Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
2000 Dec 15;1924:6860-9. doi: 10.1093/emboj/19.24.6860.
Show Gene links
Show Anatomy links
The spliceosome deposits multiple proteins 20-24 nucleotides upstream of mRNA exon-exon junctions.
Le Hir H
,
Izaurralde E
,
Maquat LE
,
Moore MJ
.
???displayArticle.abstract???
Eukaryotic mRNAs exist in vivo as ribonucleoprotein particles (mRNPs). The protein components of mRNPs have important functions in mRNA metabolism, including effects on subcellular localization, translational efficiency and mRNA half-life. There is accumulating evidence that pre-mRNA splicing can alter mRNP structure and thereby affect downstream mRNA metabolism. Here, we report that the spliceosome stably deposits several proteins on mRNAs, probably as a single complex of approximately 335 kDa. This complex protects 8 nucleotides of mRNA from complete RNase digestion at a conserved position 20-24 nucleotides upstream of exon-exon junctions. Splicing-dependent RNase protection of this region was observed in both HeLa cell nuclear extracts and Xenopus laevis oocyte nuclei. Immunoprecipitations revealed that five components of the complex are the splicing-associated factors SRm160, DEK and RNPS1, the mRNA-associated shuttling protein Y14 and the mRNA export factor REF. Possible functions for this complex in nucleocytoplasmic transport of spliced mRNA, as well as the nonsense-mediated mRNA decay pathway, are discussed.
Alexiadis,
The protein encoded by the proto-oncogene DEK changes the topology of chromatin and reduces the efficiency of DNA replication in a chromatin-specific manner.
2000, Pubmed
Alexiadis,
The protein encoded by the proto-oncogene DEK changes the topology of chromatin and reduces the efficiency of DNA replication in a chromatin-specific manner.
2000,
Pubmed
Bachi,
The C-terminal domain of TAP interacts with the nuclear pore complex and promotes export of specific CTE-bearing RNA substrates.
2000,
Pubmed
,
Xenbase
Bear,
Identification of novel import and export signals of human TAP, the protein that binds to the constitutive transport element of the type D retrovirus mRNAs.
1999,
Pubmed
,
Xenbase
Blencowe,
A coactivator of pre-mRNA splicing.
1998,
Pubmed
Blencowe,
The SRm160/300 splicing coactivator subunits.
2000,
Pubmed
Blencowe,
Association of nuclear matrix antigens with exon-containing splicing complexes.
1994,
Pubmed
Braun,
TAP binds to the constitutive transport element (CTE) through a novel RNA-binding motif that is sufficient to promote CTE-dependent RNA export from the nucleus.
1999,
Pubmed
,
Xenbase
Burd,
RNA binding specificity of hnRNP A1: significance of hnRNP A1 high-affinity binding sites in pre-mRNA splicing.
1994,
Pubmed
Chiara,
Identification of proteins that interact with exon sequences, splice sites, and the branchpoint sequence during each stage of spliceosome assembly.
1996,
Pubmed
Collins,
Allele-specific genetic interactions between Prp8 and RNA active site residues suggest a function for Prp8 at the catalytic core of the spliceosome.
1999,
Pubmed
Dreyfuss,
hnRNP proteins and the biogenesis of mRNA.
1993,
Pubmed
Duchêne,
Molecular consequences of truncations of the first exon for in vitro splicing of yeast actin pre-mRNA.
1988,
Pubmed
Eldridge,
The SRm160/300 splicing coactivator is required for exon-enhancer function.
1999,
Pubmed
Fortes,
The yeast nuclear cap binding complex can interact with translation factor eIF4G and mediate translation initiation.
2000,
Pubmed
Görlach,
The mRNA poly(A)-binding protein: localization, abundance, and RNA-binding specificity.
1994,
Pubmed
Gozani,
Evidence that sequence-independent binding of highly conserved U2 snRNP proteins upstream of the branch site is required for assembly of spliceosomal complex A.
1996,
Pubmed
Gozani,
A novel set of spliceosome-associated proteins and the essential splicing factor PSF bind stably to pre-mRNA prior to catalytic step II of the splicing reaction.
1994,
Pubmed
Grüter,
TAP, the human homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus.
1998,
Pubmed
,
Xenbase
Günzl,
Oligonucleotide-targeted RNase H protection analysis of RNA-protein complexes.
1999,
Pubmed
Hentze,
A perfect message: RNA surveillance and nonsense-mediated decay.
1999,
Pubmed
Izaurralde,
A cap-binding protein complex mediating U snRNA export.
1995,
Pubmed
,
Xenbase
Jarmolowski,
Nuclear export of different classes of RNA is mediated by specific factors.
1994,
Pubmed
,
Xenbase
Katahira,
The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human.
1999,
Pubmed
Kataoka,
Pre-mRNA splicing imprints mRNA in the nucleus with a novel RNA-binding protein that persists in the cytoplasm.
2000,
Pubmed
,
Xenbase
Krause,
Immunodetection of poly(A) binding protein II in the cell nucleus.
1994,
Pubmed
Le Hir,
Pre-mRNA splicing alters mRNP composition: evidence for stable association of proteins at exon-exon junctions.
2000,
Pubmed
Li,
Nonsense surveillance in lymphocytes?
1998,
Pubmed
Luo,
Splicing is required for rapid and efficient mRNA export in metazoans.
1999,
Pubmed
,
Xenbase
Luo,
The human Prp8 protein is a component of both U2- and U12-dependent spliceosomes.
1999,
Pubmed
Maquat,
When cells stop making sense: effects of nonsense codons on RNA metabolism in vertebrate cells.
1995,
Pubmed
Mayeda,
Purification and characterization of human RNPS1: a general activator of pre-mRNA splicing.
1999,
Pubmed
McGarvey,
The acute myeloid leukemia-associated protein, DEK, forms a splicing-dependent interaction with exon-product complexes.
2000,
Pubmed
Nagy,
A rule for termination-codon position within intron-containing genes: when nonsense affects RNA abundance.
1998,
Pubmed
Nakielny,
Transport of proteins and RNAs in and out of the nucleus.
1999,
Pubmed
Nakielny,
The hnRNP C proteins contain a nuclear retention sequence that can override nuclear export signals.
1996,
Pubmed
Query,
Branch nucleophile selection in pre-mRNA splicing: evidence for the bulged duplex model.
1994,
Pubmed
Reed,
The role of the mammalian branchpoint sequence in pre-mRNA splicing.
1988,
Pubmed
Sachs,
Eukaryotic translation initiation: there are (at least) two sides to every story.
2000,
Pubmed
Shyu,
The double lives of shuttling mRNA binding proteins.
2000,
Pubmed
Siatecka,
Functional interactions of Prp8 with both splice sites at the spliceosomal catalytic center.
1999,
Pubmed
Strässer,
Yra1p, a conserved nuclear RNA-binding protein, interacts directly with Mex67p and is required for mRNA export.
2000,
Pubmed
Stutz,
REF, an evolutionary conserved family of hnRNP-like proteins, interacts with TAP/Mex67p and participates in mRNA nuclear export.
2000,
Pubmed
,
Xenbase
Sun,
Nonsense-mediated decay of glutathione peroxidase 1 mRNA in the cytoplasm depends on intron position.
2000,
Pubmed
Teigelkamp,
Extensive interactions of PRP8 protein with the 5' and 3' splice sites during splicing suggest a role in stabilization of exon alignment by U5 snRNA.
1995,
Pubmed
Thermann,
Binary specification of nonsense codons by splicing and cytoplasmic translation.
1998,
Pubmed
Visa,
A nuclear cap-binding complex binds Balbiani ring pre-mRNA cotranscriptionally and accompanies the ribonucleoprotein particle during nuclear export.
1996,
Pubmed
Wahle,
A novel poly(A)-binding protein acts as a specificity factor in the second phase of messenger RNA polyadenylation.
1991,
Pubmed
Zhang,
At least one intron is required for the nonsense-mediated decay of triosephosphate isomerase mRNA: a possible link between nuclear splicing and cytoplasmic translation.
1998,
Pubmed
Zhang,
Intron function in the nonsense-mediated decay of beta-globin mRNA: indications that pre-mRNA splicing in the nucleus can influence mRNA translation in the cytoplasm.
1998,
Pubmed
Zhou,
The protein Aly links pre-messenger-RNA splicing to nuclear export in metazoans.
2000,
Pubmed
