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Transport and turnover of microtubules in frog neurons depend on the pattern of axonal growth.
Chang S
,
Rodionov VI
,
Borisy GG
,
Popov SV
.
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The transport of axonal microtubules in growing neurites has been a controversial issue because of clear but conflicting results obtained with fluorescence-marking techniques. We have attempted to resolve the discordance via analysis of the relationship between apparent microtubule translocation and cell adhesion. Neuronal cultures were prepared from Xenopus embryos 1 d after injection of Cy3-conjugated tubulin into one of the blastomeres of two-cell-stage embryos. Anterograde translocation of axonal microtubules was observed in neurons cultured on a laminin-coated surface, in agreement with previously published data for Xenopus embryonic neurons. However, when neuronal cultures were prepared on a concanavalin A-treated surface, the axonal microtubules were stationary, as reported for all other neurons investigated previously. Neuronal cultures prepared on laminin- and concanavalin A-coated surfaces also demonstrated dramatic differences in the pattern of axonal growth, dynamics of axonal microtubules, and response to brefeldin A treatment. Our findings suggest that transport and dynamics of axonal microtubules may be directly affected by the mechanical tension produced by growth cone activity.
Ahmad,
Microtubules released from the neuronal centrosome are transported into the axon.
1995, Pubmed
Ahmad,
Microtubules released from the neuronal centrosome are transported into the axon.
1995,
Pubmed
Baas,
Microtubules and axonal growth.
1997,
Pubmed
Baas,
Gamma-tubulin distribution in the neuron: implications for the origins of neuritic microtubules.
1992,
Pubmed
Baas,
The transport properties of axonal microtubules establish their polarity orientation.
1993,
Pubmed
Bamburg,
Assembly of microtubules at the tip of growing axons.
,
Pubmed
Black,
Slow components of axonal transport: two cytoskeletal networks.
1980,
Pubmed
Borisy,
Purification of tubulin and associated high molecular weight proteins from porcine brain and characterization of microtubule assembly in vitro.
1975,
Pubmed
Bray,
Axonal growth in response to experimentally applied mechanical tension.
1984,
Pubmed
Craig,
Preferential addition of newly synthesized membrane protein at axonal growth cones.
1995,
Pubmed
Dai,
Axon membrane flows from the growth cone to the cell body.
1995,
Pubmed
Edson,
FRAP analysis of the stability of the microtubule population along the neurites of chick sensory neurons.
1993,
Pubmed
Funakoshi,
Active transport of photoactivated tubulin molecules in growing axons revealed by a new electron microscopic analysis.
1996,
Pubmed
Futerman,
The economics of neurite outgrowth--the addition of new membrane to growing axons.
1996,
Pubmed
Gorbsky,
Chromosomes move poleward in anaphase along stationary microtubules that coordinately disassemble from their kinetochore ends.
1987,
Pubmed
Hoffman,
The slow component of axonal transport. Identification of major structural polypeptides of the axon and their generality among mammalian neurons.
1975,
Pubmed
Ingber,
Tensegrity: the architectural basis of cellular mechanotransduction.
1997,
Pubmed
Jacobson,
Neurite outgrowth traced by means of horseradish peroxidase inherited from neuronal ancestral cells in frog embryos.
1985,
Pubmed
,
Xenbase
Keating,
Microtubule release from the centrosome.
1997,
Pubmed
Lamoureux,
Direct evidence that growth cones pull.
1989,
Pubmed
Li,
Microtubule assembly and turnover in growing axons.
1996,
Pubmed
Lim,
A test of microtubule translocation during neurite elongation.
1990,
Pubmed
Lippincott-Schwartz,
Microtubule-dependent retrograde transport of proteins into the ER in the presence of brefeldin A suggests an ER recycling pathway.
1990,
Pubmed
Lippincott-Schwartz,
Rapid redistribution of Golgi proteins into the ER in cells treated with brefeldin A: evidence for membrane cycling from Golgi to ER.
1989,
Pubmed
Lohof,
Potentiation of developing neuromuscular synapses by the neurotrophins NT-3 and BDNF.
1993,
Pubmed
,
Xenbase
Maniotis,
Demonstration of mechanical connections between integrins, cytoskeletal filaments, and nucleoplasm that stabilize nuclear structure.
1997,
Pubmed
Martenson,
Fast axonal transport is required for growth cone advance.
1993,
Pubmed
Miller,
Tubulin transport in neurons.
1996,
Pubmed
Moritz,
Microtubule nucleation by gamma-tubulin-containing rings in the centrosome.
1995,
Pubmed
Okabe,
Differential behavior of photoactivated microtubules in growing axons of mouse and frog neurons.
1992,
Pubmed
,
Xenbase
Okabe,
Turnover of fluorescently labelled tubulin and actin in the axon.
1990,
Pubmed
Okabe,
Do photobleached fluorescent microtubules move?: re-evaluation of fluorescence laser photobleaching both in vitro and in growing Xenopus axon.
1993,
Pubmed
,
Xenbase
Popov,
Forward plasma membrane flow in growing nerve processes.
1993,
Pubmed
,
Xenbase
Popov,
Diffusional transport of macromolecules in developing nerve processes.
1992,
Pubmed
,
Xenbase
Reinsch,
Microtubule polymer assembly and transport during axonal elongation.
1991,
Pubmed
,
Xenbase
Rodionov,
Microtubule dynamics in fish melanophores.
1994,
Pubmed
Sabry,
Axonal transport of tubulin in Ti1 pioneer neurons in situ.
1995,
Pubmed
Slaughter,
Microtubule transport from the cell body into the axons of growing neurons.
1997,
Pubmed
Spitzer,
The development of the action potential mechanism of amphibian neurons isolated in culture.
1976,
Pubmed
,
Xenbase
Stoop,
Potentiation of transmitter release by ciliary neurotrophic factor requires somatic signaling.
1995,
Pubmed
,
Xenbase
Takeda,
Tubulin dynamics in neuronal axons of living zebrafish embryos.
1995,
Pubmed
Tanaka,
The role of microtubule dynamics in growth cone motility and axonal growth.
1995,
Pubmed
,
Xenbase
Wang,
Neurotrophins promote maturation of developing neuromuscular synapses.
1995,
Pubmed
,
Xenbase
Zheng,
Nucleation of microtubule assembly by a gamma-tubulin-containing ring complex.
1995,
Pubmed
,
Xenbase
Zheng,
Investigation of microtubule assembly and organization accompanying tension-induced neurite initiation.
1993,
Pubmed
Zheng,
Tensile regulation of axonal elongation and initiation.
1991,
Pubmed
