Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Biol
1989 Feb 01;92:515-22. doi: 10.1128/mcb.9.2.515-522.1989.
Show Gene links
Show Anatomy links
The sarcomeric actin CArG-binding factor is indistinguishable from the c-fos serum response factor.
Boxer LM
,
Prywes R
,
Roeder RG
,
Kedes L
.
???displayArticle.abstract???
The c-fos serum response element (SRE) and a sarcomeric actin promoter element (CArG box) are similar in sequence and are recognized, respectively, by the serum response factor (SRF) and the CArG-binding factor (CBF). Although the transcriptional controls for the c-fos and sarcomeric actin genes are rather different, SRF and CBF have been found to be indistinguishable by all criteria tested. They exhibited similar chromatographic properties, sedimentation rates, and temperature stabilities. In mobility shift assays, the SRE competed more strongly than the actin CArG box for formation of either the SRF-SRE or the CBF-CArG complex. The symmetric inverted repeat of the left side of the Xenopus cytoskeletal actin SRE also competed, even more strongly, for each complex. The site-specific binding of each protein was inhibited both by orthophenanthroline, whose effects were reversed by zinc addition, and by treatment with potato acid phosphatase. Furthermore, immune serum raised against the c-fosSRF also recognized the actin CBF. We discuss how transcriptional control of these diverse genes might be obtained with a single similar factor.
Berg,
Proposed structure for the zinc-binding domains from transcription factor IIIA and related proteins.
1988, Pubmed
Berg,
Proposed structure for the zinc-binding domains from transcription factor IIIA and related proteins.
1988,
Pubmed
Berg,
Potential metal-binding domains in nucleic acid binding proteins.
1986,
Pubmed
,
Xenbase
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Bravo,
Stimulation and inhibition of growth by EGF in different A431 cell clones is accompanied by the rapid induction of c-fos and c-myc proto-oncogenes.
1985,
Pubmed
Chodosh,
A single polypeptide possesses the binding and transcription activities of the adenovirus major late transcription factor.
1986,
Pubmed
Chodosh,
Human CCAAT-binding proteins have heterologous subunits.
1988,
Pubmed
Dignam,
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
1983,
Pubmed
Dorn,
A multiplicity of CCAAT box-binding proteins.
1987,
Pubmed
Dynan,
Control of eukaryotic messenger RNA synthesis by sequence-specific DNA-binding proteins.
,
Pubmed
Fletcher,
Purification and characterization of OTF-1, a transcription factor regulating cell cycle expression of a human histone H2b gene.
1987,
Pubmed
Frankel,
Tat protein from human immunodeficiency virus forms a metal-linked dimer.
1988,
Pubmed
Fried,
Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.
1981,
Pubmed
Gilman,
Multiple protein-binding sites in the 5'-flanking region regulate c-fos expression.
1986,
Pubmed
Greenberg,
Nerve growth factor and epidermal growth factor induce rapid transient changes in proto-oncogene transcription in PC12 cells.
1985,
Pubmed
Greenberg,
Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogene.
,
Pubmed
Gunning,
alpha-skeletal and alpha-cardiac actin genes are coexpressed in adult human skeletal muscle and heart.
1983,
Pubmed
Gustafson,
Interaction of nuclear proteins with muscle-specific regulatory sequences of the human cardiac alpha-actin promoter.
1988,
Pubmed
Kadonaga,
Affinity purification of sequence-specific DNA binding proteins.
1986,
Pubmed
Kruijer,
Induction of the proto-oncogene fos by nerve growth factor.
1985,
Pubmed
Kruijer,
Platelet-derived growth factor induces rapid but transient expression of the c-fos gene and protein.
,
Pubmed
Maniatis,
Regulation of inducible and tissue-specific gene expression.
1987,
Pubmed
Mayer,
Expression of the genes coding for the skeletal muscle and cardiac actions in the heart.
1984,
Pubmed
Miller,
Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes.
1985,
Pubmed
,
Xenbase
Minty,
Upstream regions of the human cardiac actin gene that modulate its transcription in muscle cells: presence of an evolutionarily conserved repeated motif.
1986,
Pubmed
Minty,
Two-level regulation of cardiac actin gene transcription: muscle-specific modulating factors can accumulate before gene activation.
1986,
Pubmed
Miwa,
Duplicated CArG box domains have positive and mutually dependent regulatory roles in expression of the human alpha-cardiac actin gene.
1987,
Pubmed
Miwa,
CArG boxes in the human cardiac alpha-actin gene are core binding sites for positive trans-acting regulatory factors.
1987,
Pubmed
Moore,
c-fos and c-myc gene activation, ionic signals, and DNA synthesis in thymocytes.
1986,
Pubmed
Müller,
Induction of c-fos gene and protein by growth factors precedes activation of c-myc.
,
Pubmed
Muscat,
A common factor regulates skeletal and cardiac alpha-actin gene transcription in muscle.
1988,
Pubmed
Muscat,
Multiple 5'-flanking regions of the human alpha-skeletal actin gene synergistically modulate muscle-specific expression.
1987,
Pubmed
Peterson,
Complex protein binding within the mouse immunoglobulin heavy-chain enhancer.
1987,
Pubmed
Posorske,
Methionyl-tRNA synthetase of Escherichia coli. A zinc metalloprotein.
1979,
Pubmed
Prywes,
Phosphorylation of serum response factor, a factor that binds to the serum response element of the c-FOS enhancer.
1988,
Pubmed
Prywes,
Purification of the c-fos enhancer-binding protein.
1987,
Pubmed
Rosenfeld,
Purification of nuclear factor I by DNA recognition site affinity chromatography.
1986,
Pubmed
Scheidereit,
Identification and purification of a human lymphoid-specific octamer-binding protein (OTF-2) that activates transcription of an immunoglobulin promoter in vitro.
1987,
Pubmed
Schröter,
Purification of intercalator-released p67, a polypeptide that interacts specifically with the c-fos serum response element.
1987,
Pubmed
Singh,
A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes.
,
Pubmed
Taylor,
Nucleotide sequence and expression of the human skeletal alpha-actin gene: evolution of functional regulatory domains.
1988,
Pubmed
Treisman,
Identification and purification of a polypeptide that binds to the c-fos serum response element.
1987,
Pubmed
Treisman,
Identification of a protein-binding site that mediates transcriptional response of the c-fos gene to serum factors.
1986,
Pubmed
Vandekerckhove,
Simultaneous expression of skeletal muscle and heart actin proteins in various striated muscle tissues and cells. A quantitative determination of the two actin isoforms.
1986,
Pubmed
Westin,
Heavy metal ions in transcription factors from HeLa cells: Sp1, but not octamer transcription factor requires zinc for DNA binding and for activator function.
1988,
Pubmed
Yaffe,
Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle.
,
Pubmed
