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Insect Biochem Mol Biol
2008 May 01;385:604-10. doi: 10.1016/j.ibmb.2008.01.003.
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Molecular and functional characterization of voltage-gated sodium channel variants from Drosophila melanogaster.
Olson RO
,
Liu Z
,
Nomura Y
,
Song W
,
Dong K
.
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Extensive alternative splicing and RNA editing have been documented for the transcript of DmNa(V) (formerly para), the sole sodium channel gene in Drosophila melanogaster. However, the functional consequences of these post-transcriptional modifications are not well understood. In this study we isolated 64 full-length DmNa(V) cDNA clones from D. melanogaster adults. Based on the usage of 11 alternative exons, 64 clones could be grouped into 29 splice types. When expressed in Xenopus oocytes, 33 DmNa(V) variants generated sodium currents large enough for functional characterization. Among these variants, DmNa(V)5-1 and DmNa(V)7-1 channels activated at the most hyperpolarizing potentials, whereas DmNa(V)1-6 and DmNa(V)19 channels activated at the most depolarizing membrane potentials. We identified an A-to-I editing event in DmNa(V)5-1 that is responsible for its uniquely low-voltage-dependent activation. The wide range of voltage dependence of gating properties exhibited by DmNa(V) variants represents a rich resource for future studies to determine the role of DmNa(V) in regulating sodium channel gating, pharmacology, and neuronal excitability in insects.
Byerly,
Ionic currents of Drosophila neurons in embryonic cultures.
1988, Pubmed
Byerly,
Ionic currents of Drosophila neurons in embryonic cultures.
1988,
Pubmed
Catterall,
From ionic currents to molecular mechanisms: the structure and function of voltage-gated sodium channels.
2000,
Pubmed
Defaix,
Role of a novel maintained low-voltage-activated inward current permeable to sodium and calcium in pacemaking of insect neurosecretory neurons.
2005,
Pubmed
Derst,
Four novel sequences in Drosophila melanogaster homologous to the auxiliary Para sodium channel subunit TipE.
2006,
Pubmed
,
Xenbase
Dong,
Insect sodium channels and insecticide resistance.
2007,
Pubmed
,
Xenbase
Feng,
Cloning and functional analysis of TipE, a novel membrane protein that enhances Drosophila para sodium channel function.
1995,
Pubmed
,
Xenbase
Goldin,
Nomenclature of voltage-gated sodium channels.
2000,
Pubmed
Grolleau,
Dorsal unpaired median neurones in the insect central nervous system: towards a better understanding of the ionic mechanisms underlying spontaneous electrical activity.
2000,
Pubmed
Lapied,
Biophysical properties of scorpion alpha-toxin-sensitive background sodium channel contributing to the pacemaker activity in insect neurosecretory cells (DUM neurons).
1999,
Pubmed
Le Corronc,
Ionic mechanisms underlying depolarizing responses of an identified insect motor neuron to short periods of hypoxia.
1999,
Pubmed
Lee,
Developmental regulation of alternative exon usage in the house fly Vssc1 sodium channel gene.
2002,
Pubmed
Liu,
Persistent tetrodotoxin-sensitive sodium current resulting from U-to-C RNA editing of an insect sodium channel.
2004,
Pubmed
Loughney,
Molecular analysis of the para locus, a sodium channel gene in Drosophila.
1989,
Pubmed
O'Dowd,
Voltage-gated currents and firing properties of embryonic Drosophila neurons grown in a chemically defined medium.
1995,
Pubmed
O'Dowd,
Voltage-clamp analysis of sodium channels in wild-type and mutant Drosophila neurons.
1988,
Pubmed
Palladino,
A-to-I pre-mRNA editing in Drosophila is primarily involved in adult nervous system function and integrity.
2000,
Pubmed
Plummer,
Alternative splicing of the sodium channel SCN8A predicts a truncated two-domain protein in fetal brain and non-neuronal cells.
1997,
Pubmed
Saito,
Ionic channels in cultured Drosophila neurons.
1993,
Pubmed
Saito,
Expression of ion channels and mutational effects in giant Drosophila neurons differentiated from cell division-arrested embryonic neuroblasts.
1991,
Pubmed
Schäfer,
Ionic currents of Kenyon cells from the mushroom body of the honeybee.
1994,
Pubmed
Song,
RNA editing generates tissue-specific sodium channels with distinct gating properties.
2004,
Pubmed
,
Xenbase
Tan,
Alternative splicing of an insect sodium channel gene generates pharmacologically distinct sodium channels.
2002,
Pubmed
,
Xenbase
Thackeray,
Developmentally regulated alternative splicing generates a complex array of Drosophila para sodium channel isoforms.
1994,
Pubmed
Thackeray,
Conserved alternative splicing patterns and splicing signals in the Drosophila sodium channel gene para.
1995,
Pubmed
Wang,
Molecular characterization of an arachnid sodium channel gene from the varroa mite (Varroa destructor).
2003,
Pubmed
Warmke,
Functional expression of Drosophila para sodium channels. Modulation by the membrane protein TipE and toxin pharmacology.
1997,
Pubmed
,
Xenbase
Wicher,
Non-synaptic ion channels in insects--basic properties of currents and their modulation in neurons and skeletal muscles.
2001,
Pubmed
Yu,
Overview of the voltage-gated sodium channel family.
2003,
Pubmed
