Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Glia
2009 Mar 01;574:380-92. doi: 10.1002/glia.20765.
Show Gene links
Show Anatomy links
Proteomic analysis of the retina: removal of RPE alters outer segment assembly and retinal protein expression.
Wang X
,
Nookala S
,
Narayanan C
,
Giorgianni F
,
Beranova-Giorgianni S
,
McCollum G
,
Gerling I
,
Penn JS
,
Jablonski MM
.
???displayArticle.abstract???
The mechanisms that regulate the complex physiological task of photoreceptor outer segment assembly remain an enigma. One limiting factor in revealing the mechanism(s) by which this process is modulated is that not all of the role players who participate in this process are known. The purpose of this study was to determine some of the retinal proteins that likely play a critical role in regulating photoreceptor outer segment assembly. To do so, we analyzed and compared the proteome map of tadpole Xenopus laevis retinal pigment epithelium (RPE)-supported retinas containing organized outer segments with that of RPE-deprived retinas containing disorganized outer segments. Solubilized proteins were labeled with CyDye fluors followed by multiplexed two-dimensional separation. The intensity of protein spots and comparison of proteome maps was performed using DeCyder software. Identification of differentially regulated proteins was determined using nanoLC-ESI-MS/MS analysis. We found a total of 27 protein spots, 21 of which were unique proteins, which were differentially expressed in retinas with disorganized outer segments. We predict that in the absence of the RPE, oxidative stress initiates an unfolded protein response. Subsequently, downregulation of several candidate Müller glial cell proteins may explain the inability of photoreceptors to properly fold their outer segment membranes. In this study, we have used identification and bioinformatics assessment of proteins that are differentially expressed in retinas with disorganized outer segments as a first step in determining probable key molecules involved in regulating photoreceptor outer segment assembly.
Adler,
Nature and nurture in the differentiation of retinal photoreceptors and neurons.
1987, Pubmed
Adler,
Nature and nurture in the differentiation of retinal photoreceptors and neurons.
1987,
Pubmed
Adler,
Developmental predetermination of the structural and molecular polarization of photoreceptor cells.
1986,
Pubmed
Adler,
Expression of a "survival crisis" by normal and rd/rd mouse photoreceptor cells in vitro.
1989,
Pubmed
Al-Ubaidi,
Proteomics profiling of the cone photoreceptor cell line, 661W.
2008,
Pubmed
Anderson,
Retinal detachment in the cat: the pigment epithelial-photoreceptor interface.
1983,
Pubmed
Araki,
Characterization of photoreceptor cell differentiation in the rat retinal cell culture.
1987,
Pubmed
Bonilha,
Proteomic characterization of isolated retinal pigment epithelium microvilli.
2004,
Pubmed
Caffé,
Histotypic differentiation of neonatal mouse retina in organ culture.
1989,
Pubmed
Cao,
Induction of basic fibroblast growth factor mRNA by basic fibroblast growth factor in Müller cells.
1997,
Pubmed
Chaum,
Retinal neuroprotection by growth factors: a mechanistic perspective.
2003,
Pubmed
Dean,
Constitutive and inducible heat shock protein 70 immunoreactivity in the normal rat eye.
1999,
Pubmed
Diehn,
Differential gene expression in anatomical compartments of the human eye.
2005,
Pubmed
Dryja,
Mutation spectrum of the rhodopsin gene among patients with autosomal dominant retinitis pigmentosa.
1991,
Pubmed
Dryja,
A point mutation of the rhodopsin gene in one form of retinitis pigmentosa.
1990,
Pubmed
Dryja,
Heterozygous missense mutation in the rhodopsin gene as a cause of congenital stationary night blindness.
1993,
Pubmed
Dryja,
Mutations within the rhodopsin gene in patients with autosomal dominant retinitis pigmentosa.
1990,
Pubmed
Farrar,
Autosomal dominant retinitis pigmentosa: linkage to rhodopsin and evidence for genetic heterogeneity.
1990,
Pubmed
Ferrington,
Immunoproteasome responds to injury in the retina and brain.
2008,
Pubmed
Fimbel,
Regeneration of inner retinal neurons after intravitreal injection of ouabain in zebrafish.
2007,
Pubmed
Fliesler,
Chemistry and metabolism of lipids in the vertebrate retina.
1983,
Pubmed
Fokina,
Expression patterns of Wnt genes during development of an anterior part of the chicken eye.
2006,
Pubmed
Gaur,
RPE conditioned medium stimulates photoreceptor cell survival, neurite outgrowth and differentiation in vitro.
1992,
Pubmed
Gerling,
New data analysis and mining approaches identify unique proteome and transcriptome markers of susceptibility to autoimmune diabetes.
2006,
Pubmed
Ghyselinck,
Cellular retinol-binding protein I is essential for vitamin A homeostasis.
1999,
Pubmed
Giorgianni,
LC-MS/MS analysis of peptides with methanol as organic modifier: improved limits of detection.
2004,
Pubmed
Hargrave,
Rhodopsin structure, function, and topography the Friedenwald lecture.
2001,
Pubmed
Hauck,
Proteomic profiling of primary retinal Müller glia cells reveals a shift in expression patterns upon adaptation to in vitro conditions.
2003,
Pubmed
Helle,
Glial and neuronal regulation of the lipid carrier R-FABP.
2003,
Pubmed
Hollyfield,
Pigmented retinal epithelium involvement in photoreceptor development and function.
1974,
Pubmed
Huang,
Downregulation of ATP synthase subunit-6, cytochrome c oxidase-III, and NADH dehydrogenase-3 by bright cyclic light in the rat retina.
2004,
Pubmed
Imamura,
Drusen, choroidal neovascularization, and retinal pigment epithelium dysfunction in SOD1-deficient mice: a model of age-related macular degeneration.
2006,
Pubmed
Ivanov,
Microarray analysis of gene expression in adult retinal ganglion cells.
2006,
Pubmed
Jablonski,
Lactose supports Muller cell protein expression patterns in the absence of the retinal pigment epithelium.
2001,
Pubmed
,
Xenbase
Jablonski,
Lactose promotes organized photoreceptor outer segment assembly and preserves expression of photoreceptor proteins in retinal degeneration.
1999,
Pubmed
,
Xenbase
Jablonski,
Pigment epithelium-derived factor supports normal development of photoreceptor neurons and opsin expression after retinal pigment epithelium removal.
2000,
Pubmed
,
Xenbase
Jablonski,
Closer look at lactose-mediated support of retinal morphogenesis.
2000,
Pubmed
,
Xenbase
Jablonski,
Targeted disruption of Müller cell metabolism induces photoreceptor dysmorphogenesis.
2000,
Pubmed
,
Xenbase
Kijas,
Naturally occurring rhodopsin mutation in the dog causes retinal dysfunction and degeneration mimicking human dominant retinitis pigmentosa.
2002,
Pubmed
Kitamura,
Disruption of the gene encoding the beta1-subunit of transducin in the Rd4/+ mouse.
2006,
Pubmed
Lahav,
In vitro model of retinal photoreceptor differentiation.
1987,
Pubmed
La Vail,
Histotypic organization of the rat retina in vitro.
1971,
Pubmed
Lewis,
Changes in the expression of specific Müller cell proteins during long-term retinal detachment.
1989,
Pubmed
Liang,
Rhodopsin signaling and organization in heterozygote rhodopsin knockout mice.
2004,
Pubmed
Linser,
Induction of glutamine synthetase in embryonic neural retina: localization in Müller fibers and dependence on cell interactions.
1979,
Pubmed
Linser,
Cell contact-dependent regulation of hormonal induction of glutamine synthetase in embryonic neural retina.
1982,
Pubmed
Lupien,
Comparison between the gene expression profile of human Müller cells and two spontaneous Müller cell lines.
2007,
Pubmed
Malhotra,
Endoplasmic reticulum stress and oxidative stress: a vicious cycle or a double-edged sword?
2007,
Pubmed
McWilliam,
Autosomal dominant retinitis pigmentosa (ADRP): localization of an ADRP gene to the long arm of chromosome 3.
1989,
Pubmed
Moscona,
Induction of glutamine synthetase in embryonic neural retina in vitro by inhibitors of macromolecular synthesis.
1970,
Pubmed
Nathans,
Isolation and nucleotide sequence of the gene encoding human rhodopsin.
1984,
Pubmed
Newman,
The Müller cell: a functional element of the retina.
1996,
Pubmed
Papermaster,
Rhodopsin content in the outer segment membranes of bovine and frog retinal rods.
1974,
Pubmed
Paunesku,
Proliferating cell nuclear antigen (PCNA): ringmaster of the genome.
2001,
Pubmed
Piddington,
Correspondence between glutamine synthetase activity and differentiation in the embryonic retina in situ and in cultrue.
1965,
Pubmed
Politi,
Development of neonatal mouse retinal neurons and photoreceptors in low density cell culture.
1988,
Pubmed
Politi,
Effects of docosahexaenoic acid on retinal development: cellular and molecular aspects.
2001,
Pubmed
Prada,
Glutamine synthetase (GS) activity and spatial and temporal patterns of GS expression in the developing chick retina: relationship with synaptogenesis in the outer plexiform layer.
1998,
Pubmed
Reichenbach,
What do retinal müller (glial) cells do for their neuronal 'small siblings'?
1993,
Pubmed
Robinson,
Müller cells in adult rabbit retinae: morphology, distribution and implications for function and development.
1990,
Pubmed
Roderick,
A new dominant retinal degeneration (Rd4) associated with a chromosomal inversion in the mouse.
1997,
Pubmed
Rosenfeld,
A null mutation in the rhodopsin gene causes rod photoreceptor dysfunction and autosomal recessive retinitis pigmentosa.
1992,
Pubmed
Saari,
Biochemistry of visual pigment regeneration: the Friedenwald lecture.
2000,
Pubmed
Saari,
Visual cycle impairment in cellular retinaldehyde binding protein (CRALBP) knockout mice results in delayed dark adaptation.
2001,
Pubmed
Saga,
Development and maintenance of outer segments by isolated chick embryo photoreceptor cells in culture.
1996,
Pubmed
Schröder,
ER stress and the unfolded protein response.
2005,
Pubmed
Sehgal,
Bone morphogenetic protein 7 increases chick photoreceptor outer segment initiation.
2006,
Pubmed
Shaked,
Glutamine synthetase enhances the clearance of extracellular glutamate by the neural retina.
2002,
Pubmed
Sheedlo,
RPE secreted proteins and antibody influence photoreceptor cell survival and maturation.
1998,
Pubmed
Stiemke,
Photoreceptor outer segment development in Xenopus laevis: influence of the pigment epithelium.
1994,
Pubmed
,
Xenbase
Stiemke,
Outer segment disc membrane assembly in the absence of the pigment epithelium: the effect of exogenous sugars.
1994,
Pubmed
,
Xenbase
Sucher,
Molecular basis of glutamate toxicity in retinal ganglion cells.
1997,
Pubmed
Tsai,
Proteomic analysis of human meibomian gland secretions.
2006,
Pubmed
Turner,
A common progenitor for neurons and glia persists in rat retina late in development.
,
Pubmed
Ullian,
Control of synapse number by glia.
2001,
Pubmed
Wahlin,
Neurotrophic factors cause activation of intracellular signaling pathways in Müller cells and other cells of the inner retina, but not photoreceptors.
2000,
Pubmed
Wang,
Contribution of Müller cells toward the regulation of photoreceptor outer segment assembly.
2004,
Pubmed
,
Xenbase
Wang,
Contribution of Müller cells toward the regulation of photoreceptor outer segment assembly.
2011,
Pubmed
,
Xenbase
Watanabe,
Rod photoreceptor development in vitro: intrinsic properties of proliferating neuroepithelial cells change as development proceeds in the rat retina.
1990,
Pubmed
West,
Proteome survey of proliferating and differentiating rat RPE-J cells.
2001,
Pubmed
Whelan,
Light-dependent subcellular movement of photoreceptor proteins.
1988,
Pubmed
Wohabrebbi,
Downregulation of a unique photoreceptor protein correlates with improper outer segment assembly.
2002,
Pubmed
,
Xenbase
Young,
The renewal of photoreceptor cell outer segments.
1967,
Pubmed
