Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
1997 Dec 15;1624:7500-10. doi: 10.1093/emboj/16.24.7500.
Show Gene links
Show Anatomy links
The constitutive transport element (CTE) of Mason-Pfizer monkey virus (MPMV) accesses a cellular mRNA export pathway.
Pasquinelli AE
,
Ernst RK
,
Lund E
,
Grimm C
,
Zapp ML
,
Rekosh D
,
Hammarskjöld ML
,
Dahlberg JE
.
???displayArticle.abstract???
The constitutive transport elements (CTEs) of type D retroviruses are cis-acting elements that promote nuclear export of incompletely spliced mRNAs. Unlike the Rev response element (RRE) of human immunodeficiency virus type 1 (HIV-1), CTEs depend entirely on factors encoded by the host cell genome. We show that an RNA comprised almost entirely of the CTE of Mason-Pfizer monkey virus (CTE RNA) is exported efficiently from Xenopus oocyte nuclei. The CTE RNA and an RNA containing the RRE of HIV-1 (plus Rev) have little effect on export of one another, demonstrating differences in host cell requirements of these two viral mRNA export pathways. Surprisingly, even very low amounts of CTE RNA block export of normal mRNAs, apparently through the sequestration of cellular mRNA export factors. Export of a CTE-containing lariat occurs when wild-type CTE, but not a mutant form, is inserted into the pre-mRNA. The CTE has two symmetric structures, either of which supports export and the titration of mRNA export factors, but both of which are required for maximal inhibition of mRNA export. Two host proteins bind specifically to the CTE but not to non-functional variants, making these proteins candidates for the sequestered mRNA export factors.
Bastos,
Targeting and function in mRNA export of nuclear pore complex protein Nup153.
1996, Pubmed
Bastos,
Targeting and function in mRNA export of nuclear pore complex protein Nup153.
1996,
Pubmed
Bevec,
Inhibition of HIV-1 replication in lymphocytes by mutants of the Rev cofactor eIF-5A.
1996,
Pubmed
Bogerd,
Identification of a novel cellular cofactor for the Rev/Rex class of retroviral regulatory proteins.
1995,
Pubmed
Bray,
A small element from the Mason-Pfizer monkey virus genome makes human immunodeficiency virus type 1 expression and replication Rev-independent.
1994,
Pubmed
Brighty,
A cis-acting repressive sequence that overlaps the Rev-responsive element of human immunodeficiency virus type 1 regulates nuclear retention of env mRNAs independently of known splice signals.
1994,
Pubmed
Chang,
Regulation by HIV Rev depends upon recognition of splice sites.
1989,
Pubmed
Company,
Requirement of the RNA helicase-like protein PRP22 for release of messenger RNA from spliceosomes.
1991,
Pubmed
Dahlberg,
Coupling of nuclear RNA export and protein import in vertebrate cells.
1997,
Pubmed
Daly,
Specific binding of HIV-1 recombinant Rev protein to the Rev-responsive element in vitro.
1989,
Pubmed
Davis,
The nuclear pore complex.
1995,
Pubmed
,
Xenbase
Dignam,
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
1983,
Pubmed
Emerman,
The rev gene product of the human immunodeficiency virus affects envelope-specific RNA localization.
1989,
Pubmed
Ernst,
Secondary structure and mutational analysis of the Mason-Pfizer monkey virus RNA constitutive transport element.
1997,
Pubmed
Ernst,
A structured retroviral RNA element that mediates nucleocytoplasmic export of intron-containing RNA.
1997,
Pubmed
Felber,
rev protein of human immunodeficiency virus type 1 affects the stability and transport of the viral mRNA.
1989,
Pubmed
Fischer,
Evidence that HIV-1 Rev directly promotes the nuclear export of unspliced RNA.
1994,
Pubmed
,
Xenbase
Fischer,
The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs.
1995,
Pubmed
Fritz,
HIV Rev uses a conserved cellular protein export pathway for the nucleocytoplasmic transport of viral RNAs.
1996,
Pubmed
Fritz,
A human nucleoporin-like protein that specifically interacts with HIV Rev.
1995,
Pubmed
Gouilloud,
A dispersed tyrosine tRNA gene from Xenopus laevis with high transcriptional activity in vitro.
1986,
Pubmed
,
Xenbase
Grimm,
In vivo selection of RNAs that localize in the nucleus.
1997,
Pubmed
,
Xenbase
Grimm,
Selection and nuclear immobilization of exportable RNAs.
1997,
Pubmed
,
Xenbase
Hamm,
Monomethylated cap structures facilitate RNA export from the nucleus.
1990,
Pubmed
,
Xenbase
Hammarskjöld,
Regulation of human immunodeficiency virus env expression by the rev gene product.
1989,
Pubmed
Hammarskjöld,
Human immunodeficiency virus env expression becomes Rev-independent if the env region is not defined as an intron.
1994,
Pubmed
Hammarskjöld,
Regulation of retroviral RNA export.
1997,
Pubmed
Huang,
Minimal Rev-response element for type 1 human immunodeficiency virus.
1991,
Pubmed
Izaurralde,
RNA export.
1995,
Pubmed
Izaurralde,
A cap binding protein that may mediate nuclear export of RNA polymerase II-transcribed RNAs.
1992,
Pubmed
,
Xenbase
Izaurralde,
A cap-binding protein complex mediating U snRNA export.
1995,
Pubmed
,
Xenbase
Jarmolowski,
Nuclear export of different classes of RNA is mediated by specific factors.
1994,
Pubmed
,
Xenbase
Kambach,
Intracellular distribution of the U1A protein depends on active transport and nuclear binding to U1 snRNA.
1992,
Pubmed
,
Xenbase
Kjems,
Specific regulation of mRNA splicing in vitro by a peptide from HIV-1 Rev.
1991,
Pubmed
Lee,
Human RNA helicase A is homologous to the maleless protein of Drosophila.
1993,
Pubmed
Legrain,
Some cis- and trans-acting mutants for splicing target pre-mRNA to the cytoplasm.
1989,
Pubmed
Liang,
A DEAD-box-family protein is required for nucleocytoplasmic transport of yeast mRNA.
1996,
Pubmed
Lu,
U1 small nuclear RNA plays a direct role in the formation of a rev-regulated human immunodeficiency virus env mRNA that remains unspliced.
1990,
Pubmed
Lund,
Nonaqueous isolation of transcriptionally active nuclei from Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Malim,
The HIV-1 rev trans-activator acts through a structured target sequence to activate nuclear export of unspliced viral mRNA.
1989,
Pubmed
Malim,
Functional dissection of the HIV-1 Rev trans-activator--derivation of a trans-dominant repressor of Rev function.
1989,
Pubmed
Malim,
HIV-1 structural gene expression requires the binding of multiple Rev monomers to the viral RRE: implications for HIV-1 latency.
1991,
Pubmed
Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed
Meyer,
The HIV-1 Rev trans-activator shuttles between the nucleus and the cytoplasm.
1994,
Pubmed
Nakielny,
Nuclear export of proteins and RNAs.
1997,
Pubmed
Ohno,
A human RNA helicase-like protein, HRH1, facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome.
1996,
Pubmed
Olsen,
Interaction of the human immunodeficiency virus type 1 Rev protein with a structured region in env mRNA is dependent on multimer formation mediated through a basic stretch of amino acids.
1990,
Pubmed
Pasquinelli,
Reverse 5' caps in RNAs made in vitro by phage RNA polymerases.
1995,
Pubmed
,
Xenbase
Perkins,
Structural and functional characterization of the human immunodeficiency virus rev protein.
1989,
Pubmed
Powers,
The vertebrate GLFG nucleoporin, Nup98, is an essential component of multiple RNA export pathways.
1997,
Pubmed
,
Xenbase
Richards,
A nuclear export signal is essential for the cytosolic localization of the Ran binding protein, RanBP1.
1996,
Pubmed
Ruhl,
Eukaryotic initiation factor 5A is a cellular target of the human immunodeficiency virus type 1 Rev activation domain mediating trans-activation.
1993,
Pubmed
,
Xenbase
Saavedra,
The simian retrovirus-1 constitutive transport element, unlike the HIV-1 RRE, uses factors required for cellular mRNA export.
1997,
Pubmed
,
Xenbase
Senapathy,
Splice junctions, branch point sites, and exons: sequence statistics, identification, and applications to genome project.
1990,
Pubmed
Smith,
Two-step affinity purification of U7 small nuclear ribonucleoprotein particles using complementary biotinylated 2'-O-methyl oligoribonucleotides.
1991,
Pubmed
Stutz,
A role for nucleoporin FG repeat domains in export of human immunodeficiency virus type 1 Rev protein and RNA from the nucleus.
1996,
Pubmed
,
Xenbase
Stutz,
Identification of a novel nuclear pore-associated protein as a functional target of the HIV-1 Rev protein in yeast.
1995,
Pubmed
Tabernero,
The posttranscriptional control element of the simian retrovirus type 1 forms an extensive RNA secondary structure necessary for its function.
1996,
Pubmed
Tang,
A cellular cofactor for the constitutive transport element of type D retrovirus.
1997,
Pubmed
Terns,
Retention and 5' cap trimethylation of U3 snRNA in the nucleus.
1994,
Pubmed
,
Xenbase
Terns,
Multiple cis-acting signals for export of pre-U1 snRNA from the nucleus.
1993,
Pubmed
,
Xenbase
van Gelder,
A complex secondary structure in U1A pre-mRNA that binds two molecules of U1A protein is required for regulation of polyadenylation.
1993,
Pubmed
Vankan,
Domains of U4 and U6 snRNAs required for snRNP assembly and splicing complementation in Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Vankan,
Roles of U4 and U6 snRNAs in the assembly of splicing complexes.
1992,
Pubmed
,
Xenbase
Wen,
Identification of a signal for rapid export of proteins from the nucleus.
1995,
Pubmed
Witherell,
Cooperative binding of R17 coat protein to RNA.
1990,
Pubmed
Yeakley,
A complex of nuclear proteins mediates SR protein binding to a purine-rich splicing enhancer.
1996,
Pubmed
Zapp,
Oligomerization and RNA binding domains of the type 1 human immunodeficiency virus Rev protein: a dual function for an arginine-rich binding motif.
1991,
Pubmed
Zapp,
Sequence-specific RNA binding by the HIV-1 Rev protein.
1989,
Pubmed
Zolotukhin,
Continuous propagation of RRE(-) and Rev(-)RRE(-) human immunodeficiency virus type 1 molecular clones containing a cis-acting element of simian retrovirus type 1 in human peripheral blood lymphocytes.
1994,
Pubmed
Zolotukhin,
Mutations in the nuclear export signal of human ran-binding protein RanBP1 block the Rev-mediated posttranscriptional regulation of human immunodeficiency virus type 1.
1997,
Pubmed
