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Transcription of cloned tRNA and 5S RNA genes in a Drosophila cell free extract.
Dingermann T
,
Sharp S
,
Appel B
,
DeFranco D
,
Mount S
,
Heiermann R
,
Pongs O
,
Söll D
.
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We describe the preparation of a cell-free extract from Drosophila Kc cells which allows transcription of a variety of cloned eukaryotic RNA polymerase III genes. The extract has low RNA-processing nuclease activity and thus the major products obtained are primary transcripts.
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