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???displayArticle.abstract??? Pancreas-specific transcription factor 1a (Ptf1a), a bHLH transcription factor, has two temporally distinct functions during pancreas development; initially it is required for early specification of the entire pancreas, while later it is required for proper differentiation and maintenance of only acinar cells. The importance of Ptf1a function was revealed by the fact that loss of Ptf1a leads to pancreas agenesis in humans. While Ptf1a is one of the most important pancreatic transcription factors, little is known about the differences between the regulatory networks it controls during initial specification of the pancreas as opposed to acinar cell development, and to date no comprehensive analysis of its downstream targets has been published. In this article, we use Xenopus embryos to identify putative downstream targets of Ptf1a. We isolated anterior endodermtissue overexpressing Ptf1a at two early stages, NF32 and NF36, and compared their gene expression profiles using microarrays. Our results revealed that Ptf1a regulates genes with a wide variety of functions, providing insight into the complexity of the regulatory network required for pancreas specification.
Figure 2. RT-PCR verification of selected differentially expressed genes from the microarray analyses. GFP and Ptf1a sample data provided from three different cycle time-points. GFP, gfp mRNA-injected control; Ptf1a, ptf1a mRNA-injected; -RT, control reaction without reverse transcriptase. Reproduced with permission of the Publisher, John Wiley & Sons.
irf1 (interferon regulatory factor 1 ) gene expression in dissected Xenopus laevis gut , assayed via in situ hybridization, NF stage 44.
stxbp1 (syntaxin binding protein 1 ) gene expression in dissected Xenopus laevis gut, assayed via in situ hybridization, NF stage 44.
stx1b (syntaxin 1B ) gene expression in dissected Xenopus laevis gut, assayed via in situ hybridization, NF stage 44.
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