Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
The C-terminal domain of TAP interacts with the nuclear pore complex and promotes export of specific CTE-bearing RNA substrates.
Bachi A
,
Braun IC
,
Rodrigues JP
,
Panté N
,
Ribbeck K
,
von Kobbe C
,
Kutay U
,
Wilm M
,
Görlich D
,
Carmo-Fonseca M
,
Izaurralde E
.
???displayArticle.abstract???
Messenger RNAs are exported from the nucleus as large ribonucleoprotein complexes (mRNPs). To date, proteins implicated in this process include TAP/Mex67p and RAE1/Gle2p and are distinct from the nuclear transport receptors of the beta-related, Ran-binding protein family. Mex67p is essential for mRNA export in yeast. Its vertebrate homolog TAP has been implicated in the export of cellular mRNAs and of simian type D viral RNAs bearing the constitutive transport element (CTE). Here we show that TAP is predominantly localized in the nucleoplasm and at both the nucleoplasmic and cytoplasmic faces of the nuclear pore complex (NPC). TAP interacts with multiple components of the NPC including the nucleoporins CAN, Nup98, Nup153, p62, and with three major NPC subcomplexes. The nucleoporin-binding domain of TAP comprises residues 508-619. In HeLa cells, this domain is necessary and sufficient to target GFP-TAP fusions to the nuclear rim. Moreover, the isolated domain strongly competes multiple export pathways in vivo, probably by blocking binding sites on the NPC that are shared with other transport receptors. Microinjection experiments implicate this domain in the export of specific CTE-containing RNAs. Finally, we show that TAP interacts with transportin and with two proteins implicated in the export of cellular mRNAs: RAE1/hGle2 and E1B-AP5. The interaction of TAP with nucleoporins, its direct binding to the CTE RNA, and its association with two mRNP binding proteins suggest that TAP is an RNA export mediator that may bridge the interaction between specific RNP export substrates and the NPC.
Almeida,
Microinjection of anti-coilin antibodies affects the structure of coiled bodies.
1998, Pubmed
Almeida,
Microinjection of anti-coilin antibodies affects the structure of coiled bodies.
1998,
Pubmed
Askjaer,
RanGTP-regulated interactions of CRM1 with nucleoporins and a shuttling DEAD-box helicase.
1999,
Pubmed
,
Xenbase
Bailer,
Nup116p and nup100p are interchangeable through a conserved motif which constitutes a docking site for the mRNA transport factor gle2p.
1998,
Pubmed
,
Xenbase
Bear,
Identification of novel import and export signals of human TAP, the protein that binds to the constitutive transport element of the type D retrovirus mRNAs.
1999,
Pubmed
,
Xenbase
Bharathi,
The human RAE1 gene is a functional homologue of Schizosaccharomyces pombe rae1 gene involved in nuclear export of Poly(A)+ RNA.
1997,
Pubmed
Bischoff,
Co-activation of RanGTPase and inhibition of GTP dissociation by Ran-GTP binding protein RanBP1.
1995,
Pubmed
Bischoff,
Human RanGTPase-activating protein RanGAP1 is a homologue of yeast Rna1p involved in mRNA processing and transport.
1995,
Pubmed
Boelens,
Nuclear retention of RNA as a mechanism for localization.
1995,
Pubmed
,
Xenbase
Bogerd,
Inhibition of human immunodeficiency virus Rev and human T-cell leukemia virus Rex function, but not Mason-Pfizer monkey virus constitutive transport element activity, by a mutant human nucleoporin targeted to Crm1.
1998,
Pubmed
Braun,
TAP binds to the constitutive transport element (CTE) through a novel RNA-binding motif that is sufficient to promote CTE-dependent RNA export from the nucleus.
1999,
Pubmed
,
Xenbase
Brown,
A mutation in the Schizosaccharomyces pombe rae1 gene causes defects in poly(A)+ RNA export and in the cytoskeleton.
1995,
Pubmed
Dahlberg,
Functions of the GTPase Ran in RNA export from the nucleus.
1998,
Pubmed
Dignam,
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
1983,
Pubmed
Doye,
From nucleoporins to nuclear pore complexes.
1997,
Pubmed
Fischer,
The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs.
1995,
Pubmed
Fornerod,
The human homologue of yeast CRM1 is in a dynamic subcomplex with CAN/Nup214 and a novel nuclear pore component Nup88.
1997,
Pubmed
Fornerod,
CRM1 is an export receptor for leucine-rich nuclear export signals.
1997,
Pubmed
,
Xenbase
Fukuda,
CRM1 is responsible for intracellular transport mediated by the nuclear export signal.
1997,
Pubmed
,
Xenbase
Gabler,
E1B 55-kilodalton-associated protein: a cellular protein with RNA-binding activity implicated in nucleocytoplasmic transport of adenovirus and cellular mRNAs.
1998,
Pubmed
Görlich,
A novel class of RanGTP binding proteins.
1997,
Pubmed
,
Xenbase
Görlich,
Identification of different roles for RanGDP and RanGTP in nuclear protein import.
1996,
Pubmed
,
Xenbase
Grandi,
Nup93, a vertebrate homologue of yeast Nic96p, forms a complex with a novel 205-kDa protein and is required for correct nuclear pore assembly.
1997,
Pubmed
,
Xenbase
Grüter,
TAP, the human homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus.
1998,
Pubmed
,
Xenbase
Izaurralde,
The asymmetric distribution of the constituents of the Ran system is essential for transport into and out of the nucleus.
1997,
Pubmed
Kang,
The human Tap protein is a nuclear mRNA export factor that contains novel RNA-binding and nucleocytoplasmic transport sequences.
1999,
Pubmed
Katahira,
The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human.
1999,
Pubmed
Kehlenbach,
A role for RanBP1 in the release of CRM1 from the nuclear pore complex in a terminal step of nuclear export.
1999,
Pubmed
Klebe,
Interaction of the nuclear GTP-binding protein Ran with its regulatory proteins RCC1 and RanGAP1.
1995,
Pubmed
,
Xenbase
Kraemer,
mRNA binding protein mrnp 41 localizes to both nucleus and cytoplasm.
1997,
Pubmed
Kutay,
Dominant-negative mutants of importin-beta block multiple pathways of import and export through the nuclear pore complex.
1997,
Pubmed
,
Xenbase
Mahajan,
A small ubiquitin-related polypeptide involved in targeting RanGAP1 to nuclear pore complex protein RanBP2.
1997,
Pubmed
Mann,
Error-tolerant identification of peptides in sequence databases by peptide sequence tags.
1994,
Pubmed
Mattaj,
Nucleocytoplasmic transport: the soluble phase.
1998,
Pubmed
Matunis,
A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex.
1996,
Pubmed
,
Xenbase
Murphy,
GLE2, a Saccharomyces cerevisiae homologue of the Schizosaccharomyces pombe export factor RAE1, is required for nuclear pore complex structure and function.
1996,
Pubmed
Nakielny,
Nuclear export of proteins and RNAs.
1997,
Pubmed
Nakielny,
Nup153 is an M9-containing mobile nucleoporin with a novel Ran-binding domain.
1999,
Pubmed
Neville,
The importin-beta family member Crm1p bridges the interaction between Rev and the nuclear pore complex during nuclear export.
1997,
Pubmed
Otero,
Leptomycin B inhibits equine infectious anemia virus Rev and feline immunodeficiency virus rev function but not the function of the hepatitis B virus posttranscriptional regulatory element.
1998,
Pubmed
Panté,
Interactions and three-dimensional localization of a group of nuclear pore complex proteins.
1994,
Pubmed
,
Xenbase
Paraskeva,
CRM1-mediated recycling of snurportin 1 to the cytoplasm.
1999,
Pubmed
,
Xenbase
Pasquinelli,
The constitutive transport element (CTE) of Mason-Pfizer monkey virus (MPMV) accesses a cellular mRNA export pathway.
1997,
Pubmed
,
Xenbase
Pollard,
A novel receptor-mediated nuclear protein import pathway.
1996,
Pubmed
Pritchard,
RAE1 is a shuttling mRNA export factor that binds to a GLEBS-like NUP98 motif at the nuclear pore complex through multiple domains.
1999,
Pubmed
,
Xenbase
Radu,
The peptide repeat domain of nucleoporin Nup98 functions as a docking site in transport across the nuclear pore complex.
1995,
Pubmed
Rexach,
Protein import into nuclei: association and dissociation reactions involving transport substrate, transport factors, and nucleoporins.
1995,
Pubmed
Richards,
Requirement of guanosine triphosphate-bound ran for signal-mediated nuclear protein export.
1997,
Pubmed
Saavedra,
The simian retrovirus-1 constitutive transport element, unlike the HIV-1 RRE, uses factors required for cellular mRNA export.
1997,
Pubmed
,
Xenbase
Saitoh,
RanBP2 associates with Ubc9p and a modified form of RanGAP1.
1997,
Pubmed
,
Xenbase
Santos-Rosa,
Nuclear mRNA export requires complex formation between Mex67p and Mtr2p at the nuclear pores.
1998,
Pubmed
Schmitt,
Dbp5, a DEAD-box protein required for mRNA export, is recruited to the cytoplasmic fibrils of nuclear pore complex via a conserved interaction with CAN/Nup159p.
1999,
Pubmed
,
Xenbase
Segref,
Mex67p, a novel factor for nuclear mRNA export, binds to both poly(A)+ RNA and nuclear pores.
1997,
Pubmed
Shevchenko,
Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels.
1996,
Pubmed
Siomi,
A nuclear localization domain in the hnRNP A1 protein.
1995,
Pubmed
Siomi,
Transportin-mediated nuclear import of heterogeneous nuclear RNP proteins.
1997,
Pubmed
Snay-Hodge,
Dbp5p/Rat8p is a yeast nuclear pore-associated DEAD-box protein essential for RNA export.
1998,
Pubmed
Stade,
Exportin 1 (Crm1p) is an essential nuclear export factor.
1997,
Pubmed
Tseng,
Dbp5p, a cytosolic RNA helicase, is required for poly(A)+ RNA export.
1998,
Pubmed
,
Xenbase
Vankan,
Roles of U4 and U6 snRNAs in the assembly of splicing complexes.
1992,
Pubmed
,
Xenbase
Wilm,
Femtomole sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry.
1996,
Pubmed
Wu,
Nup358, a cytoplasmically exposed nucleoporin with peptide repeats, Ran-GTP binding sites, zinc fingers, a cyclophilin A homologous domain, and a leucine-rich region.
1995,
Pubmed
Yokoyama,
A giant nucleopore protein that binds Ran/TC4.
1995,
Pubmed
Zolotukhin,
Mutations in the nuclear export signal of human ran-binding protein RanBP1 block the Rev-mediated posttranscriptional regulation of human immunodeficiency virus type 1.
1997,
Pubmed
Zolotukhin,
Nucleoporins nup98 and nup214 participate in nuclear export of human immunodeficiency virus type 1 Rev.
1999,
Pubmed
,
Xenbase
