Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
The fundamental process of nucleocytoplasmic transport takes place through the nuclear pore. Peripheral pore structures are presumably poised to interact with transport receptors and their cargo as these receptor complexes first encounter the pore. One such peripheral structure likely to play an important role in nuclear export is the basket structure located on the nuclear side of the pore. At present, Nup153 is the only nucleoporin known to localize to the surface of this basket, suggesting that Nup153 is potentially one of the first pore components an RNA or protein encounters during export. In this study, anti-Nup153 antibodies were used to probe the role of Nup153 in nuclear export in Xenopus oocytes. We found that Nup153 antibodies block three major classes of RNA export, that of snRNA, mRNA, and 5S rRNA. Nup153 antibodies also block the NES protein export pathway, specifically the export of the HIV Rev protein, as well as Rev-dependent RNA export. Not all export was blocked; Nup153 antibodies did not impede the export of tRNA or the recycling of importin beta to the cytoplasm. The specific antibodies used here also did not affect nuclear import, whether mediated by importin alpha/beta or by transportin. Overall, the results indicate that Nup153 is crucial to multiple classes of RNA and protein export, being involved at a vital juncture point in their export pathways. This juncture point appears to be one that is bypassed by tRNA during its export. We asked whether a physical interaction between RNA and Nup153 could be observed, using homoribopolymers as sequence-independent probes for interaction. Nup153, unlike four other nucleoporins including Nup98, associated strongly with poly(G) and significantly with poly(U). Thus, Nup153 is unique among the nucleoporins tested in its ability to interact with RNA and must do so either directly or indirectly through an adaptor protein. These results suggest a unique mechanistic role for Nup153 in the export of multiple cargos.
Adam,
Cytosolic proteins that specifically bind nuclear location signals are receptors for nuclear import.
1991, Pubmed
Adam,
Cytosolic proteins that specifically bind nuclear location signals are receptors for nuclear import.
1991,
Pubmed
Aitchison,
Kap104p: a karyopherin involved in the nuclear transport of messenger RNA binding proteins.
1996,
Pubmed
Arts,
Identification of a nuclear export receptor for tRNA.
1998,
Pubmed
,
Xenbase
Bailer,
Nup116p and nup100p are interchangeable through a conserved motif which constitutes a docking site for the mRNA transport factor gle2p.
1998,
Pubmed
,
Xenbase
Bastos,
Targeting and function in mRNA export of nuclear pore complex protein Nup153.
1996,
Pubmed
Bastos,
Nup84, a novel nucleoporin that is associated with CAN/Nup214 on the cytoplasmic face of the nuclear pore complex.
1997,
Pubmed
Bataillé,
Cytoplasmic transport of ribosomal subunits microinjected into the Xenopus laevis oocyte nucleus: a generalized, facilitated process.
1990,
Pubmed
,
Xenbase
Belanger,
Genetic and physical interactions between Srp1p and nuclear pore complex proteins Nup1p and Nup2p.
1994,
Pubmed
Bonifaci,
Karyopherin beta2 mediates nuclear import of a mRNA binding protein.
1997,
Pubmed
Chi,
Sequence and characterization of cytoplasmic nuclear protein import factor p97.
1995,
Pubmed
Clarkson,
Nuclear protein import is decreased by engineered mutants of nuclear transport factor 2 (NTF2) that do not bind GDP-Ran.
1997,
Pubmed
Cole,
Nucleocytoplasmic transport: driving and directing transport.
1998,
Pubmed
Corbett,
Nucleocytoplasmic transport of macromolecules.
1997,
Pubmed
Corbett,
The NTF2 gene encodes an essential, highly conserved protein that functions in nuclear transport in vivo.
1996,
Pubmed
Cordes,
Identification of protein p270/Tpr as a constitutive component of the nuclear pore complex-attached intranuclear filaments.
1997,
Pubmed
,
Xenbase
Cordes,
Intranuclear filaments containing a nuclear pore complex protein.
1993,
Pubmed
,
Xenbase
Dabauvalle,
Monoclonal antibodies to a Mr 68,000 pore complex glycoprotein interfere with nuclear protein uptake in Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Fabre,
Nup145p is required for nuclear export of mRNA and binds homopolymeric RNA in vitro via a novel conserved motif.
1994,
Pubmed
Featherstone,
A monoclonal antibody against the nuclear pore complex inhibits nucleocytoplasmic transport of protein and RNA in vivo.
1988,
Pubmed
,
Xenbase
Feldherr,
The nuclear import factor p10 regulates the functional size of the nuclear pore complex during oogenesis.
1998,
Pubmed
,
Xenbase
Ferrigno,
Regulated nucleo/cytoplasmic exchange of HOG1 MAPK requires the importin beta homologs NMD5 and XPO1.
1998,
Pubmed
Finlay,
Inhibition of in vitro nuclear transport by a lectin that binds to nuclear pores.
1987,
Pubmed
,
Xenbase
Fischer,
Evidence that HIV-1 Rev directly promotes the nuclear export of unspliced RNA.
1994,
Pubmed
,
Xenbase
Fischer,
The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs.
1995,
Pubmed
Fornerod,
The human homologue of yeast CRM1 is in a dynamic subcomplex with CAN/Nup214 and a novel nuclear pore component Nup88.
1997,
Pubmed
Fornerod,
CRM1 is an export receptor for leucine-rich nuclear export signals.
1997,
Pubmed
,
Xenbase
Fukuda,
CRM1 is responsible for intracellular transport mediated by the nuclear export signal.
1997,
Pubmed
,
Xenbase
Goldberg,
Structural and functional organization of the nuclear envelope.
1995,
Pubmed
,
Xenbase
Görlich,
A novel class of RanGTP binding proteins.
1997,
Pubmed
,
Xenbase
Görlich,
Two different subunits of importin cooperate to recognize nuclear localization signals and bind them to the nuclear envelope.
1995,
Pubmed
,
Xenbase
Görlich,
Isolation of a protein that is essential for the first step of nuclear protein import.
1994,
Pubmed
,
Xenbase
Grandi,
Nup93, a vertebrate homologue of yeast Nic96p, forms a complex with a novel 205-kDa protein and is required for correct nuclear pore assembly.
1997,
Pubmed
,
Xenbase
Grote,
Mapping of nucleoporins to the center of the nuclear pore complex by post-embedding immunogold electron microscopy.
1995,
Pubmed
Guan,
Structural analysis of the p62 complex, an assembly of O-linked glycoproteins that localizes near the central gated channel of the nuclear pore complex.
1995,
Pubmed
Hamm,
An abundant U6 snRNP found in germ cells and embryos of Xenopus laevis.
1989,
Pubmed
,
Xenbase
Hellmuth,
Yeast Los1p has properties of an exportin-like nucleocytoplasmic transport factor for tRNA.
1998,
Pubmed
Hope,
Viral RNA export.
1997,
Pubmed
Imamoto,
In vivo evidence for involvement of a 58 kDa component of nuclear pore-targeting complex in nuclear protein import.
1995,
Pubmed
,
Xenbase
Iovine,
The GLFG repetitive region of the nucleoporin Nup116p interacts with Kap95p, an essential yeast nuclear import factor.
1995,
Pubmed
Izaurralde,
Transport of macromolecules between the nucleus and the cytoplasm.
1998,
Pubmed
Jäkel,
Importin beta, transportin, RanBP5 and RanBP7 mediate nuclear import of ribosomal proteins in mammalian cells.
1998,
Pubmed
Jarmolowski,
Nuclear export of different classes of RNA is mediated by specific factors.
1994,
Pubmed
,
Xenbase
Kellogg,
Members of the NAP/SET family of proteins interact specifically with B-type cyclins.
1995,
Pubmed
,
Xenbase
Kraemer,
The human CAN protein, a putative oncogene product associated with myeloid leukemogenesis, is a nuclear pore complex protein that faces the cytoplasm.
1994,
Pubmed
Kutay,
Identification of a tRNA-specific nuclear export receptor.
1998,
Pubmed
,
Xenbase
Kutay,
Dominant-negative mutants of importin-beta block multiple pathways of import and export through the nuclear pore complex.
1997,
Pubmed
,
Xenbase
Lund,
Nonaqueous isolation of transcriptionally active nuclei from Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Malim,
Functional dissection of the HIV-1 Rev trans-activator--derivation of a trans-dominant repressor of Rev function.
1989,
Pubmed
Matunis,
Characterization and primary structure of the poly(C)-binding heterogeneous nuclear ribonucleoprotein complex K protein.
1992,
Pubmed
Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed
Meyer,
Nuclear transport of human immunodeficiency virus type 1, visna virus, and equine infectious anemia virus Rev proteins: identification of a family of transferable nuclear export signals.
1996,
Pubmed
Michael,
A nuclear export signal in hnRNP A1: a signal-mediated, temperature-dependent nuclear protein export pathway.
1995,
Pubmed
Moore,
Purification of a Ran-interacting protein that is required for protein import into the nucleus.
1994,
Pubmed
,
Xenbase
Moroianu,
Previously identified protein of uncertain function is karyopherin alpha and together with karyopherin beta docks import substrate at nuclear pore complexes.
1995,
Pubmed
Neuman de Vegvar,
Nucleocytoplasmic transport and processing of small nuclear RNA precursors.
1990,
Pubmed
,
Xenbase
Neville,
The importin-beta family member Crm1p bridges the interaction between Rev and the nuclear pore complex during nuclear export.
1997,
Pubmed
Ohno,
Nucleocytoplasmic transport: the last 200 nanometers.
1998,
Pubmed
Ossareh-Nazari,
Evidence for a role of CRM1 in signal-mediated nuclear protein export.
1997,
Pubmed
Panté,
Interactions and three-dimensional localization of a group of nuclear pore complex proteins.
1994,
Pubmed
,
Xenbase
Paschal,
Identification of NTF2, a cytosolic factor for nuclear import that interacts with nuclear pore complex protein p62.
1995,
Pubmed
Pasquinelli,
The constitutive transport element (CTE) of Mason-Pfizer monkey virus (MPMV) accesses a cellular mRNA export pathway.
1997,
Pubmed
,
Xenbase
Pasquinelli,
Inhibition of mRNA export in vertebrate cells by nuclear export signal conjugates.
1997,
Pubmed
,
Xenbase
Pemberton,
A distinct and parallel pathway for the nuclear import of an mRNA-binding protein.
1997,
Pubmed
Percipalle,
Molecular interactions between the importin alpha/beta heterodimer and proteins involved in vertebrate nuclear protein import.
1997,
Pubmed
Pollard,
A novel receptor-mediated nuclear protein import pathway.
1996,
Pubmed
Powers,
Reconstituted nuclei depleted of a vertebrate GLFG nuclear pore protein, p97, import but are defective in nuclear growth and replication.
1995,
Pubmed
,
Xenbase
Powers,
The vertebrate GLFG nucleoporin, Nup98, is an essential component of multiple RNA export pathways.
1997,
Pubmed
,
Xenbase
Radu,
Identification of a protein complex that is required for nuclear protein import and mediates docking of import substrate to distinct nucleoporins.
1995,
Pubmed
,
Xenbase
Radu,
The peptide repeat domain of nucleoporin Nup98 functions as a docking site in transport across the nuclear pore complex.
1995,
Pubmed
Rexach,
Protein import into nuclei: association and dissociation reactions involving transport substrate, transport factors, and nucleoporins.
1995,
Pubmed
Rosenblum,
A nuclear import pathway for a protein involved in tRNA maturation.
1997,
Pubmed
Rout,
A distinct nuclear import pathway used by ribosomal proteins.
1997,
Pubmed
Saavedra,
The simian retrovirus-1 constitutive transport element, unlike the HIV-1 RRE, uses factors required for cellular mRNA export.
1997,
Pubmed
,
Xenbase
Senger,
Mtr10p functions as a nuclear import receptor for the mRNA-binding protein Npl3p.
1998,
Pubmed
Shah,
Separate nuclear import pathways converge on the nucleoporin Nup153 and can be dissected with dominant-negative inhibitors.
,
Pubmed
,
Xenbase
Shah,
Major binding sites for the nuclear import receptor are the internal nucleoporin Nup153 and the adjacent nuclear filament protein Tpr.
1998,
Pubmed
,
Xenbase
Simos,
Nuclear pore proteins are involved in the biogenesis of functional tRNA.
1996,
Pubmed
Smythe,
Systems for the study of nuclear assembly, DNA replication, and nuclear breakdown in Xenopus laevis egg extracts.
1991,
Pubmed
,
Xenbase
Stade,
Exportin 1 (Crm1p) is an essential nuclear export factor.
1997,
Pubmed
Sukegawa,
A nuclear pore complex protein that contains zinc finger motifs, binds DNA, and faces the nucleoplasm.
1993,
Pubmed
Terns,
Multiple cis-acting signals for export of pre-U1 snRNA from the nucleus.
1993,
Pubmed
,
Xenbase
Terns,
3'-end-dependent formation of U6 small nuclear ribonucleoprotein particles in Xenopus laevis oocyte nuclei.
1992,
Pubmed
,
Xenbase
Terns,
Retention and 5' cap trimethylation of U3 snRNA in the nucleus.
1994,
Pubmed
,
Xenbase
Turner,
Expression of achaete-scute homolog 3 in Xenopus embryos converts ectodermal cells to a neural fate.
1994,
Pubmed
,
Xenbase
Ullman,
Nuclear export receptors: from importin to exportin.
1997,
Pubmed
Weis,
Identification of hSRP1 alpha as a functional receptor for nuclear localization sequences.
1995,
Pubmed
Wen,
Identification of a signal for rapid export of proteins from the nucleus.
1995,
Pubmed
Wu,
Nup358, a cytoplasmically exposed nucleoporin with peptide repeats, Ran-GTP binding sites, zinc fingers, a cyclophilin A homologous domain, and a leucine-rich region.
1995,
Pubmed
Yang,
Three-dimensional architecture of the isolated yeast nuclear pore complex: functional and evolutionary implications.
1998,
Pubmed
Yokoyama,
A giant nucleopore protein that binds Ran/TC4.
1995,
Pubmed
You,
Mutations in 5S DNA and 5S RNA have different effects on the binding of Xenopus transcription factor IIIA.
1991,
Pubmed
,
Xenbase
Zasloff,
tRNA transport from the nucleus in a eukaryotic cell: carrier-mediated translocation process.
1983,
Pubmed
,
Xenbase
Zimowska,
A Drosophila Tpr protein homolog is localized both in the extrachromosomal channel network and to nuclear pore complexes.
1997,
Pubmed