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The Fanconi anemia (FA) pathway proteins are thought to be involved in the repair of irregular DNA structures including those encountered by the moving replication fork. However, the nature of the DNA structures that recruit and activate the FA proteins is not known. Because FA proteins function within an extended network of proteins, some of which are still unknown, we recently established cell-free assays in Xenopus laevis egg extracts to deconstruct the FA pathway in a fully replication-competent context. Here we show that the central FA pathway protein, xFANCD2, is monoubiquitinated (xFANCD2-L) rapidly in the presence of linear and branched double-stranded DNA (dsDNA) structures but not single-stranded or Y-shaped DNA. xFANCD2-L associates with dsDNA structures in an FA core complex-dependent manner but independently of xATRIP, the regulatory subunit of xATR. Formation of xFANCD2-L is also triggered in response to circular dsDNA, suggesting that dsDNA ends are not required to trigger monoubiquitination of FANCD2. The induction of xFANCD2-L in response to circular dsDNA is replication and checkpoint independent. Our results provide new evidence that the FA pathway discriminates among DNA structures and demonstrate that triggering the FA pathway can be uncoupled from DNA replication and ATRIP-dependent activation.
Andreassen,
ATR couples FANCD2 monoubiquitination to the DNA-damage response.
2004, Pubmed
Andreassen,
ATR couples FANCD2 monoubiquitination to the DNA-damage response.
2004,
Pubmed
Aquiles Sanchez,
Efficient plasmid DNA replication in Xenopus egg extracts does not depend on prior chromatin assembly.
1995,
Pubmed
,
Xenbase
Blow,
Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs.
1986,
Pubmed
,
Xenbase
Ciccia,
Identification of FAAP24, a Fanconi anemia core complex protein that interacts with FANCM.
2007,
Pubmed
Conn,
The DNA damage checkpoint in embryonic cell cycles is dependent on the DNA-to-cytoplasmic ratio.
2004,
Pubmed
,
Xenbase
Costanzo,
An ATR- and Cdc7-dependent DNA damage checkpoint that inhibits initiation of DNA replication.
2003,
Pubmed
,
Xenbase
Costanzo,
Mre11 protein complex prevents double-strand break accumulation during chromosomal DNA replication.
2001,
Pubmed
,
Xenbase
Davies,
Role of BRCA2 in control of the RAD51 recombination and DNA repair protein.
2001,
Pubmed
de Winter,
The Fanconi anemia protein FANCF forms a nuclear complex with FANCA, FANCC and FANCG.
2000,
Pubmed
Donahue,
A DNA double strand break repair defect in Fanconi anemia fibroblasts.
2002,
Pubmed
Escarceller,
Fanconi anemia C gene product plays a role in the fidelity of blunt DNA end-joining.
1998,
Pubmed
Garcia-Higuera,
Interaction of the Fanconi anemia proteins and BRCA1 in a common pathway.
2001,
Pubmed
Grompe,
Fanconi anemia and DNA repair.
2001,
Pubmed
Gupta,
Analysis of the DNA substrate specificity of the human BACH1 helicase associated with breast cancer.
2005,
Pubmed
Gutiérrez-Rivas,
A mutation in the primer grip region of HIV-1 reverse transcriptase that confers reduced fidelity of DNA synthesis.
2001,
Pubmed
Ho,
Phosphorylation of FANCD2 on two novel sites is required for mitomycin C resistance.
2006,
Pubmed
Howlett,
Biallelic inactivation of BRCA2 in Fanconi anemia.
2002,
Pubmed
Joenje,
The emerging genetic and molecular basis of Fanconi anaemia.
2001,
Pubmed
Kim,
Phosphorylation of Chk1 by ATM- and Rad3-related (ATR) in Xenopus egg extracts requires binding of ATRIP to ATR but not the stable DNA-binding or coiled-coil domains of ATRIP.
2005,
Pubmed
,
Xenbase
Komori,
Novel endonuclease in Archaea cleaving DNA with various branched structure.
2002,
Pubmed
Komori,
Cooperation of the N-terminal Helicase and C-terminal endonuclease activities of Archaeal Hef protein in processing stalled replication forks.
2004,
Pubmed
Larminat,
Deficiency in BRCA2 leads to increase in non-conservative homologous recombination.
2002,
Pubmed
Levitus,
The DNA helicase BRIP1 is defective in Fanconi anemia complementation group J.
2005,
Pubmed
Levran,
The BRCA1-interacting helicase BRIP1 is deficient in Fanconi anemia.
2005,
Pubmed
Li,
Absence of BLM leads to accumulation of chromosomal DNA breaks during both unperturbed and disrupted S phases.
2004,
Pubmed
,
Xenbase
Lundberg,
Deficient DNA end joining activity in extracts from fanconi anemia fibroblasts.
2001,
Pubmed
Medhurst,
Direct interactions of the five known Fanconi anaemia proteins suggest a common functional pathway.
2001,
Pubmed
Meetei,
X-linked inheritance of Fanconi anemia complementation group B.
2004,
Pubmed
Meetei,
A human ortholog of archaeal DNA repair protein Hef is defective in Fanconi anemia complementation group M.
2005,
Pubmed
Meetei,
A multiprotein nuclear complex connects Fanconi anemia and Bloom syndrome.
2003,
Pubmed
Meetei,
A novel ubiquitin ligase is deficient in Fanconi anemia.
2003,
Pubmed
Mi,
The Fanconi anemia core complex associates with chromatin during S phase.
2005,
Pubmed
Montes de Oca,
Regulated interaction of the Fanconi anemia protein, FANCD2, with chromatin.
2005,
Pubmed
Mosedale,
The vertebrate Hef ortholog is a component of the Fanconi anemia tumor-suppressor pathway.
2005,
Pubmed
Murray,
Cell cycle extracts.
1991,
Pubmed
Nakanishi,
Human Fanconi anemia monoubiquitination pathway promotes homologous DNA repair.
2005,
Pubmed
Niedernhofer,
Fanconi anemia (cross)linked to DNA repair.
2005,
Pubmed
Park,
Direct DNA binding activity of the Fanconi anemia D2 protein.
2005,
Pubmed
Peng,
BACH1 is a DNA repair protein supporting BRCA1 damage response.
2006,
Pubmed
Pichierri,
DNA cross-link-dependent RAD50/MRE11/NBS1 subnuclear assembly requires the Fanconi anemia C protein.
2002,
Pubmed
Qiao,
Fanconi anemia proteins localize to chromatin and the nuclear matrix in a DNA damage- and cell cycle-regulated manner.
2001,
Pubmed
Rahman,
PALB2, which encodes a BRCA2-interacting protein, is a breast cancer susceptibility gene.
2007,
Pubmed
Reid,
Biallelic mutations in PALB2 cause Fanconi anemia subtype FA-N and predispose to childhood cancer.
2007,
Pubmed
Rothfuss,
Repair kinetics of genomic interstrand DNA cross-links: evidence for DNA double-strand break-dependent activation of the Fanconi anemia/BRCA pathway.
2004,
Pubmed
Sekiguchi,
Resolution of Holliday junctions by eukaryotic DNA topoisomerase I.
1996,
Pubmed
Sobeck,
Fanconi anemia proteins are required to prevent accumulation of replication-associated DNA double-strand breaks.
2006,
Pubmed
,
Xenbase
Taniguchi,
S-phase-specific interaction of the Fanconi anemia protein, FANCD2, with BRCA1 and RAD51.
2002,
Pubmed
Thomashevski,
The Fanconi anemia core complex forms four complexes of different sizes in different subcellular compartments.
2004,
Pubmed
Wang,
Functional interaction of monoubiquitinated FANCD2 and BRCA2/FANCD1 in chromatin.
2004,
Pubmed
Xia,
Fanconi anemia is associated with a defect in the BRCA2 partner PALB2.
2007,
Pubmed
Xirodimas,
Different effects of p14ARF on the levels of ubiquitinated p53 and Mdm2 in vivo.
2001,
Pubmed
Zembutsu,
De novo assembly of genuine replication forks on an immobilized circular plasmid in Xenopus egg extracts.
2006,
Pubmed
,
Xenbase
Zhu,
An ATR- and BRCA1-mediated Fanconi anemia pathway is required for activating the G2/M checkpoint and DNA damage repair upon rereplication.
2006,
Pubmed
Zhu,
Activation of fanconi anemia pathway in cells with re-replicated DNA.
2006,
Pubmed
