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Fidelity of transcription of Xenopus laevis globin genes injected into Xenopus laevis oocytes and unfertilized eggs.
Bendig MM
,
Williams JG
.
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The Xenopus laevis alpha 1- and beta 1-globin genes were injected into oocytes and unfertilized eggs of X. laevis. In oocytes, the injected globin genes were actively transcribed, but the majority of the transcripts were incorrectly initiated. In unfertilized eggs, the injected genes were transcribed at a low level but only from the correct start sites. In oocytes, the injected circular plasmid DNA containing the cloned globin genes persisted but did not replicate. In contrast, DNA injected into unfertilized eggs replicated up to 15-fold within a 22-h period. We suggest that the ability of the egg to selectively transcribe the injected X. laevis globin genes from the correct promoter sites may be related to differences in chromatin structure between the oocyte and the unfertilized egg.
Bendig,
Persistence and expression of histone genes injected into Xenopus eggs in early development.
1981, Pubmed,
Xenbase
Bendig,
Persistence and expression of histone genes injected into Xenopus eggs in early development.
1981,
Pubmed
,
Xenbase
Bendig,
Differential expression of the Xenopus laevis tadpole and adult beta-globin genes when injected into fertilized Xenopus laevis eggs.
1984,
Pubmed
,
Xenbase
Bendig,
Replication and expression of Xenopus laevis globin genes injected into fertilized Xenopus eggs.
1983,
Pubmed
,
Xenbase
Busby,
Fate of amplified nucleoli in Xenopus laevis embryos.
1982,
Pubmed
,
Xenbase
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Etkin,
The synthesis of authentic sea urchin transcriptional and translational products by sea urchin histone genes injected into Xenopus laevis oocytes.
1980,
Pubmed
,
Xenbase
Ford,
DNA synthesis in ocytes and eggs of Xenopus laevis injected with DNA.
1975,
Pubmed
,
Xenbase
Gargiulo,
Properties of the chromatin assembled on DNA injected into Xenopus oocytes and eggs.
1983,
Pubmed
,
Xenbase
Gurdon,
Gene transfer in amphibian eggs and oocytes.
1981,
Pubmed
Gurdon,
The replication of purified DNA introduced into living egg cytoplasm.
1969,
Pubmed
Harland,
Transcription of DNA injected into Xenopus oocytes is influenced by template topology.
1983,
Pubmed
,
Xenbase
Harland,
Regulated replication of DNA microinjected into eggs of Xenopus laevis.
1980,
Pubmed
,
Xenbase
Igo-Kemenes,
Chromatin.
1982,
Pubmed
Ikegami,
Aphidicolin prevents mitotic cell division by interfering with the activity of DNA polymerase-alpha.
1978,
Pubmed
Jeffreys,
Linkage of adult alpha- and beta-globin genes in X. laevis and gene duplication by tetraploidization.
1980,
Pubmed
,
Xenbase
Jones,
Regulation of adenovirus transcription by an E1a gene in microinjected Xenopus laevis oocytes.
1983,
Pubmed
,
Xenbase
Korn,
Oocyte extracts reactivate developmentally inert Xenopus 5S genes in somatic nuclei.
1982,
Pubmed
,
Xenbase
Laskey,
Induction of chromosome replication during maturation of amphibian oocytes.
1983,
Pubmed
,
Xenbase
Laskey,
Chromatin assembly and transcription in eggs and oocytes of Xenopus laevis.
1978,
Pubmed
,
Xenbase
Laskey,
Induction of polyoma DNA synthesis by injection into frog-egg cytoplasm.
1973,
Pubmed
,
Xenbase
McKnight,
Analysis of transcriptional regulatory signals of the HSV thymidine kinase gene: identification of an upstream control region.
1981,
Pubmed
,
Xenbase
Méchali,
DNA synthesis in a cell-free system from Xenopus eggs: priming and elongation on single-stranded DNA in vitro.
1982,
Pubmed
,
Xenbase
Mertz,
Purified DNAs are transcribed after microinjection into Xenopus oocytes.
1977,
Pubmed
,
Xenbase
Miller,
Template structural requirements for transcription in vivo by RNA polymerase II.
1982,
Pubmed
,
Xenbase
Newport,
A major developmental transition in early Xenopus embryos: II. Control of the onset of transcription.
1982,
Pubmed
,
Xenbase
Newport,
A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
1982,
Pubmed
,
Xenbase
Patient,
Internal organization of the major adult alpha- and beta-globin genes of X. laevis.
1980,
Pubmed
,
Xenbase
Patient,
The complete nucleotide sequence of the major adult beta globin gene of Xenopus laevis.
1983,
Pubmed
,
Xenbase
Rusconi,
Transformation of frog embryos with a rabbit beta-globin gene.
1981,
Pubmed
,
Xenbase
Ryoji,
Chromatin assembly in Xenopus oocytes: in vivo studies.
1984,
Pubmed
,
Xenbase
Twigg,
Trans-complementable copy-number mutants of plasmid ColE1.
1980,
Pubmed
Wallace,
Protein incorporation by isolated amphibian oocytes. 3. Optimum incubation conditions.
1973,
Pubmed
,
Xenbase
Wickens,
Expression of a chicken chromosomal ovalbumin gene injected into frog oocyte nuclei.
1980,
Pubmed
,
Xenbase
Wyllie,
Selective DNA conservation and chromatin assembly after injection of SV40 DNA into Xenopus oocytes.
1978,
Pubmed
,
Xenbase