Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
The expanding roles of telomeres in epigenetic gene regulation, nuclear organization, and human disease have necessitated the establishment of model organisms in which to study telomere function under normal developmental conditions. We present an efficient system for generating numerous vertebrate animals containing engineered telomeres using a Xenopus laevis transgenesis technique. Our results indicate Xenopus zygotes efficiently recognize telomeric repeats at chromosome break points and form telomeric complexes thus generating a new telomere. The resulting transgenic animals progress through normal development and successfully metamorphose into froglets despite the chromosome breakage. Overall, this presents an efficient mechanism for generating engineered telomeres in a vertebrate system and provides an opportunity to investigate epigenetic aspects of telomere function during normal vertebrate development.
Amaya,
Xenomics.
2005,
Pubmed
,
Xenbase
Bailey,
Telomeres, chromosome instability and cancer.
2006,
Pubmed
Baird,
Extensive allelic variation and ultrashort telomeres in senescent human cells.
2003,
Pubmed
Bassham,
Telomere variation in Xenopus laevis.
1998,
Pubmed
,
Xenbase
Baur,
Telomere position effect in human cells.
2001,
Pubmed
Baur,
Analysis of mammalian telomere position effect.
2004,
Pubmed
Bertuch,
The maintenance and masking of chromosome termini.
2006,
Pubmed
Blackburn,
Telomeres and telomerase: their mechanisms of action and the effects of altering their functions.
2005,
Pubmed
Bousman,
Telomerase activity is widespread in adult somatic tissues of Xenopus.
2003,
Pubmed
,
Xenbase
de Lange,
Activation of telomerase in a human tumor.
1994,
Pubmed
Förstemann,
Molecular basis for telomere repeat divergence in budding yeast.
2001,
Pubmed
Gehring,
Functional analysis of the white gene of Drosophila by P-factor-mediated transformation.
1984,
Pubmed
Gotta,
Nuclear organization and transcriptional silencing in yeast.
1996,
Pubmed
Gottschling,
Position effect at S. cerevisiae telomeres: reversible repression of Pol II transcription.
1990,
Pubmed
Greider,
Telomeres, telomerase and senescence.
1990,
Pubmed
Hanish,
Stringent sequence requirements for the formation of human telomeres.
1994,
Pubmed
Harley,
Telomeres shorten during ageing of human fibroblasts.
1990,
Pubmed
Hastie,
Telomere reduction in human colorectal carcinoma and with ageing.
1990,
Pubmed
Hazelrigg,
Transformation of white locus DNA in drosophila: dosage compensation, zeste interaction, and position effects.
1984,
Pubmed
Heasman,
Patterning the early Xenopus embryo.
2006,
Pubmed
,
Xenbase
Henderson,
An overhanging 3' terminus is a conserved feature of telomeres.
1989,
Pubmed
Khokha,
Techniques and probes for the study of Xenopus tropicalis development.
2002,
Pubmed
,
Xenbase
Kim,
Specific association of human telomerase activity with immortal cells and cancer.
1994,
Pubmed
Kroll,
Transgenic Xenopus embryos from sperm nuclear transplantations reveal FGF signaling requirements during gastrulation.
1996,
Pubmed
,
Xenbase
Kyrion,
RAP1 and telomere structure regulate telomere position effects in Saccharomyces cerevisiae.
1993,
Pubmed
Levis,
Transposons in place of telomeric repeats at a Drosophila telomere.
1993,
Pubmed
Levy,
Telomere end-replication problem and cell aging.
1992,
Pubmed
Lohr,
Vertebrate model systems in the study of early heart development: Xenopus and zebrafish.
2000,
Pubmed
,
Xenbase
Mantell,
Telomerase activity in germline and embryonic cells of Xenopus.
1994,
Pubmed
,
Xenbase
McClintock,
The Stability of Broken Ends of Chromosomes in Zea Mays.
1941,
Pubmed
Meyne,
Distribution of non-telomeric sites of the (TTAGGG)n telomeric sequence in vertebrate chromosomes.
1990,
Pubmed
Meyne,
Conservation of the human telomere sequence (TTAGGG)n among vertebrates.
1989,
Pubmed
Moyzis,
A highly conserved repetitive DNA sequence, (TTAGGG)n, present at the telomeres of human chromosomes.
1988,
Pubmed
Nimmo,
Telomere-associated chromosome breakage in fission yeast results in variegated expression of adjacent genes.
1994,
Pubmed
Pedram,
Telomere position effect and silencing of transgenes near telomeres in the mouse.
2006,
Pubmed
Tam,
The 4q subtelomere harboring the FSHD locus is specifically anchored with peripheral heterochromatin unlike most human telomeres.
2004,
Pubmed
van der Maarel,
Facioscapulohumeral muscular dystrophy.
2007,
Pubmed
