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In Vitro Cell Dev Biol
1987 May 01;235:323-31. doi: 10.1007/bf02620988.
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The effects of various nutritional supplements on the growth, migration and differentiation of Xenopus laevis neural crest cells in vitro.
Wilson HC
,
Milos NC
.
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This study investigates the nutritional requirements of Xenopus laevis neural crest cells and melanophores developing in vitro. A comparison is made between the growth and differentiation of cells in serum-containing medium and a chemically defined, serum-free medium that we have designed. Our chemically defined medium is more efficient than serum-supplemented medium in promoting proliferation of these cells. Several supplements are required to enhance culture development. These include insulin, alpha-melanocyte stimulating hormone, somatotropin, luteotrophic hormone, linoleic acid, uridine, and putrescine. In addition, collagen and fibronectin provide the most conducive environment tested for cell migration and adhesion.
Andrew,
Hanging drop culture of Xenopus laevis neural crest.
1969, Pubmed,
Xenbase
Andrew,
Hanging drop culture of Xenopus laevis neural crest.
1969,
Pubmed
,
Xenbase
Barbehenn,
An examination of the efficiency of glucose and glutamine as energy sources for cultured chick pigment epithelial cells.
1984,
Pubmed
Barnes,
Methods for growth of cultured cells in serum-free medium.
1980,
Pubmed
Bregman,
Anchorage-independent growth of murine melanoma in serum-less media is dependent on insulin or melanocyte-stimulating hormone.
1985,
Pubmed
Chen,
Development of cell surface linkage complexes in cultured fibroblasts.
1985,
Pubmed
Duband,
Distribution of fibronectin in the early phase of avian cephalic neural crest cell migration.
1982,
Pubmed
Erickson,
Substrata formed by combinations of extracellular matrix components alter neural crest cell motility in vitro.
1983,
Pubmed
Erickson,
Control of neural crest cell dispersion in the trunk of the avian embryo.
1985,
Pubmed
Gabie,
Staging of pigment cells in cultures of Xenopus laevis neural crest.
1969,
Pubmed
,
Xenbase
Gail,
The locomotion of mouse fibroblasts in tissue culture.
1970,
Pubmed
Gilchrest,
Substrate influences human epidermal melanocyte attachment and spreading in vitro.
1985,
Pubmed
Goodman,
Do cells show an inverse locomotory response to fibronectin and laminin substrates?
1985,
Pubmed
Greenberg,
Role of collagen and fibronectin in neural crest cell adhesion and migration.
1981,
Pubmed
Ham,
Putrescine and related amines as growth factors for a mammalian cell line.
1964,
Pubmed
Heby,
Role of polyamines in the control of cell proliferation and differentiation.
1981,
Pubmed
Ide,
Proliferation of amphibian melanophores in vitro.
1974,
Pubmed
Keller,
Neural crest cell behavior in white and dark larvae of Ambystoma mexicanum: time-lapse cinemicrographic analysis of pigment cell movement in vivo and in culture.
1984,
Pubmed
Kleinman,
Biological activities of laminin.
1985,
Pubmed
Kondo,
Long-term cultivation of amphibian melanophores. In vitro ageing and spontaneous transformation to a continuous cell line.
1983,
Pubmed
Laterra,
Cell surface heparan sulfate mediates some adhesive responses to glycosaminoglycan-binding matrices, including fibronectin.
1983,
Pubmed
Le Douarin,
Migration and differentiation of neural crest cells.
1980,
Pubmed
Loring,
Extracellular matrix materials influence quail neural crest cell differentiation in vitro.
1982,
Pubmed
Mather,
Ceruloplasmin, a copper-transport protein, can act as a growth promoter for some cell lines in serum-free medium.
1982,
Pubmed
Mather,
The growth of mouse melanoma cells in hormone-supplemented, serum-free medium.
1979,
Pubmed
Matsuda,
Studies on the development of melanophores in in vitro cultured amphibian neural plates.
1983,
Pubmed
McKeehan,
Stimulation of clonal growth of normal fibroblasts with substrata coated with basic polymers.
1976,
Pubmed
McKeehan,
Selenium is an essential trace nutrient for growth of WI-38 diploid human fibroblasts.
1976,
Pubmed
Milos,
Cell surface carbohydrate involvement in controlling the adhesion and morphology of neural crest cells and melanophores of Xenopus laevis.
1986,
Pubmed
,
Xenbase
Milos,
Changes in the utilization of cell surface carbohydrates are implicated in the adhesion of Xenopus laevis melanophores in vitro.
1986,
Pubmed
,
Xenbase
Morell,
Physical and chemical studies on ceruloplasmin. IV. Preparation of radioactive, sialic acid-free ceruloplasmin labeled with tritium on terminal D-galactose residues.
1966,
Pubmed
Oka,
A serum-free defined medium for retinal pigment epithelial cells.
1984,
Pubmed
Rovasio,
Neural crest cell migration: requirements for exogenous fibronectin and high cell density.
1983,
Pubmed
Ruoslahti,
Alignment of biologically active domains in the fibronectin molecule.
1981,
Pubmed
Sakashita,
Basement membrane glycoprotein laminin binds to heparin.
1980,
Pubmed
Seldenrijk,
Morphological and physiological aspects of melanophores in primary culture from tadpoles of Xenopus laevis.
1979,
Pubmed
,
Xenbase
Sieber-Blum,
Cellular fibronectin promotes adrenergic differentiation of quail neural crest cells in vitro.
1981,
Pubmed
Sieber-Blum,
In vitro proliferation and terminal differentiation of quail neural crest cells in a defined culture medium.
1985,
Pubmed
Smith,
Localization of two unique heparin binding domains of human plasma fibronectin with monoclonal antibodies.
1982,
Pubmed
Srivenugopal,
Differential modulation by spermidine of reactions catalyzed by type 1 prokaryotic and eukaryotic topoisomerases.
1985,
Pubmed
Terranova,
Laminin receptor on human breast carcinoma cells.
1983,
Pubmed
Thorogood,
Effects of vitamin A on the behaviour of migratory neural crest cells in vitro.
1982,
Pubmed
Tickle,
Observations on nudging cells in culture.
1976,
Pubmed
Tjälve,
Affinity of putrescine, spermidine and spermine for pigmented tissues.
1982,
Pubmed
TWITTY,
Causal analysis of chromatophore migration.
1948,
Pubmed
Wion,
Serum and thyroid hormones T3 and T4 regulate nerve growth factor mRNA levels in mouse L cells.
1985,
Pubmed
Wittenberg,
A detergent-activated tyrosinase from Xenopus laevis. I. Purification and partial characterization.
1985,
Pubmed
,
Xenbase
Ziller,
Early segregation of a neuronal precursor cell line in the neural crest as revealed by culture in a chemically defined medium.
1983,
Pubmed
