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EMBO J
1993 Feb 01;122:513-25. doi: 10.1002/j.1460-2075.1993.tb05683.x.
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Recognition of the Xenopus ribosomal core promoter by the transcription factor xUBF involves multiple HMG box domains and leads to an xUBF interdomain interaction.
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The interaction of the ribosomal transcription factor xUBF with the RNA polymerase I core promoter of Xenopus laevis has been studied both at the DNA and protein levels. It is shown that a single xUBF-DNA complex forms over the 40S initiation site (+1) and involves at least the DNA sequences between -20 and +60 bp. DNA sequences upstream of +10 and downstream of +18 are each sufficient to direct complex formation independently. HMG box 1 of xUBF independently recognizes the sequences -20 to -1 and +1 to +22 and the addition of the N-terminal dimerization domain to HMG box 1 stabilizes its interaction with these sequences approximately 10-fold. HMG boxes 2/3 interact with the DNA downstream of +22 and can independently position xUBF across the initiation site. The C-terminal segment of xUBF, HMG boxes 4, 5 or the acidic domain, directly or indirectly interact with HMG box 1, making the core promoter sequences between -11 and -15 hypersensitive to DNase. This interaction also requires the DNA sequences between +17 and +32, i.e. the HMG box 2/3 binding site. The data suggest extensive folding of the core promoter within the xUBF complex.
Bachvarov,
The RNA polymerase I transcription factor xUBF contains 5 tandemly repeated HMG homology boxes.
1991, Pubmed,
Xenbase
Bachvarov,
The RNA polymerase I transcription factor xUBF contains 5 tandemly repeated HMG homology boxes.
1991,
Pubmed
,
Xenbase
Bachvarov,
Heterogeneity in the Xenopus ribosomal transcription factor xUBF has a molecular basis distinct from that in mammals.
1991,
Pubmed
,
Xenbase
Bateman,
Regulation of eukaryotic ribosomal RNA transcription by RNA polymerase modification.
1986,
Pubmed
Bell,
Functional cooperativity between transcription factors UBF1 and SL1 mediates human ribosomal RNA synthesis.
1988,
Pubmed
Bell,
Molecular mechanisms governing species-specific transcription of ribosomal RNA.
1989,
Pubmed
,
Xenbase
Cary,
Conformation and domain structure of the non-histone chromosomal proteins, HMG 1 and 2. Isolation of two folded fragments from HMG 1 and 2.
1983,
Pubmed
Comai,
The TATA-binding protein and associated factors are integral components of the RNA polymerase I transcription factor, SL1.
1992,
Pubmed
Cormack,
The TATA-binding protein is required for transcription by all three nuclear RNA polymerases in yeast cells.
1992,
Pubmed
Devereux,
A comprehensive set of sequence analysis programs for the VAX.
1984,
Pubmed
Diffley,
A close relative of the nuclear, chromosomal high-mobility group protein HMG1 in yeast mitochondria.
1991,
Pubmed
Firek,
Point mutation analysis of the Xenopus laevis RNA polymerase I core promoter.
1990,
Pubmed
,
Xenbase
Gething,
Protein folding in the cell.
1992,
Pubmed
Giese,
DNA-binding properties of the HMG domain of the lymphoid-specific transcriptional regulator LEF-1.
1991,
Pubmed
Giese,
The HMG domain of lymphoid enhancer factor 1 bends DNA and facilitates assembly of functional nucleoprotein structures.
1992,
Pubmed
Gokal,
Hormonal regulation of transcription of rDNA. Formation of initiated complexes by RNA polymerase I in vitro.
1990,
Pubmed
Gubbay,
A gene mapping to the sex-determining region of the mouse Y chromosome is a member of a novel family of embryonically expressed genes.
1990,
Pubmed
Guimond,
Variants of the Xenopus laevis ribosomal transcription factor xUBF are developmentally regulated by differential splicing.
1992,
Pubmed
,
Xenbase
Harley,
DNA binding activity of recombinant SRY from normal males and XY females.
1992,
Pubmed
Hewish,
Chromatin sub-structure. The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonuclease.
1973,
Pubmed
Hisatake,
Cloning and structural analysis of cDNA and the gene for mouse transcription factor UBF.
1991,
Pubmed
Jantzen,
Nucleolar transcription factor hUBF contains a DNA-binding motif with homology to HMG proteins.
1990,
Pubmed
Kolodrubetz,
Duplicated NHP6 genes of Saccharomyces cerevisiae encode proteins homologous to bovine high mobility group protein 1.
1990,
Pubmed
Kuipers,
Improved site-directed mutagenesis method using PCR.
1991,
Pubmed
Landsman,
Assessment of the transcriptional activation potential of the HMG chromosomal proteins.
1991,
Pubmed
Landt,
A general method for rapid site-directed mutagenesis using the polymerase chain reaction.
1990,
Pubmed
Lilley,
DNA--protein interactions. HMG has DNA wrapped up.
1992,
Pubmed
Mahajan,
Hormonal regulation of transcription of rDNA. The role of TFIC in formation of initiation complexes.
1990,
Pubmed
Mahajan,
Hormonal regulation of transcription of rDNA. Purification and characterization of the hormone-regulated transcription factor IC.
1990,
Pubmed
McStay,
xUBF and Rib 1 are both required for formation of a stable polymerase I promoter complex in X. laevis.
1991,
Pubmed
,
Xenbase
McStay,
xUBF contains a novel dimerization domain essential for RNA polymerase I transcription.
1991,
Pubmed
,
Xenbase
Miller,
Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes.
1985,
Pubmed
,
Xenbase
Moss,
More ribosomal spacer sequences from Xenopus laevis.
1980,
Pubmed
,
Xenbase
Moss,
Transcription of cloned Xenopus laevis ribosomal DNA microinjected into Xenopus oocytes, and the identification of an RNA polymerase I promoter.
1982,
Pubmed
,
Xenbase
Moss,
The promotion of ribosomal transcription in eukaryotes.
1985,
Pubmed
Nasrin,
DNA-binding properties of the product of the testis-determining gene and a related protein.
1991,
Pubmed
O'Mahony,
Analysis of the phosphorylation, DNA-binding and dimerization properties of the RNA polymerase I transcription factors UBF1 and UBF2.
1992,
Pubmed
Parisi,
Similarity of human mitochondrial transcription factor 1 to high mobility group proteins.
1991,
Pubmed
Paule,
Initiation and regulation mechanisms of ribosomal RNA transcription in the eukaryote Acanthamoeba castellanii.
,
Pubmed
Pikaard,
The Xenopus ribosomal gene enhancers bind an essential polymerase I transcription factor, xUBF.
1989,
Pubmed
,
Xenbase
Ranish,
Isolation of two genes that encode subunits of the yeast transcription factor IIA.
1992,
Pubmed
Read,
High resolution studies of the Xenopus laevis ribosomal gene promoter in vivo and in vitro.
1992,
Pubmed
,
Xenbase
Reeck,
Domain structure in high molecular weight high mobility group nonhistone chromatin proteins.
1982,
Pubmed
Reeves,
The A.T-DNA-binding domain of mammalian high mobility group I chromosomal proteins. A novel peptide motif for recognizing DNA structure.
1990,
Pubmed
Saluz,
A simple high-resolution procedure to study DNA methylation and in vivo DNA-protein interactions on a single-copy gene level in higher eukaryotes.
1989,
Pubmed
Schnapp,
A growth-dependent transcription initiation factor (TIF-IA) interacting with RNA polymerase I regulates mouse ribosomal RNA synthesis.
1990,
Pubmed
Schnapp,
Isolation and functional characterization of TIF-IB, a factor that confers promoter specificity to mouse RNA polymerase I.
1990,
Pubmed
Schultz,
Variants of the TATA-binding protein can distinguish subsets of RNA polymerase I, II, and III promoters.
1992,
Pubmed
Sinclair,
A gene from the human sex-determining region encodes a protein with homology to a conserved DNA-binding motif.
1990,
Pubmed
Smith,
Characterization of factors that direct transcription of rat ribosomal DNA.
1990,
Pubmed
Sollner-Webb,
News from the nucleolus: rRNA gene expression.
1991,
Pubmed
Stewart,
Multiple heterogeneities in the transcribed spacers of ribosomal DNA from Xenopus laevis.
1983,
Pubmed
,
Xenbase
Suzuki,
The heptad repeat in the largest subunit of RNA polymerase II binds by intercalating into DNA.
1990,
Pubmed
Suzuki,
SPKK, a new nucleic acid-binding unit of protein found in histone.
1989,
Pubmed
Tanaka,
Sequence-specific binding of a transcription factor TFID to the promoter region of mouse ribosomal RNA gene.
1990,
Pubmed
Tower,
Transcription of mouse rDNA is regulated by an activated subform of RNA polymerase I.
1987,
Pubmed
Travis,
LEF-1, a gene encoding a lymphoid-specific protein with an HMG domain, regulates T-cell receptor alpha enhancer function [corrected].
1991,
Pubmed
van de Wetering,
Identification and cloning of TCF-1, a T lymphocyte-specific transcription factor containing a sequence-specific HMG box.
1991,
Pubmed
Voit,
The nucleolar transcription factor mUBF is phosphorylated by casein kinase II in the C-terminal hyperacidic tail which is essential for transactivation.
1992,
Pubmed
White,
A role for the TATA-box-binding protein component of the transcription factor IID complex as a general RNA polymerase III transcription factor.
1992,
Pubmed
