Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Functional modification of a 21-kilodalton G protein when ADP-ribosylated by exoenzyme C3 of Clostridium botulinum.
Rubin EJ
,
Gill DM
,
Boquet P
,
Popoff MR
.
???displayArticle.abstract???
Exoenzyme C3 from Clostridium botulinum types C and D specifically ADP-ribosylated a 21-kilodalton cellular protein, p21.bot. Guanyl nucleotides protected the substrate against denaturation, which implies that p21.bot is a G protein. When introduced into the interior of cells, purified exoenzyme C3 ADP-ribosylated intracellular p21.bot and changed its function. NIH 3T3, PC12, and other cells rapidly underwent temporary morphological alterations that were in certain respects similar to those seen after microinjection of cloned ras proteins. When injected into Xenopus oocytes, C3 induced migration of germinal vesicles and potentiated the cholera toxin-sensitive augmentation of germinal vesicle breakdown by progesterone, also as caused by ras proteins. Nevertheless, p21.bot was immunologically distinct from p21ras.
Aktories,
Clostridium botulinum type C produces a novel ADP-ribosyltransferase distinct from botulinum C2 toxin.
1987, Pubmed
Aktories,
Clostridium botulinum type C produces a novel ADP-ribosyltransferase distinct from botulinum C2 toxin.
1987,
Pubmed
Aktories,
Botulinum C2 toxin ADP-ribosylates actin.
,
Pubmed
Bar-Sagi,
Microinjection of the ras oncogene protein into PC12 cells induces morphological differentiation.
1985,
Pubmed
Birchmeier,
ras proteins can induce meiosis in Xenopus oocytes.
1985,
Pubmed
,
Xenbase
Burnette,
"Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.
1981,
Pubmed
Chardin,
The ral gene: a new ras related gene isolated by the use of a synthetic probe.
1986,
Pubmed
Cleveland,
Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
1977,
Pubmed
Eklund,
Bacteriophage and the toxigenicity of Clostridium botulinum type C.
1971,
Pubmed
Evans,
Purification of the major GTP-binding proteins from human placental membranes.
1986,
Pubmed
Feramisco,
Microinjection of the oncogene form of the human H-ras (T-24) protein results in rapid proliferation of quiescent cells.
1984,
Pubmed
Fodge,
Glucose utilization, pH reduction and density dependent inhibition in cultures of chick embryo fibroblasts.
1975,
Pubmed
Furth,
Monoclonal antibodies to the p21 products of the transforming gene of Harvey murine sarcoma virus and of the cellular ras gene family.
1982,
Pubmed
Gibbs,
Intrinsic GTPase activity distinguishes normal and oncogenic ras p21 molecules.
1984,
Pubmed
Gill,
Structure-activity relationships in diphtheria toxin.
1971,
Pubmed
Gill,
A second guanyl nucleotide-binding site associated with adenylate cyclase. Distinct nucleotides activate adenylate cyclase and permit ADP-ribosylation by cholera toxin.
1983,
Pubmed
Gilman,
G proteins and dual control of adenylate cyclase.
1984,
Pubmed
Hattori,
Reactivity of a sulfhydryl group of the ras oncogene product p21 modulated by GTP binding.
1986,
Pubmed
Kahn,
The protein cofactor necessary for ADP-ribosylation of Gs by cholera toxin is itself a GTP binding protein.
1986,
Pubmed
Lowe,
Structure of the human and murine R-ras genes, novel genes closely related to ras proto-oncogenes.
1987,
Pubmed
Madaule,
A novel ras-related gene family.
1985,
Pubmed
Miyazaki,
Clostridium botulinum type D toxin: purification, molecular structure, and some immunological properties.
1977,
Pubmed
Oguma,
Biochemical classification of Clostridium botulinum type C and D strains and their nontoxigenic derivatives.
1986,
Pubmed
Ohashi,
ADP-ribosylation of a Mr 21,000 membrane protein by type D botulinum toxin.
1987,
Pubmed
Ohishi,
Purification and characterization of two components of botulinum C2 toxin.
1980,
Pubmed
Oka,
Snake venom phosphodiesterase: simple purification with Blue Sepharose and its application to poly(ADP-ribose) study.
1978,
Pubmed
Okada,
Introduction of macromolecules into cultured mammalian cells by osmotic lysis of pinocytic vesicles.
1982,
Pubmed
Roa,
Interaction of tetanus toxin with lipid vesicles at low pH. Protection of specific polypeptides against proteolysis.
1985,
Pubmed
Schmitt,
The ras-related YPT1 gene product in yeast: a GTP-binding protein that might be involved in microtubule organization.
1986,
Pubmed
Shih,
Identification of a precursor in the biosynthesis of the p21 transforming protein of harvey murine sarcoma virus.
1982,
Pubmed
Sigal,
Identification of effector residues and a neutralizing epitope of Ha-ras-encoded p21.
1986,
Pubmed
Simpson,
Molecular pharmacology of botulinum toxin and tetanus toxin.
1986,
Pubmed
Stacey,
Transformation of NIH 3T3 cells by microinjection of Ha-ras p21 protein.
,
Pubmed
Tamir,
Transient states of adenylate cyclase in brain membranes.
1985,
Pubmed
