XB-ART-22013
Mol Cell Biol
1993 Nov 01;1311:6819-31. doi: 10.1128/mcb.13.11.6819-6831.1993.
Show Gene links
Show Anatomy links
Structural requirements of 5S rRNA for nuclear transport, 7S ribonucleoprotein particle assembly, and 60S ribosomal subunit assembly in Xenopus oocytes.
Allison LA
,
North MT
,
Murdoch KJ
,
Romaniuk PJ
,
Deschamps S
,
le Maire M
.
???displayArticle.abstract???
Structural requirements of 5S rRNA for nuclear transport and RNA-protein interactions have been studied by analyzing the behavior of oocyte-type 5S rRNA and of 31 different in vitro-generated mutant transcripts after microinjection into the cytoplasm of Xenopus oocytes. Experiments reveal that the sequence and secondary and/or tertiary structure requirements of 5S rRNA for nuclear transport, storage in the cytoplasm as 7S ribonucleoprotein particles, and assembly into 60S ribosomal subunits are complex and nonidentical. Elements of loops A, C, and E, helices II and V, and bulged and hinge nucleotides in the central domain of 5S rRNA carry the essential information for these functional activities. Assembly of microinjected 5S rRNA into 60S ribosomal subunits was shown to occur in the nucleus; thus, the first requirement for subunit assembly is nuclear targeting. The inhibitory effects of ATP depletion, wheat germ agglutinin, and chilling on the nuclear import of 5S rRNA indicate that it crosses the nuclear envelope through the nuclear pore complex by a pathway similar to that used by karyophilic proteins.
???displayArticle.pubmedLink??? 8413275
???displayArticle.pmcLink??? PMC364744
???displayArticle.link??? Mol Cell Biol
Species referenced: Xenopus laevis
Genes referenced: nup62
???displayArticle.antibodies??? Nup62 Ab2
References [+] :
Adam,
Cytosolic proteins that specifically bind nuclear location signals are receptors for nuclear import.
1991, Pubmed
Adam, Cytosolic proteins that specifically bind nuclear location signals are receptors for nuclear import. 1991, Pubmed
Akey, Protein import through the nuclear pore complex is a multistep process. 1989, Pubmed , Xenbase
Allison, RNA-protein interactions of stored 5S RNA with TFIIIA and ribosomal protein L5 during Xenopus oogenesis. 1991, Pubmed , Xenbase
Bartel, HIV-1 Rev regulation involves recognition of non-Watson-Crick base pairs in viral RNA. 1991, Pubmed
Baserga, Distinct molecular signals for nuclear import of the nucleolar snRNA, U3. 1992, Pubmed , Xenbase
Bataillé, Cytoplasmic transport of ribosomal subunits microinjected into the Xenopus laevis oocyte nucleus: a generalized, facilitated process. 1990, Pubmed , Xenbase
Baudin, A difference in the importance of bulged nucleotides and their parent base pairs in the binding of transcription factor IIIA to Xenopus 5S RNA and 5S RNA genes. 1989, Pubmed , Xenbase
Baudin, Involvement of "hinge" nucleotides of Xenopus laevis 5 S rRNA in the RNA structural organization and in the binding of transcription factor TFIIIA. 1991, Pubmed , Xenbase
Borer, Major nucleolar proteins shuttle between nucleus and cytoplasm. 1989, Pubmed , Xenbase
Breeuwer, Facilitated nuclear transport of histone H1 and other small nucleophilic proteins. 1990, Pubmed
Brow, Modulation of yeast 5 S rRNA synthesis in vitro by ribosomal protein YL3. A possible regulatory loop. 1987, Pubmed
Brunel, Effect of mutations in domain 2 on the structural organization of oocyte 5 S rRNA from Xenopus laevis. 1990, Pubmed , Xenbase
Chow, Delineation of structural domains in eukaryotic 5S rRNA with a rhodium probe. 1992, Pubmed , Xenbase
Cullen, The HIV-1 Tat protein: an RNA sequence-specific processivity factor? 1990, Pubmed
Dabauvalle, Inhibition of nuclear accumulation of karyophilic proteins in living cells by microinjection of the lectin wheat germ agglutinin. 1988, Pubmed , Xenbase
Dargemont, Export of mRNA from microinjected nuclei of Xenopus laevis oocytes. 1992, Pubmed , Xenbase
Denis, Thesaurisomes, a novel kind of nucleoprotein particle. 1983, Pubmed , Xenbase
De Robertis, Intracellular transport of microinjected 5S and small nuclear RNAs. 1982, Pubmed , Xenbase
de Stevenson, Structural studies on site-directed mutants of domain 3 of Xenopus laevis oocyte 5 S ribosomal RNA. 1991, Pubmed , Xenbase
Draper, How do proteins recognize specific RNA sites? New clues from autogenously regulated ribosomal proteins. 1989, Pubmed
Dworetzky, The effects of variations in the number and sequence of targeting signals on nuclear uptake. 1988, Pubmed , Xenbase
Dworetzky, Translocation of RNA-coated gold particles through the nuclear pores of oocytes. 1988, Pubmed , Xenbase
Eckner, Mature mRNA 3' end formation stimulates RNA export from the nucleus. 1991, Pubmed
Fabijanski, Identification of proteins at the peptidyl-tRNA binding site of rat liver ribosomes. 1981, Pubmed
Featherstone, A monoclonal antibody against the nuclear pore complex inhibits nucleocytoplasmic transport of protein and RNA in vivo. 1988, Pubmed , Xenbase
Finlay, A complex of nuclear pore proteins required for pore function. 1991, Pubmed
Finlay, Reconstitution of biochemically altered nuclear pores: transport can be eliminated and restored. 1990, Pubmed , Xenbase
Fischer, Diversity in the signals required for nuclear accumulation of U snRNPs and variety in the pathways of nuclear transport. 1991, Pubmed , Xenbase
Fischer, Nucleo-cytoplasmic transport of U snRNPs: definition of a nuclear location signal in the Sm core domain that binds a transport receptor independently of the m3G cap. 1993, Pubmed , Xenbase
Goldfarb, Pathways for the nuclear transport of proteins and RNAs. 1991, Pubmed
Guddat, Protein-mediated nuclear export of RNA: 5S rRNA containing small RNPs in xenopus oocytes. 1990, Pubmed , Xenbase
Hamm, The trimethylguanosine cap structure of U1 snRNA is a component of a bipartite nuclear targeting signal. 1990, Pubmed , Xenbase
Hamm, Monomethylated cap structures facilitate RNA export from the nucleus. 1990, Pubmed , Xenbase
Holmberg, Ribosome-bound eukaryotic elongation factor 2 protects 5 S rRNA from modification. 1992, Pubmed
Huber, Use of the cytotoxic nuclease alpha-sarcin to identify the binding site on eukaryotic 5 S ribosomal ribonucleic acid for the ribosomal protein L5. 1986, Pubmed , Xenbase
Imamoto, Antibodies against 70-kD heat shock cognate protein inhibit mediated nuclear import of karyophilic proteins. 1992, Pubmed
Kelly, The nucleotide sequence at the 3'-end of Neurospora crassa 18S-rRNA and studies on the interaction with 5S-rRNA. 1982, Pubmed
Khanna-Gupta, Nucleocytoplasmic transport of ribosomes in a eukaryotic system: is there a facilitated transport process? 1989, Pubmed , Xenbase
Lagaye, Characterization by human antibodies of two HeLa cell proteins which are related to Xenopus laevis transcription factor TFIIIA. 1988, Pubmed , Xenbase
le Maire, Biochemical research on oogenesis. Binding of tRNA to the nucleoprotein particles of Xenopus laevis previtellogenic oocytes. 1987, Pubmed , Xenbase
Lo, Topography of 5.8 S rRNA in rat liver ribosomes. Identification of diethyl pyrocarbonate-reactive sites. 1982, Pubmed
Martin, Nuclear transport of influenza virus ribonucleoproteins: the viral matrix protein (M1) promotes export and inhibits import. 1991, Pubmed
Mattaj, Nuclear exclusion of transcription factor IIIA and the 42s particle transfer RNA-binding protein in Xenopus oocytes: a possible mechanism for gene control? 1983, Pubmed , Xenbase
Mehlin, Translocation of a specific premessenger ribonucleoprotein particle through the nuclear pore studied with electron microscope tomography. 1992, Pubmed
Meier, Nopp140 shuttles on tracks between nucleolus and cytoplasm. 1992, Pubmed
Michaud, Multiple pathways in nuclear transport: the import of U2 snRNP occurs by a novel kinetic pathway. 1991, Pubmed , Xenbase
Michaud, Microinjected U snRNAs are imported to oocyte nuclei via the nuclear pore complex by three distinguishable targeting pathways. 1992, Pubmed , Xenbase
Moore, The two steps of nuclear import, targeting to the nuclear envelope and translocation through the nuclear pore, require different cytosolic factors. 1992, Pubmed , Xenbase
Neuman de Vegvar, Nucleocytoplasmic transport and processing of small nuclear RNA precursors. 1990, Pubmed , Xenbase
Newmeyer, Nuclear import can be separated into distinct steps in vitro: nuclear pore binding and translocation. 1988, Pubmed , Xenbase
Peters, Nucleo-cytoplasmic flux and intracellular mobility in single hepatocytes measured by fluorescence microphotolysis. 1984, Pubmed
Pierandrei-Amaldi, Messenger RNA for ribosomal proteins in Xenopus laevis oocytes. 1980, Pubmed , Xenbase
Pruijn, Analysis of protein--RNA interactions within Ro ribonucleoprotein complexes. 1991, Pubmed
Richardson, Nuclear protein migration involves two steps: rapid binding at the nuclear envelope followed by slower translocation through nuclear pores. 1988, Pubmed , Xenbase
Romaniuk, Defining the binding site of Xenopus transcription factor IIIA on 5S RNA using truncated and chimeric 5S RNA molecules. 1987, Pubmed , Xenbase
Romaniuk, A comparison of the solution structures and conformational properties of the somatic and oocyte 5S rRNAs of Xenopus laevis. 1988, Pubmed , Xenbase
Romaniuk, The role of highly conserved single-stranded nucleotides of Xenopus 5S RNA in the binding of transcription factor IIIA. 1989, Pubmed , Xenbase
Sarge, Intermolecular hybridization of 5S rRNA with 18S rRNA: identification of a 5'-terminally-located nucleotide sequence in mouse 5S rRNA which base-pairs with two specific complementary sequences in 18S rRNA. 1991, Pubmed
Shi, The transport of proteins into the nucleus requires the 70-kilodalton heat shock protein or its cytosolic cognate. 1992, Pubmed , Xenbase
Silver, How proteins enter the nucleus. 1991, Pubmed
Singh, Sequence-specific binding of transfer RNA by glyceraldehyde-3-phosphate dehydrogenase. 1993, Pubmed
Stebbins-Boaz, Structural analysis of the peptidyl transferase region in ribosomal RNA of the eukaryote Xenopus laevis. 1991, Pubmed , Xenbase
Steitz, A 5S rRNA/L5 complex is a precursor to ribosome assembly in mammalian cells. 1988, Pubmed
Sterne-Marr, O-linked glycoproteins of the nuclear pore complex interact with a cytosolic factor required for nuclear protein import. 1992, Pubmed
Theunissen, RNA and DNA binding zinc fingers in Xenopus TFIIIA. 1992, Pubmed , Xenbase
Tobian, tRNA nuclear transport: defining the critical regions of human tRNAimet by point mutagenesis. 1985, Pubmed , Xenbase
Uchiumi, Cross-linking study on protein neighborhoods at the subunit interface of rat liver ribosomes with 2-iminothiolane. 1986, Pubmed
Van Ryk, Unbalanced ribosome assembly in Saccharomyces cerevisiae expressing mutant 5 S rRNAs. 1992, Pubmed
Viel, Elongation factor 1 alpha (EF-1 alpha) is concentrated in the Balbiani body and accumulates coordinately with the ribosomes during oogenesis of Xenopus laevis. 1990, Pubmed , Xenbase
Westhof, Computer modeling from solution data of spinach chloroplast and of Xenopus laevis somatic and oocyte 5 S rRNAs. 1989, Pubmed , Xenbase
Wimberly, The conformation of loop E of eukaryotic 5S ribosomal RNA. 1993, Pubmed , Xenbase
Wormington, Developmental expression and 5S rRNA-binding activity of Xenopus laevis ribosomal protein L5. 1989, Pubmed , Xenbase
You, The effects of disrupting 5S RNA helical structures on the binding of Xenopus transcription factor IIIA. 1990, Pubmed , Xenbase
Zasloff, tRNA transport from the nucleus in a eukaryotic cell: carrier-mediated translocation process. 1983, Pubmed , Xenbase