maturation of developing neuromuscular synapses.
1995,
Pubmed
,
Xenbase
Wang,
Localized synaptic actions of neurotrophin-4.
1998,
Pubmed
,
Xenbase
Xie,
Initial events in the formation of neuromuscular synapse: rapid induction of acetylcholine release from embryonic neuron.
1986,
Pubmed
,
Xenbase
Zafra,
Regulation of brain-derived neurotrophic factor and nerve growth factor mRNA in primary cultures of hippocampal neurons and astrocytes.
1992,
Pubmed
