Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Virol
1999 Jan 01;731:120-7. doi: 10.1128/JVI.73.1.120-127.1999.
Show Gene links
Show Anatomy links
Nucleoporins nup98 and nup214 participate in nuclear export of human immunodeficiency virus type 1 Rev.
Zolotukhin AS
,
Felber BK
.
???displayArticle.abstract???
Human immunodeficiency virus type 1 (HIV-1) Rev contains a leucine-rich nuclear export signal that is essential for its nucleocytoplasmic export mediated by hCRM1. We examined the role of selected nucleoporins, which are located in peripheral structures of the nuclear pore complex and are thought to be involved in export, in Rev function in human cells. First, we found that upon actinomycin D treatment, Nup98, but not Nup214 or Nup153, is able to translocate to the cytoplasm of HeLa cells, demonstrating that Nup98 may act as a soluble factor. We further showed that Rev can recruit Nup98 and Nup214, but not Nup153, to the nucleolus. We also found that the isolated FG-containing repeat domains of Nup98 and Nup214, but not those of Nup153, competitively inhibit the Rev/RRE-mediated expression of HIV. Taken together, the recruitment of Nup98 and Nup214 by Rev and the competitive inhibition exhibited by their NP domains demonstrate direct participation of Nup98 and Nup214 in the Rev-hCRM1-mediated export.
Arai,
The inv(11)(p15q22) chromosome translocation of de novo and therapy-related myeloid malignancies results in fusion of the nucleoporin gene, NUP98, with the putative RNA helicase gene, DDX10.
1997, Pubmed
Arai,
The inv(11)(p15q22) chromosome translocation of de novo and therapy-related myeloid malignancies results in fusion of the nucleoporin gene, NUP98, with the putative RNA helicase gene, DDX10.
1997,
Pubmed
Bastos,
Targeting and function in mRNA export of nuclear pore complex protein Nup153.
1996,
Pubmed
Boer,
The nucleoporin CAN/Nup214 binds to both the cytoplasmic and the nucleoplasmic sides of the nuclear pore complex in overexpressing cells.
1997,
Pubmed
Boer,
Overexpression of the nucleoporin CAN/NUP214 induces growth arrest, nucleocytoplasmic transport defects, and apoptosis.
1998,
Pubmed
Bogerd,
Identification of a novel cellular cofactor for the Rev/Rex class of retroviral regulatory proteins.
1995,
Pubmed
Borrow,
The t(7;11)(p15;p15) translocation in acute myeloid leukaemia fuses the genes for nucleoporin NUP98 and class I homeoprotein HOXA9.
1996,
Pubmed
Brunet,
Constitutively active mutants of MAP kinase kinase (MEK1) induce growth factor-relaxation and oncogenicity when expressed in fibroblasts.
1994,
Pubmed
Cordes,
Intranuclear filaments containing a nuclear pore complex protein.
1993,
Pubmed
,
Xenbase
Cowley,
Activation of MAP kinase kinase is necessary and sufficient for PC12 differentiation and for transformation of NIH 3T3 cells.
1994,
Pubmed
D'Agostino,
Intracellular trafficking of the human immunodeficiency virus type 1 Rev protein: involvement of continued rRNA synthesis in nuclear retention.
1995,
Pubmed
Fantozzi,
Thermostable inhibitor of cAMP-dependent protein kinase enhances the rate of export of the kinase catalytic subunit from the nucleus.
1994,
Pubmed
Feinberg,
HTLV-III expression and production involve complex regulation at the levels of splicing and translation of viral RNA.
1986,
Pubmed
Fischer,
The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs.
1995,
Pubmed
Fornerod,
CRM1 is an export receptor for leucine-rich nuclear export signals.
1997,
Pubmed
,
Xenbase
Fornerod,
The human homologue of yeast CRM1 is in a dynamic subcomplex with CAN/Nup214 and a novel nuclear pore component Nup88.
1997,
Pubmed
Fritz,
HIV Rev uses a conserved cellular protein export pathway for the nucleocytoplasmic transport of viral RNAs.
1996,
Pubmed
Fritz,
A human nucleoporin-like protein that specifically interacts with HIV Rev.
1995,
Pubmed
Fukuda,
CRM1 is responsible for intracellular transport mediated by the nuclear export signal.
1997,
Pubmed
,
Xenbase
Fukuda,
Interaction of MAP kinase with MAP kinase kinase: its possible role in the control of nucleocytoplasmic transport of MAP kinase.
1997,
Pubmed
Fukuda,
A novel regulatory mechanism in the mitogen-activated protein (MAP) kinase cascade. Role of nuclear export signal of MAP kinase kinase.
1997,
Pubmed
Görlich,
A novel class of RanGTP binding proteins.
1997,
Pubmed
,
Xenbase
Grüter,
TAP, the human homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus.
1998,
Pubmed
,
Xenbase
Izaurralde,
Transport of macromolecules between the nucleus and the cytoplasm.
1998,
Pubmed
Jaaro,
Nuclear translocation of mitogen-activated protein kinase kinase (MEK1) in response to mitogenic stimulation.
1997,
Pubmed
Kraemer,
The human CAN protein, a putative oncogene product associated with myeloid leukemogenesis, is a nuclear pore complex protein that faces the cytoplasm.
1994,
Pubmed
Kutay,
Export of importin alpha from the nucleus is mediated by a specific nuclear transport factor.
1997,
Pubmed
,
Xenbase
Mansour,
Transformation of mammalian cells by constitutively active MAP kinase kinase.
1994,
Pubmed
Mansour,
Constitutively active mitogen-activated protein kinase kinase 1 (MAPKK1) and MAPKK2 mediate similar transcriptional and morphological responses.
1996,
Pubmed
Meyer,
Nuclear transport of human immunodeficiency virus type 1, visna virus, and equine infectious anemia virus Rev proteins: identification of a family of transferable nuclear export signals.
1996,
Pubmed
Nakamura,
Fusion of the nucleoporin gene NUP98 to HOXA9 by the chromosome translocation t(7;11)(p15;p15) in human myeloid leukaemia.
1996,
Pubmed
Neville,
The importin-beta family member Crm1p bridges the interaction between Rev and the nuclear pore complex during nuclear export.
1997,
Pubmed
Nigg,
Nucleocytoplasmic transport: signals, mechanisms and regulation.
1997,
Pubmed
Panté,
Molecular dissection of the nuclear pore complex.
1996,
Pubmed
Pasquinelli,
The constitutive transport element (CTE) of Mason-Pfizer monkey virus (MPMV) accesses a cellular mRNA export pathway.
1997,
Pubmed
,
Xenbase
Powers,
The vertebrate GLFG nucleoporin, Nup98, is an essential component of multiple RNA export pathways.
1997,
Pubmed
,
Xenbase
Powers,
Reconstituted nuclei depleted of a vertebrate GLFG nuclear pore protein, p97, import but are defective in nuclear growth and replication.
1995,
Pubmed
,
Xenbase
Radu,
The peptide repeat domain of nucleoporin Nup98 functions as a docking site in transport across the nuclear pore complex.
1995,
Pubmed
Saavedra,
The simian retrovirus-1 constitutive transport element, unlike the HIV-1 RRE, uses factors required for cellular mRNA export.
1997,
Pubmed
,
Xenbase
Schwartz,
Cloning and functional analysis of multiply spliced mRNA species of human immunodeficiency virus type 1.
1990,
Pubmed
Sodroski,
A second post-transcriptional trans-activator gene required for HTLV-III replication.
,
Pubmed
Solomin,
Different sites of interaction for Rev, Tev, and Rex proteins within the Rev-responsive element of human immunodeficiency virus type 1.
1990,
Pubmed
Stade,
Exportin 1 (Crm1p) is an essential nuclear export factor.
1997,
Pubmed
Stauber,
Development and applications of enhanced green fluorescent protein mutants.
1998,
Pubmed
Stauber,
Analysis of trafficking of Rev and transdominant Rev proteins in living cells using green fluorescent protein fusions: transdominant Rev blocks the export of Rev from the nucleus to the cytoplasm.
1995,
Pubmed
Stutz,
A role for nucleoporin FG repeat domains in export of human immunodeficiency virus type 1 Rev protein and RNA from the nucleus.
1996,
Pubmed
,
Xenbase
Tabernero,
The posttranscriptional control element of the simian retrovirus type 1 forms an extensive RNA secondary structure necessary for its function.
1996,
Pubmed
Towatari,
Constitutive activation of mitogen-activated protein kinase pathway in acute leukemia cells.
1997,
Pubmed
van Deursen,
G2 arrest and impaired nucleocytoplasmic transport in mouse embryos lacking the proto-oncogene CAN/Nup214.
1996,
Pubmed
von Lindern,
Can, a putative oncogene associated with myeloid leukemogenesis, may be activated by fusion of its 3' half to different genes: characterization of the set gene.
1992,
Pubmed
Wen,
Heat-stable inhibitors of cAMP-dependent protein kinase carry a nuclear export signal.
1994,
Pubmed
Wolff,
Leptomycin B is an inhibitor of nuclear export: inhibition of nucleo-cytoplasmic translocation of the human immunodeficiency virus type 1 (HIV-1) Rev protein and Rev-dependent mRNA.
1997,
Pubmed
Zolotukhin,
Continuous propagation of RRE(-) and Rev(-)RRE(-) human immunodeficiency virus type 1 molecular clones containing a cis-acting element of simian retrovirus type 1 in human peripheral blood lymphocytes.
1994,
Pubmed
Zolotukhin,
Mutations in the nuclear export signal of human ran-binding protein RanBP1 block the Rev-mediated posttranscriptional regulation of human immunodeficiency virus type 1.
1997,
Pubmed
