Xenbase may experience sporadic downtime from September 1st to September 3rd due to scheduled IT maintenance work.
We apologize for the inconvenience.
Click on this message to dismiss it.
Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Reversal of rectification and alteration of selectivity and pharmacology in a mammalian Kv1.1 potassium channel by deletion of domains S1 to S4.
Tytgat J
,
Vereecke J
,
Carmeliet E
.
???displayArticle.abstract???
1. A possible relation between the family of inwardly rectifying K+ channels and the Shaker superfamily of K+ channels was investigated using a deletion mutant (DelS1-S4) of a delayed rectifier Kv1.1 (RCK1) K+ channel. 2. The mutant DelS1-S4 was made by eliminating the sequence coding for transmembrane domains S1 to S4 of the Kv1.1 K+ channel, and re-ligating the sequence coding for the cytoplasmic amino terminus to transmembrane domain S5. Microelectrode voltage-clamp and patch-clamp experiments were performed on Xenopus laevis oocytes after injection of in vitro transcribed mRNA coding for mutant and wild-type channels. 3. The lack of transmembrane domains S1 to S4 converts a depolarization-activated wild-type Kv1.1 K+ channel with outward rectification into a hyperpolarization-activated channel with inward rectification. Although the pore region of the deletion mutant is identical to the wild-type channel, the mutant channel is a non-selective cation channel and is characterized by an altered pharmacology profile.
Babila,
Assembly of mammalian voltage-gated potassium channels: evidence for an important role of the first transmembrane segment.
1994, Pubmed,
Xenbase
Babila,
Assembly of mammalian voltage-gated potassium channels: evidence for an important role of the first transmembrane segment.
1994,
Pubmed
,
Xenbase
Christie,
Expression of a cloned rat brain potassium channel in Xenopus oocytes.
1989,
Pubmed
,
Xenbase
Ho,
Cloning and expression of an inwardly rectifying ATP-regulated potassium channel.
1993,
Pubmed
,
Xenbase
Hurst,
Identification of amino acid residues involved in dendrotoxin block of rat voltage-dependent potassium channels.
1991,
Pubmed
,
Xenbase
Inoue,
Activation-inactivation of potassium channels and development of the potassium-channel spike in internally perfused squid giant axons.
1981,
Pubmed
Kowdley,
Hyperpolarization-activated chloride currents in Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Kubo,
Primary structure and functional expression of a rat G-protein-coupled muscarinic potassium channel.
1993,
Pubmed
,
Xenbase
Kubo,
Primary structure and functional expression of a mouse inward rectifier potassium channel.
1993,
Pubmed
,
Xenbase
Lewis,
Ion-concentration dependence of the reversal potential and the single channel conductance of ion channels at the frog neuromuscular junction.
1979,
Pubmed
Li,
Specification of subunit assembly by the hydrophilic amino-terminal domain of the Shaker potassium channel.
1992,
Pubmed
Liman,
Subunit stoichiometry of a mammalian K+ channel determined by construction of multimeric cDNAs.
1992,
Pubmed
,
Xenbase
MacKinnon,
Determination of the subunit stoichiometry of a voltage-activated potassium channel.
1991,
Pubmed
,
Xenbase
Nichols,
The 'inner core' of inwardly rectifying K+ channels.
1993,
Pubmed
Nilius,
Permeation properties of a non-selective cation channel in human vascular endothelial cells.
1990,
Pubmed
Schachtman,
Expression of an inward-rectifying potassium channel by the Arabidopsis KAT1 cDNA.
1992,
Pubmed
,
Xenbase
Schroeder,
K+ transport properties of K+ channels in the plasma membrane of Vicia faba guard cells.
1988,
Pubmed
Shen,
Deletion analysis of K+ channel assembly.
1993,
Pubmed
,
Xenbase
Slesinger,
The S4-S5 loop contributes to the ion-selective pore of potassium channels.
1993,
Pubmed
,
Xenbase
Stühmer,
Potassium channels expressed from rat brain cDNA have delayed rectifier properties.
1988,
Pubmed
,
Xenbase
