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30 nm chromatin fibre decompaction requires both H4-K16 acetylation and linker histone eviction.
Robinson PJ
,
An W
,
Routh A
,
Martino F
,
Chapman L
,
Roeder RG
,
Rhodes D
.
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The mechanism by which chromatin is decondensed to permit access to DNA is largely unknown. Here, using a model nucleosome array reconstituted from recombinant histone octamers, we have defined the relative contribution of the individual histone octamer N-terminal tails as well as the effect of a targeted histone tail acetylation on the compaction state of the 30 nm chromatin fiber. This study goes beyond previous studies as it is based on a nucleosome array that is very long (61 nucleosomes) and contains a stoichiometric concentration of bound linker histone, which is essential for the formation of the 30 nm chromatin fiber. We find that compaction is regulated in two steps: Introduction of H4 acetylated to 30% on K16 inhibits compaction to a greater degree than deletion of the H4 N-terminal tail. Further decompaction is achieved by removal of the linker histone.
Akhtar,
Activation of transcription through histone H4 acetylation by MOF, an acetyltransferase essential for dosage compensation in Drosophila.
2000, Pubmed
Akhtar,
Activation of transcription through histone H4 acetylation by MOF, an acetyltransferase essential for dosage compensation in Drosophila.
2000,
Pubmed
Allan,
The structure of histone H1 and its location in chromatin.
1980,
Pubmed
Allan,
Participation of core histone "tails" in the stabilization of the chromatin solenoid.
1982,
Pubmed
Allegra,
Affinity chromatographic purification of nucleosomes containing transcriptionally active DNA sequences.
1987,
Pubmed
Allfrey,
Structural Modifications of Histones and their Possible Role in the Regulation of RNA Synthesis.
1964,
Pubmed
An,
Linker histone protects linker DNA on only one side of the core particle and in a sequence-dependent manner.
1998,
Pubmed
An,
Selective requirements for histone H3 and H4 N termini in p300-dependent transcriptional activation from chromatin.
2002,
Pubmed
,
Xenbase
Andersson,
Rapid reformation of the thick chromosome fiber upon completion of RNA synthesis at the Balbiani ring genes in Chironomus tentans.
1982,
Pubmed
Banères,
Evidence indicating proximity in the nucleosome between the histone H4 N termini and the globular domain of histone H1.
1994,
Pubmed
Bashaw,
Dosage compensation and chromatin structure in Drosophila.
1996,
Pubmed
Bates,
Histones H1 and H5: one or two molecules per nucleosome?
1981,
Pubmed
Brownell,
Special HATs for special occasions: linking histone acetylation to chromatin assembly and gene activation.
1996,
Pubmed
Butler,
Changes in chromatin folding in solution.
1980,
Pubmed
Clark,
Electrostatic mechanism of chromatin folding.
1990,
Pubmed
Davey,
DNA-dependent divalent cation binding in the nucleosome core particle.
2002,
Pubmed
Dorigo,
Chromatin fiber folding: requirement for the histone H4 N-terminal tail.
2003,
Pubmed
,
Xenbase
Durrin,
Yeast histone H4 N-terminal sequence is required for promoter activation in vivo.
1991,
Pubmed
Finch,
Solenoidal model for superstructure in chromatin.
1976,
Pubmed
Fletcher,
Core histone tail domains mediate oligonucleosome folding and nucleosomal DNA organization through distinct molecular mechanisms.
1995,
Pubmed
Huynh,
A method for the in vitro reconstitution of a defined "30 nm" chromatin fibre containing stoichiometric amounts of the linker histone.
2005,
Pubmed
Jenuwein,
Translating the histone code.
2001,
Pubmed
Kimura,
Chromosomal gradient of histone acetylation established by Sas2p and Sir2p functions as a shield against gene silencing.
2002,
Pubmed
Kornberg,
Structure of chromatin.
1977,
Pubmed
Kouzarides,
Chromatin modifications and their function.
2007,
Pubmed
Krajewski,
Modulation of the higher-order folding of chromatin by deletion of histone H3 and H4 terminal domains.
1996,
Pubmed
Langmore,
The higher order structure of chicken erythrocyte chromosomes in vivo.
1980,
Pubmed
Li,
The role of chromatin during transcription.
2007,
Pubmed
Lowary,
New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning.
1998,
Pubmed
Luger,
Crystal structure of the nucleosome core particle at 2.8 A resolution.
1997,
Pubmed
Marsden,
Metaphase chromosome structure: evidence for a radial loop model.
1979,
Pubmed
Mizzen,
Transcription. New insights into an old modification.
2000,
Pubmed
Morales,
Functional integration of the histone acetyltransferase MOF into the dosage compensation complex.
2004,
Pubmed
Noll,
Action of micrococcal nuclease on chromatin and the location of histone H1.
1977,
Pubmed
Philo,
Improved methods for fitting sedimentation coefficient distributions derived by time-derivative techniques.
2006,
Pubmed
Richmond,
Structure of the nucleosome core particle at 7 A resolution.
,
Pubmed
Robinson,
Structure of the '30 nm' chromatin fibre: a key role for the linker histone.
2006,
Pubmed
Robinson,
EM measurements define the dimensions of the "30-nm" chromatin fiber: evidence for a compact, interdigitated structure.
2006,
Pubmed
Schalch,
X-ray structure of a tetranucleosome and its implications for the chromatin fibre.
2005,
Pubmed
,
Xenbase
Seet,
Reading protein modifications with interaction domains.
2006,
Pubmed
Shia,
Histone H4 lysine 16 acetylation breaks the genome's silence.
2006,
Pubmed
Shogren-Knaak,
Histone H4-K16 acetylation controls chromatin structure and protein interactions.
2006,
Pubmed
Smith,
Mass spectrometric quantification of acetylation at specific lysines within the amino-terminal tail of histone H4.
2003,
Pubmed
Smith,
Linking global histone acetylation to the transcription enhancement of X-chromosomal genes in Drosophila males.
2001,
Pubmed
Stafford,
Boundary analysis in sedimentation transport experiments: a procedure for obtaining sedimentation coefficient distributions using the time derivative of the concentration profile.
1992,
Pubmed
Suka,
Sir2p and Sas2p opposingly regulate acetylation of yeast histone H4 lysine16 and spreading of heterochromatin.
2002,
Pubmed
Sun,
Electrostatic mechanism of nucleosomal array folding revealed by computer simulation.
2005,
Pubmed
Thoma,
Involvement of histone H1 in the organization of the nucleosome and of the salt-dependent superstructures of chromatin.
1979,
Pubmed
Thomas,
Histone H1: location and role.
1999,
Pubmed
Tse,
Disruption of higher-order folding by core histone acetylation dramatically enhances transcription of nucleosomal arrays by RNA polymerase III.
1998,
Pubmed
,
Xenbase
Turner,
Histone acetylation and an epigenetic code.
2000,
Pubmed
van Holde,
What determines the folding of the chromatin fiber?
1996,
Pubmed
Widom,
A relationship between the helical twist of DNA and the ordered positioning of nucleosomes in all eukaryotic cells.
1992,
Pubmed
Widom,
Structure of the 300A chromatin filament: X-ray diffraction from oriented samples.
1985,
Pubmed
Woodcock,
Chromatin architecture.
2006,
Pubmed
