XB-ART-50376
Dev Biol
2015 Dec 15;4082:180-7. doi: 10.1016/j.ydbio.2015.02.003.
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An oncologist׳s friend: How Xenopus contributes to cancer research.
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One of the most striking features of the Xenopus system is the versatility in providing a unique range of both in vitro and in vivo models that are rapid, accessible and easily manipulated. Here we present an overview of the diverse contribution that Xenopus has made to advance our understanding of tumour biology and behaviour; a contribution that goes beyond the traditional view of Xenopus as a developmental model organism. From the utility of the egg and oocyte extract system to the use of whole embryos as developmental or induced tumour models, the Xenopus system has been fundamental to investigation of cell cycle mechanisms, cell metabolism, cell signalling and cell behaviour, and has allowed an increasing appreciation of the parallels between early development and the pathogenesis of tumour progression and metastasis. Although not the prototypical oncological model system, we propose that Xenopus is an adaptable and multifunctional tool in the oncologist׳s arsenal.
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G0500101 Medical Research Council , G0700758 Medical Research Council , MRC_G0500101 Medical Research Council , MRC_G0700758 Medical Research Council
Species referenced: Xenopus
Genes referenced: ctnnb1 tubb2b
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Phenotypes: Xla Wt + ctnnb1 (fig.1.b)
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Fig. 1. (A) A secondary axis can be induced in developing Xenopus embryos by injection of RNA encoding β-catenin into a ventral cell of 4-cell stage embryos. Ventral cells are usually distinguished by their larger size and darker pigment compared to dorsal cells. For detailed methods see ( Kuhl and Pandur, 2008a). (B) The duplicated axis is visible in neurula stage embryos within 2 days of injection. Embryos in these images have undergone in situ hybridisation for neural-β-tubulin to illustrate the bilateral stripes of primary neurons and trigeminal ganglia. Embryos can be exposed to a range of compounds during development to assay for ability of the compound to inhibit axis duplication. Alternatively, RNA encoding proteins of interest can be injected into the ventral cells to assay for ability of the protein to induce a secondary axis. |
References [+] :
Acloque,
The physiology and pathology of the EMT. Meeting on the epithelial-mesenchymal transition.
2008, Pubmed
Acloque, The physiology and pathology of the EMT. Meeting on the epithelial-mesenchymal transition. 2008, Pubmed
Acloque, Epithelial-mesenchymal transitions: the importance of changing cell state in development and disease. 2009, Pubmed
Ali, Cell cycle-regulated multi-site phosphorylation of Neurogenin 2 coordinates cell cycling with differentiation during neurogenesis. 2011, Pubmed , Xenbase
Ali, The phosphorylation status of Ascl1 is a key determinant of neuronal differentiation and maturation in vivo and in vitro. 2014, Pubmed , Xenbase
Allegrucci, Epigenetic reprogramming of breast cancer cells with oocyte extracts. 2011, Pubmed , Xenbase
Anderson, Transport of the photodynamic therapy agent 5-aminolevulinic acid by distinct H+-coupled nutrient carriers coexpressed in the small intestine. 2010, Pubmed , Xenbase
Asfari, Mycobacterium-induced infectious granuloma in Xenopus: histopathology and transmissibility. 1988, Pubmed , Xenbase
Asfari, Transplantation studies of a putative lymphosarcoma of Xenopus. 1988, Pubmed , Xenbase
Bailey, Cancer metastasis facilitated by developmental pathways: Sonic hedgehog, Notch, and bone morphogenic proteins. 2007, Pubmed
Balls, Lymphosarcoma in the South African clawed toad, Xenopus laevis: a virus tumor. 1965, Pubmed , Xenbase
Berry, The ALK(F1174L) mutation potentiates the oncogenic activity of MYCN in neuroblastoma. 2012, Pubmed
Berthet, Cdk2 knockout mice are viable. 2003, Pubmed
Bian, Epigenetic marks in somatic chromatin are remodelled to resemble pluripotent nuclei by amphibian oocyte extracts. 2009, Pubmed
Blow, Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs. 1986, Pubmed , Xenbase
Blow, Control of chromosomal DNA replication in the early Xenopus embryo. 2001, Pubmed , Xenbase
Browaeys-Poly, Akt interaction with PLC(gamma) regulates the G(2)/M transition triggered by FGF receptors from MDA-MB-231 breast cancer cells. 2009, Pubmed , Xenbase
Bui, Cancer immunosurveillance, immunoediting and inflammation: independent or interdependent processes? 2007, Pubmed
Cailliau, FGF receptor phosphotyrosine 766 is a target for Grb14 to inhibit MDA-MB-231 human breast cancer cell signaling. 2005, Pubmed , Xenbase
Carruthers, Depletion of the cell-cycle inhibitor p27(Xic1) impairs neuronal differentiation and increases the number of ElrC(+) progenitor cells in Xenopus tropicalis. 2003, Pubmed , Xenbase
Cekanova, Animal models and therapeutic molecular targets of cancer: utility and limitations. 2014, Pubmed
Cha, Using oocytes for Wnt signaling assays: paracrine assays and Wnt-conditioned medium. 2010, Pubmed , Xenbase
Cheong, Spontaneous thyroid-containing teratoma associated with impaired development in the African clawed frog, Xenopus laevis. 2000, Pubmed , Xenbase
Chernet, Transmembrane voltage potential of somatic cells controls oncogene-mediated tumorigenesis at long-range. 2014, Pubmed , Xenbase
Cross, Learning about cancer from frogs: analysis of mitotic spindles in Xenopus egg extracts. 2009, Pubmed , Xenbase
Dahmane, Activation of the transcription factor Gli1 and the Sonic hedgehog signalling pathway in skin tumours. 1997, Pubmed , Xenbase
Deming, Study of apoptosis in vitro using the Xenopus egg extract reconstitution system. 2006, Pubmed , Xenbase
De Robertis, Identification and characterization of a small-molecule inhibitor of Wnt signaling in glioblastoma cells. 2013, Pubmed , Xenbase
Desai, The use of Xenopus egg extracts to study mitotic spindle assembly and function in vitro. 1999, Pubmed , Xenbase
Dikovskaya, The adenomatous polyposis coli protein contributes to normal compaction of mitotic chromatin. 2012, Pubmed , Xenbase
Du Pasquier, In vitro growth of thymic tumor cell lines from Xenopus. 1992, Pubmed , Xenbase
Dworkin, Metabolic regulation during early frog development: glycogenic flux in Xenopus oocytes, eggs, and embryos. 1989, Pubmed , Xenbase
Earley, Tissue culture of a mixed cell thymic tumor from Xenopus laevis. 1995, Pubmed , Xenbase
Felix, A post-ribosomal supernatant from activated Xenopus eggs that displays post-translationally regulated oscillation of its cdc2+ mitotic kinase activity. 1989, Pubmed , Xenbase
Félix, Triggering of cyclin degradation in interphase extracts of amphibian eggs by cdc2 kinase. 1990, Pubmed , Xenbase
Glackin, Targeting the Twist and Wnt signaling pathways in metastatic breast cancer. 2014, Pubmed
Goding, Cancer: pathological nuclear reprogramming? 2014, Pubmed
Goyos, Tumorigenesis and anti-tumor immune responses in Xenopus. 2009, Pubmed , Xenbase
Gradl, Keeping a close eye on Wnt-1/wg signaling in Xenopus. 1999, Pubmed , Xenbase
GURDON, Sexually mature individuals of Xenopus laevis from the transplantation of single somatic nuclei. 1958, Pubmed , Xenbase
Halley-Stott, Nuclear reprogramming. 2013, Pubmed
Halley-Stott, Epigenetic memory in the context of nuclear reprogramming and cancer. 2013, Pubmed
Hanahan, Hallmarks of cancer: the next generation. 2011, Pubmed
Hanahan, The hallmarks of cancer. 2000, Pubmed
Hardwick, Nervous decision-making: to divide or differentiate. 2014, Pubmed
Harper, The mammalian cell cycle: an overview. 2005, Pubmed
Hashimoto, Studying DNA replication fork stability in Xenopus egg extract. 2011, Pubmed , Xenbase
Haynes-Gilmore, A critical role of non-classical MHC in tumor immune evasion in the amphibian Xenopus model. 2014, Pubmed , Xenbase
Hikasa, Wnt signaling in vertebrate axis specification. 2013, Pubmed , Xenbase
Hindley, Post-translational modification of Ngn2 differentially affects transcription of distinct targets to regulate the balance between progenitor maintenance and differentiation. 2012, Pubmed , Xenbase
Hingorani, Trp53R172H and KrasG12D cooperate to promote chromosomal instability and widely metastatic pancreatic ductal adenocarcinoma in mice. 2005, Pubmed
Ishimura, Oncogenic Met receptor induces ectopic structures in Xenopus embryos. 2006, Pubmed , Xenbase
Joukov, The BRCA1/BARD1 heterodimer modulates ran-dependent mitotic spindle assembly. 2006, Pubmed , Xenbase
Kalluri, The basics of epithelial-mesenchymal transition. 2009, Pubmed
Kühl, Measuring CamKII activity in Xenopus embryos as a read-out for non-canonical Wnt signaling. 2008, Pubmed , Xenbase
Kühl, Non-canonical Wnt signaling in Xenopus: regulation of axis formation and gastrulation. 2002, Pubmed , Xenbase
Kühl, Dorsal axis duplication as a functional readout for Wnt activity. 2008, Pubmed , Xenbase
Kvist, The use of Xenopus oocytes in drug screening. 2011, Pubmed , Xenbase
Laiho, Cell cycle control, DNA damage checkpoints and cancer. 2003, Pubmed
Landais, A novel cell-free screen identifies a potent inhibitor of the Fanconi anemia pathway. 2009, Pubmed , Xenbase
Lander, Interactions between Twist and other core epithelial-mesenchymal transition factors are controlled by GSK3-mediated phosphorylation. 2013, Pubmed , Xenbase
Laskey, Chromatin assembly and transcription in eggs and oocytes of Xenopus laevis. 1978, Pubmed , Xenbase
Leno, The nuclear membrane prevents replication of human G2 nuclei but not G1 nuclei in Xenopus egg extract. 1992, Pubmed , Xenbase
Leno, DNA replication in cell-free extracts from Xenopus laevis. 1991, Pubmed , Xenbase
Liu, Cloning and characterization of glioma BK, a novel BK channel isoform highly expressed in human glioma cells. 2002, Pubmed , Xenbase
Liu, Human homologue of cement gland protein, a novel metastasis inducer associated with breast carcinomas. 2005, Pubmed , Xenbase
Lujan, The many roads to Rome: induction of neural precursor cells from fibroblasts. 2012, Pubmed
Ma, The relationship between early embryo development and tumourigenesis. 2010, Pubmed
Mani, Mesenchyme Forkhead 1 (FOXC2) plays a key role in metastasis and is associated with aggressive basal-like breast cancers. 2007, Pubmed
McDowell, Complex domain interactions regulate stability and activity of closely related proneural transcription factors. 2014, Pubmed , Xenbase
Mimori-Kiyosue, Adenomatous polyposis coli (APC) protein regulates epithelial cell migration and morphogenesis via PDZ domain-based interactions with plasma membranes. 2007, Pubmed , Xenbase
Minshull, The A- and B-type cyclin associated cdc2 kinases in Xenopus turn on and off at different times in the cell cycle. 1990, Pubmed , Xenbase
Miyata, Selective inhibition of the growth of cancer cells by diterpenes selected with embryonic cells of Xenopus. 2004, Pubmed , Xenbase
Nakaya, EMT in developmental morphogenesis. 2013, Pubmed
Newport, A major developmental transition in early Xenopus embryos: II. Control of the onset of transcription. 1982, Pubmed , Xenbase
Newport, A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage. 1982, Pubmed , Xenbase
Nutt, The Xenopus oocyte: a model for studying the metabolic regulation of cancer cell death. 2012, Pubmed , Xenbase
Ny, A transgenic Xenopus laevis reporter model to study lymphangiogenesis. 2013, Pubmed , Xenbase
Ny, A genetic Xenopus laevis tadpole model to study lymphangiogenesis. 2005, Pubmed , Xenbase
Okudaira, Kinetic analyses of trans-1-amino-3-[18F]fluorocyclobutanecarboxylic acid transport in Xenopus laevis oocytes expressing human ASCT2 and SNAT2. 2013, Pubmed , Xenbase
Ortega, Cyclin-dependent kinase 2 is essential for meiosis but not for mitotic cell division in mice. 2003, Pubmed
Pakladok, Up-regulation of hERG K⁺ channels by B-RAF. 2014, Pubmed , Xenbase
Pakladok, Stimulation of the Na(+)-coupled glucose transporter SGLT1 by B-RAF. 2012, Pubmed , Xenbase
Pennisi, How a growth control path takes a wrong turn to cancer. 1998, Pubmed
Philpott, The Xenopus cell cycle: an overview. 2008, Pubmed , Xenbase
Rane, Loss of Cdk4 expression causes insulin-deficient diabetes and Cdk4 activation results in beta-islet cell hyperplasia. 1999, Pubmed
Richard-Parpaillon, G1/S phase cyclin-dependent kinase overexpression perturbs early development and delays tissue-specific differentiation in Xenopus. 2004, Pubmed , Xenbase
Robert, Comparative study of tumorigenesis and tumor immunity in invertebrates and nonmammalian vertebrates. 2010, Pubmed
Robert, Evolution of immune surveillance and tumor immunity: studies in Xenopus. 1998, Pubmed , Xenbase
Robert, Effects of thymectomy and tolerance induction on tumor immunity in adult Xenopus laevis. 1997, Pubmed , Xenbase
Robert, Lymphoid tumors of Xenopus laevis with different capacities for growth in larvae and adults. 1994, Pubmed , Xenbase
Rosania, A cyclin-dependent kinase inhibitor inducing cancer cell differentiation: biochemical identification using Xenopus egg extracts. 1999, Pubmed , Xenbase
Ruben, Cancer resistance in amphibians. 2007, Pubmed , Xenbase
Sadikovic, Cause and consequences of genetic and epigenetic alterations in human cancer. 2008, Pubmed
Saka, Spatial and temporal patterns of cell division during early Xenopus embryogenesis. 2001, Pubmed , Xenbase
Santamaria, Cyclins and CDKS in development and cancer: lessons from genetically modified mice. 2006, Pubmed
Schmitt, Engineering Xenopus embryos for phenotypic drug discovery screening. 2014, Pubmed , Xenbase
Srinivasan, Study of cell cycle checkpoints using Xenopus cell-free extracts. 2011, Pubmed , Xenbase
Taube, Core epithelial-to-mesenchymal transition interactome gene-expression signature is associated with claudin-low and metaplastic breast cancer subtypes. 2010, Pubmed
Theveneau, Neural crest delamination and migration: from epithelium-to-mesenchyme transition to collective cell migration. 2012, Pubmed , Xenbase
Thiery, Epithelial-mesenchymal transitions in development and disease. 2009, Pubmed
Tsutsui, Targeted disruption of CDK4 delays cell cycle entry with enhanced p27(Kip1) activity. 1999, Pubmed
Tumova, Monensin inhibits canonical Wnt signaling in human colorectal cancer cells and suppresses tumor growth in multiple intestinal neoplasia mice. 2014, Pubmed , Xenbase
Ureta, Frog oocytes: a living test tube for studies on metabolic regulation. 2001, Pubmed , Xenbase
Vernon, A single cdk inhibitor, p27Xic1, functions beyond cell cycle regulation to promote muscle differentiation in Xenopus. 2003, Pubmed , Xenbase
Vernon, The cdk inhibitor p27Xic1 is required for differentiation of primary neurones in Xenopus. 2003, Pubmed , Xenbase
Vernon, Tumor metastasis: a new twist on epithelial-mesenchymal transitions. 2004, Pubmed , Xenbase
Vernon, Notch targets the Cdk inhibitor Xic1 to regulate differentiation but not the cell cycle in neurons. 2006, Pubmed , Xenbase
Vosper, Ubiquitylation on canonical and non-canonical sites targets the transcription factor neurogenin for ubiquitin-mediated proteolysis. 2009, Pubmed , Xenbase
Waaler, A novel tankyrase inhibitor decreases canonical Wnt signaling in colon carcinoma cells and reduces tumor growth in conditional APC mutant mice. 2012, Pubmed , Xenbase
Waaler, Novel synthetic antagonists of canonical Wnt signaling inhibit colorectal cancer cell growth. 2011, Pubmed , Xenbase
Wallingford, Tumors in tadpoles: the Xenopus embryo as a model system for the study of tumorigenesis. 1999, Pubmed , Xenbase
Wallingford, p53 activity is essential for normal development in Xenopus. 1997, Pubmed , Xenbase
Wang, Remodeling epigenetic modifications at tumor suppressor gene promoters with bovine oocyte extract. 2013, Pubmed
Wang, Specific reversal of tumor-suppressor gene promoter hypermethylation with bovine oocyte extract. 2013, Pubmed
Wang, Wnt/Planar cell polarity signaling: a new paradigm for cancer therapy. 2009, Pubmed
Wei, ZC88, a novel 4-amino piperidine analog, inhibits the growth of neuroblastoma cells through blocking hERG potassium channel. 2013, Pubmed , Xenbase
Whitman, Involvement of p21ras in Xenopus mesoderm induction. 1992, Pubmed , Xenbase
Willis, Study of the DNA damage checkpoint using Xenopus egg extracts. 2012, Pubmed , Xenbase
Woodland, Some studies on early embryonic development relevant to the study of cancer. 1974, Pubmed , Xenbase
Wu, VentX trans-activates p53 and p16ink4a to regulate cellular senescence. 2011, Pubmed , Xenbase
Wu, Notch signaling and its role in breast cancer. 2007, Pubmed
Xi, Wnt signaling pathway: implications for therapy in lung cancer and bone metastasis. 2014, Pubmed
Xie, Developmental biology informs cancer: the emerging role of the hedgehog signaling pathway in upper gastrointestinal cancers. 2003, Pubmed
Yang, Twist, a master regulator of morphogenesis, plays an essential role in tumor metastasis. 2004, Pubmed
Yang, Overexpression of a novel Xenopus rel mRNA gene induces tumors in early embryos. 1998, Pubmed , Xenbase
Yew, Proteolysis and DNA replication: the CDC34 requirement in the Xenopus egg cell cycle. 1997, Pubmed , Xenbase
You, Rapid activation of ATM on DNA flanking double-strand breaks. 2007, Pubmed , Xenbase
Zyłkiewicz, Xenopus egg extracts as a simplified model system for structure-function studies of dynein regulators. 2014, Pubmed , Xenbase