Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Cell Res
2012 Nov 01;2211:1562-75. doi: 10.1038/cr.2012.113.
Show Gene links
Show Anatomy links
Chromatin-bound NLS proteins recruit membrane vesicles and nucleoporins for nuclear envelope assembly via importin-α/β.
Lu Q
,
Lu Z
,
Liu Q
,
Guo L
,
Ren H
,
Fu J
,
Jiang Q
,
Clarke PR
,
Zhang C
.
???displayArticle.abstract???
The mechanism for nuclear envelope (NE) assembly is not fully understood. Importin-β and the small GTPase Ran have been implicated in the spatial regulation of NE assembly process. Here we report that chromatin-bound NLS (nuclear localization sequence) proteins provide docking sites for the NE precursor membrane vesicles and nucleoporins via importin-α and -β during NE assembly in Xenopus egg extracts. We show that along with the fast recruitment of the abundant NLS proteins such as nucleoplasmin and histones to the demembranated sperm chromatin in the extracts, importin-α binds the chromatin NLS proteins rapidly. Meanwhile, importin-β binds cytoplasmic NE precursor membrane vesicles and nucleoporins. Through interacting with importin-α on the chromatin NLS proteins, importin-β targets the membrane vesicles and nucleoporins to the chromatin surface. Once encountering Ran-GTP on the chromatin generated by RCC1, importin-β preferentially binds Ran-GTP and releases the membrane vesicles and nucleoporins for NE assembly. NE assembly is disrupted by blocking the interaction between importin-α and NLS proteins with excess soluble NLS proteins or by depletion of importin-β from the extract. Our findings reveal a novel molecular mechanism for NE assembly in Xenopus egg extracts.
???displayArticle.pubmedLink???
22847741
???displayArticle.pmcLink???PMC3494395 ???displayArticle.link???Cell Res ???displayArticle.grants???[+]
Antonin,
The integral membrane nucleoporin pom121 functionally links nuclear pore complex assembly and nuclear envelope formation.
2005, Pubmed,
Xenbase
Antonin,
The integral membrane nucleoporin pom121 functionally links nuclear pore complex assembly and nuclear envelope formation.
2005,
Pubmed
,
Xenbase
Bañuelos,
Activation mechanism of the nuclear chaperone nucleoplasmin: role of the core domain.
2003,
Pubmed
,
Xenbase
Bilbao-Cortés,
Ran binds to chromatin by two distinct mechanisms.
2002,
Pubmed
,
Xenbase
Clarke,
Spatial and temporal control of nuclear envelope assembly by Ran GTPase.
2004,
Pubmed
,
Xenbase
Clarke,
Spatial and temporal coordination of mitosis by Ran GTPase.
2008,
Pubmed
Dingwall,
Nucleoplasmin cDNA sequence reveals polyglutamic acid tracts and a cluster of sequences homologous to putative nuclear localization signals.
1987,
Pubmed
,
Xenbase
Dingwall,
A polypeptide domain that specifies migration of nucleoplasmin into the nucleus.
1982,
Pubmed
,
Xenbase
Fernandez,
MEL-28 is downstream of the Ran cycle and is required for nuclear-envelope function and chromatin maintenance.
2006,
Pubmed
Fichtman,
Inner/Outer nuclear membrane fusion in nuclear pore assembly: biochemical demonstration and molecular analysis.
2010,
Pubmed
,
Xenbase
Franz,
MEL-28/ELYS is required for the recruitment of nucleoporins to chromatin and postmitotic nuclear pore complex assembly.
2007,
Pubmed
,
Xenbase
Gant,
Nuclear assembly.
1997,
Pubmed
Gillespie,
ELYS/MEL-28 chromatin association coordinates nuclear pore complex assembly and replication licensing.
2007,
Pubmed
,
Xenbase
Goldfarb,
Importin alpha: a multipurpose nuclear-transport receptor.
2004,
Pubmed
Görlich,
Distinct functions for the two importin subunits in nuclear protein import.
1995,
Pubmed
,
Xenbase
Gruss,
Ran induces spindle assembly by reversing the inhibitory effect of importin alpha on TPX2 activity.
2001,
Pubmed
,
Xenbase
Hachet,
Importin alpha associates with membranes and participates in nuclear envelope assembly in vitro.
2004,
Pubmed
,
Xenbase
Harel,
Importin beta negatively regulates nuclear membrane fusion and nuclear pore complex assembly.
2003,
Pubmed
,
Xenbase
Hartl,
Nuclear assembly with lambda DNA in fractionated Xenopus egg extracts: an unexpected role for glycogen in formation of a higher order chromatin intermediate.
1994,
Pubmed
,
Xenbase
Hetzer,
GTP hydrolysis by Ran is required for nuclear envelope assembly.
2000,
Pubmed
,
Xenbase
Hetzer,
Border control at the nucleus: biogenesis and organization of the nuclear membrane and pore complexes.
2009,
Pubmed
Hierro,
Electrostatic interactions at the C-terminal domain of nucleoplasmin modulate its chromatin decondensation activity.
2002,
Pubmed
,
Xenbase
Hughes,
The role of the ran GTPase in nuclear assembly and DNA replication: characterisation of the effects of Ran mutants.
1998,
Pubmed
,
Xenbase
Hutchins,
Dynamic localisation of Ran GTPase during the cell cycle.
2009,
Pubmed
Laskey,
The role of nucleoplasmin in chromatin assembly and disassembly.
1993,
Pubmed
,
Xenbase
Liu,
Pericentrin contains five NESs and an NLS essential for its nucleocytoplasmic trafficking during the cell cycle.
2010,
Pubmed
Lu,
Nucleoplasmin regulates chromatin condensation during apoptosis.
2005,
Pubmed
,
Xenbase
Lu,
Requirement for lamin B receptor and its regulation by importin {beta} and phosphorylation in nuclear envelope assembly during mitotic exit.
2010,
Pubmed
,
Xenbase
Ma,
Lamin B receptor plays a role in stimulating nuclear envelope production and targeting membrane vesicles to chromatin during nuclear envelope assembly through direct interaction with importin beta.
2007,
Pubmed
,
Xenbase
Merdes,
A complex of NuMA and cytoplasmic dynein is essential for mitotic spindle assembly.
1996,
Pubmed
,
Xenbase
Mitchell,
Pom121 links two essential subcomplexes of the nuclear pore complex core to the membrane.
2010,
Pubmed
Philpott,
Nucleoplasmin remodels sperm chromatin in Xenopus egg extracts.
1992,
Pubmed
,
Xenbase
Philpott,
Sperm decondensation in Xenopus egg cytoplasm is mediated by nucleoplasmin.
1991,
Pubmed
,
Xenbase
Rasala,
Capture of AT-rich chromatin by ELYS recruits POM121 and NDC1 to initiate nuclear pore assembly.
2008,
Pubmed
,
Xenbase
Rasala,
ELYS is a dual nucleoporin/kinetochore protein required for nuclear pore assembly and proper cell division.
2006,
Pubmed
,
Xenbase
Rice,
Nucleoplasmin-mediated decondensation of Mytilus sperm chromatin. Identification and partial characterization of a nucleoplasmin-like protein with sperm-nuclei decondensing activity in Mytilus californianus.
1995,
Pubmed
,
Xenbase
Ryan,
The Ran GTPase cycle is required for yeast nuclear pore complex assembly.
2003,
Pubmed
Vasu,
Nuclear pores and nuclear assembly.
2001,
Pubmed
Walther,
RanGTP mediates nuclear pore complex assembly.
2003,
Pubmed
,
Xenbase
Walther,
The conserved Nup107-160 complex is critical for nuclear pore complex assembly.
2003,
Pubmed
,
Xenbase
Wiese,
Role of importin-beta in coupling Ran to downstream targets in microtubule assembly.
2001,
Pubmed
,
Xenbase
Yang,
NuMA: an unusually long coiled-coil related protein in the mammalian nucleus.
1992,
Pubmed
Zhang,
Roles of Ran-GTP and Ran-GDP in precursor vesicle recruitment and fusion during nuclear envelope assembly in a human cell-free system.
2001,
Pubmed
,
Xenbase
Zhang,
Role of importin-beta in the control of nuclear envelope assembly by Ran.
2002,
Pubmed
,
Xenbase
Zhang,
Concentration of Ran on chromatin induces decondensation, nuclear envelope formation and nuclear pore complex assembly.
2002,
Pubmed
,
Xenbase
Zhang,
Chromatin-independent nuclear envelope assembly induced by Ran GTPase in Xenopus egg extracts.
2000,
Pubmed
,
Xenbase