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Mol Cell Biol
1989 Dec 01;912:5281-8. doi: 10.1128/mcb.9.12.5281-5288.1989.
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Developmental expression and 5S rRNA-binding activity of Xenopus laevis ribosomal protein L5.
Wormington WM
.
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Ribosomal protein L5 binds specifically to 5S rRNA to form a complex that is a precursor to 60S subunit assembly in vivo. Analyses in yeast cells, mammalian cells, and Xenopus embryos have shown that the accumulation of L5 is not coordinated with the expression of other ribosomal proteins. In this study, the primary structure and developmental expression of Xenopus ribosomal protein L5 were examined to determine the basis for its distinct regulation. These analyses showed that L5 expression could either coincide with 5S rRNA synthesis and ribosome assembly or be controlled independently of these events at different stages of Xenopus development. L5 synthesis during oogenesis was uncoupled from the accumulation of 5S rRNa but coincided with subunit assembly. In early embryos, the inefficient translation of L5 mRNA resulted in the accumulation of a stable L5-5S rRNA complex before ribosome assembly at later stages of development. Additional results demonstrated that L5 protein synthesized in vitro bound specifically to 5S rRNA.
Bandziulis,
RNA-binding proteins as developmental regulators.
1989, Pubmed
Bandziulis,
RNA-binding proteins as developmental regulators.
1989,
Pubmed
Baum,
Post-translational control of ribosomal protein L1 accumulation in Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Baum,
Coordinate expression of ribosomal protein genes during Xenopus development.
1985,
Pubmed
,
Xenbase
Bisbee,
Albumin phylogeny for clawed frogs (Xenopus).
1977,
Pubmed
,
Xenbase
Brow,
Modulation of yeast 5 S rRNA synthesis in vitro by ribosomal protein YL3. A possible regulatory loop.
1987,
Pubmed
Brown,
Synthesis and accumulation of low molecular weight RNA during embryogenesis of Xenopus laevis.
1966,
Pubmed
,
Xenbase
BROWN,
RNA SYNTHESIS DURING THE DEVELOPMENT OF XENOPUS LAEVIS, THE SOUTH AFRICAN CLAWED TOAD.
1964,
Pubmed
,
Xenbase
Caizergues-Ferrer,
Nucleolin from Xenopus laevis: cDNA cloning and expression during development.
1989,
Pubmed
,
Xenbase
Cardinali,
Ribosomal protein, histone and calmodulin mRNAs are differently regulated at the translational level during oogenesis of Xenopus laevis.
1987,
Pubmed
,
Xenbase
Chan,
The primary structure of rat ribosomal protein L5. A comparison of the sequence of amino acids in the proteins that interact with 5 S rRNA.
1987,
Pubmed
,
Xenbase
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
England,
Specific labeling of 3' termini of RNA with T4 RNA ligase.
1980,
Pubmed
Ginsberg,
Xenopus 5S gene transcription factor, TFIIIA: characterization of a cDNA clone and measurement of RNA levels throughout development.
1984,
Pubmed
,
Xenbase
Gorenstein,
Coordinate regulation of the synthesis of eukaryotic ribosomal proteins.
1976,
Pubmed
Gurdon,
Injected nuclei in frog oocytes: fate, enlargement, and chromatin dispersal.
1976,
Pubmed
,
Xenbase
Huber,
Identification of the binding site on 5S rRNA for the transcription factor IIIA: proposed structure of a common binding site on 5S rRNA and on the gene.
1986,
Pubmed
,
Xenbase
Huber,
Nuclease protection analysis of ribonucleoprotein complexes: use of the cytotoxic ribonuclease alpha-sarcin to determine the binding sites for Escherichia coli ribosomal proteins L5, L18, and L25 on 5S rRNA.
1984,
Pubmed
Huber,
Use of the cytotoxic nuclease alpha-sarcin to identify the binding site on eukaryotic 5 S ribosomal ribonucleic acid for the ribosomal protein L5.
1986,
Pubmed
,
Xenbase
Hyman,
Translational inactivation of ribosomal protein mRNAs during Xenopus oocyte maturation.
1988,
Pubmed
,
Xenbase
Kintner,
Expression of Xenopus N-CAM RNA in ectoderm is an early response to neural induction.
1987,
Pubmed
,
Xenbase
Krieg,
The mRNA encoding elongation factor 1-alpha (EF-1 alpha) is a major transcript at the midblastula transition in Xenopus.
1989,
Pubmed
,
Xenbase
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lastick,
Exchange and stability of HeLa ribosomal proteins in vivo.
1976,
Pubmed
Lerner,
Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosus.
1979,
Pubmed
Loreni,
Nucleotide sequence of the L1 ribosomal protein gene of Xenopus laevis: remarkable sequence homology among introns.
1985,
Pubmed
,
Xenbase
Mager,
Control of ribosomal protein gene expression.
1988,
Pubmed
Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed
Miller,
Metabolism of 5S RNA in the absence of ribosome production.
1974,
Pubmed
,
Xenbase
Nazar,
The 5-S RNA binding protein from yeast (Saccharomyces cerevisiae) ribosomes. Evolution of the eukaryotic 5-S RNA binding protein.
1979,
Pubmed
Pelham,
A specific transcription factor that can bind either the 5S RNA gene or 5S RNA.
1980,
Pubmed
,
Xenbase
Phillips,
Relative stoichiometry in ribosomal proteins in HeLa cell nucleoli.
1976,
Pubmed
Picard,
Isolation of a 7S particle from Xenopus laevis oocytes: a 5S RNA-protein complex.
1979,
Pubmed
,
Xenbase
Pierandrei-Amaldi,
Ribosomal protein production in normal and anucleolate Xenopus embryos: regulation at the posttranscriptional and translational levels.
1985,
Pubmed
,
Xenbase
Pierandrei-Amaldi,
Expression of ribosomal-protein genes in Xenopus laevis development.
1982,
Pubmed
,
Xenbase
Query,
A common RNA recognition motif identified within a defined U1 RNA binding domain of the 70K U1 snRNP protein.
1989,
Pubmed
Romanczuk,
Selective enhancement of bovine papillomavirus type 1 DNA replication in Xenopus laevis eggs by the E6 gene product.
1989,
Pubmed
,
Xenbase
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Steitz,
A 5S rRNA/L5 complex is a precursor to ribosome assembly in mammalian cells.
1988,
Pubmed
Woodland,
Changes in the polysome content of developing Xenopus laevis embryos.
1974,
Pubmed
,
Xenbase
Wormington,
Stable repression of ribosomal protein L1 synthesis in Xenopus oocytes by microinjection of antisense RNA.
1986,
Pubmed
,
Xenbase
Wormington,
Onset of 5 S RNA gene regulation during Xenopus embryogenesis.
1983,
Pubmed
,
Xenbase
Wormington,
Expression of ribosomal protein genes during Xenopus development.
1988,
Pubmed
,
Xenbase
Yaguchi,
The 5S RNA binding protein from yeast (Saccharomyces cerevisiae) ribosomes. An RNA binding sequence in the carboxyl-terminal region.
1984,
Pubmed
