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XB-ART-31961
Cell 1978 Jul 01;143:713-24.
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Sequence organization of a cloned tDNA met fragment from Xenopus laevis.

Clarkson SG , Kurer V , Smith HO .


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3.18 kb fragments of X. laevis DNA coding for tRNA 1 met have been inserted into a lambda vector via Hind III termini and cloned in E. coli. The organization of one cloned fragment has been analyzed by restriction endonuclease digestion and RNA-DNA hybridization. From the distribution of sites for three enzymes, this fragment appears to be typical of the majority of X. laevis tandem tDNA 1 met repeat units. Evidence is presented to suggest that it contains two genes coding for tRNA 1 met and at least one gene coding for a second as yet unidentified 4S RNA species. The two tRNA 1 met genes are located on the same DNA strand 0.96 and 1.38 kb from one end of the repeat unit. A detailed restriction map for 19 enzymes reveals that the spacers between these genes are not identical, and it provides no indication of short repetitive sequence elements within the spacers.

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Genes referenced: mt-tr trna