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???displayArticle.abstract??? Maturation-promoting factor (MPF) is a cell cycle control element able to cause cells to enter M-phase upon microinjection and will induce metaphase in nuclei incubated in cell extracts. Previous work has shown that MPF is composed of a complex between p34cdc 2 protein kinase and a B-type cyclin. In the present work gamma-S-ATP was found to cause activation of MPF activity in partially purified preparations, but this activation was lost upon chromatography on Matrex Green gel A. Readdition of other Matrex Green fractions to purified MPF restored the ability of gamma-S-ATP to activate MPF for nuclear breakdown as well as phosphorylation of histone H1. Use of the system described here will facilitate study of p34cdc 2 kinase activation and identification of elements involved in MPF regulation.
Arion,
cdc2 is a component of the M phase-specific histone H1 kinase: evidence for identity with MPF.
1988, Pubmed,
Xenbase
Arion,
cdc2 is a component of the M phase-specific histone H1 kinase: evidence for identity with MPF.
1988,
Pubmed
,
Xenbase
Booher,
Involvement of cdc13+ in mitotic control in Schizosaccharomyces pombe: possible interaction of the gene product with microtubules.
1988,
Pubmed
Cyert,
Regulation of MPF activity in vitro.
1988,
Pubmed
,
Xenbase
Cyert,
Monoclonal antibodies specific for thiophosphorylated proteins recognize Xenopus MPF.
1988,
Pubmed
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Xenbase
Draetta,
Activation of cdc2 protein kinase during mitosis in human cells: cell cycle-dependent phosphorylation and subunit rearrangement.
1988,
Pubmed
Dunphy,
Fission yeast p13 blocks mitotic activation and tyrosine dephosphorylation of the Xenopus cdc2 protein kinase.
1989,
Pubmed
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Xenbase
Dunphy,
Mitosis-inducing factors are present in a latent form during interphase in the Xenopus embryo.
1988,
Pubmed
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Xenbase
Dunphy,
The Xenopus cdc2 protein is a component of MPF, a cytoplasmic regulator of mitosis.
1988,
Pubmed
,
Xenbase
Erikson,
Biochemical characterization of the p34cdc2 protein kinase component of purified maturation-promoting factor from Xenopus eggs.
1989,
Pubmed
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Xenbase
Evans,
Cyclin: a protein specified by maternal mRNA in sea urchin eggs that is destroyed at each cleavage division.
1983,
Pubmed
Gautier,
Cyclin is a component of maturation-promoting factor from Xenopus.
1990,
Pubmed
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Xenbase
Gautier,
Purified maturation-promoting factor contains the product of a Xenopus homolog of the fission yeast cell cycle control gene cdc2+.
1988,
Pubmed
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Xenbase
Gautier,
Dephosphorylation and activation of Xenopus p34cdc2 protein kinase during the cell cycle.
1989,
Pubmed
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Xenbase
Gould,
Tyrosine phosphorylation of the fission yeast cdc2+ protein kinase regulates entry into mitosis.
1989,
Pubmed
Hayles,
The fission yeast cell cycle control gene cdc2: isolation of a sequence suc1 that suppresses cdc2 mutant function.
1986,
Pubmed
Kishimoto,
Generality of the action of various maturation-promoting factors.
1982,
Pubmed
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Xenbase
Labbe,
Purification of MPF from starfish: identification as the H1 histone kinase p34cdc2 and a possible mechanism for its periodic activation.
1989,
Pubmed
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Xenbase
Labbé,
MPF from starfish oocytes at first meiotic metaphase is a heterodimer containing one molecule of cdc2 and one molecule of cyclin B.
1989,
Pubmed
Langan,
Mammalian growth-associated H1 histone kinase: a homolog of cdc2+/CDC28 protein kinases controlling mitotic entry in yeast and frog cells.
1989,
Pubmed
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Xenbase
Lee,
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Pubmed
Lee,
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1988,
Pubmed
Lohka,
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Xenbase
Lohka,
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Minshull,
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1989,
Pubmed
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Xenbase
Morla,
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1989,
Pubmed
Murray,
The role of cyclin synthesis and degradation in the control of maturation promoting factor activity.
1989,
Pubmed
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Xenbase
Murray,
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1989,
Pubmed
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Xenbase
NULL,
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1988,
Pubmed
Pines,
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1987,
Pubmed
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Xenbase
Pines,
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1989,
Pubmed
Russell,
The mitotic inducer nim1+ functions in a regulatory network of protein kinase homologs controlling the initiation of mitosis.
1987,
Pubmed
Russell,
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1987,
Pubmed
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Pubmed
