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Multiple pathways in nuclear transport: the import of U2 snRNP occurs by a novel kinetic pathway.
Michaud N
,
Goldfarb DS
.
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Protein import to the nucleus is a signal-mediated process that exhibits saturation kinetics. We investigated whether signal bearing proteins compete with U2 and U6 snRNPs during import. When injected into Xenopus oocytes, saturating concentrations of P(Lys)-BSA, a protein bearing multiple nuclear localization signals from SV40 large T-antigen, reduce the rate of [125I]P(Lys)-BSA and of [125I]nucleoplasmin import, consistent with their competing for and sharing the same limiting component of the import apparatus. In contrast, saturating concentrations of P(Lys)-BSA do not reduce the rate of HeLa [32P]U2 snRNP assembly or import. The import of U6 snRNP is also competed by P(Lys)-BSA. We conclude that U2 snRNP is imported into oocyte nuclei by a kinetic pathway that is distinct from the one followed by P(Lys)-BSA, nucleoplasmin, and U6 snRNP.
Adam,
Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic factors.
1990, Pubmed,
Xenbase
Adam,
Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic factors.
1990,
Pubmed
,
Xenbase
Adam,
Identification of specific binding proteins for a nuclear location sequence.
1989,
Pubmed
Akey,
Protein import through the nuclear pore complex is a multistep process.
1989,
Pubmed
,
Xenbase
Akey,
Visualization of transport-related configurations of the nuclear pore transporter.
1990,
Pubmed
Bach,
20S small nuclear ribonucleoprotein U5 shows a surprisingly complex protein composition.
1989,
Pubmed
Bataillé,
Cytoplasmic transport of ribosomal subunits microinjected into the Xenopus laevis oocyte nucleus: a generalized, facilitated process.
1990,
Pubmed
,
Xenbase
Benditt,
Interaction of a nuclear location signal with isolated nuclear envelopes and identification of signal-binding proteins by photoaffinity labeling.
1989,
Pubmed
Bernstein,
Cytosolic protein translocation factors. Is SRP still unique?
1989,
Pubmed
Borer,
Major nucleolar proteins shuttle between nucleus and cytoplasm.
1989,
Pubmed
,
Xenbase
Breeuwer,
Facilitated nuclear transport of histone H1 and other small nucleophilic proteins.
1990,
Pubmed
Bürglin,
The nuclear migration signal of Xenopus laevis nucleoplasmin.
1987,
Pubmed
,
Xenbase
Chelsky,
Sequence requirements for synthetic peptide-mediated translocation to the nucleus.
1989,
Pubmed
,
Xenbase
De Robertis,
Intracellular transport of microinjected 5S and small nuclear RNAs.
1982,
Pubmed
,
Xenbase
Dingwall,
The nucleoplasmin nuclear location sequence is larger and more complex than that of SV-40 large T antigen.
1988,
Pubmed
Dingwall,
Protein import into the cell nucleus.
1986,
Pubmed
Dreyer,
Two-dimensional gel analysis of the fate of oocyte nuclear proteins in the development of Xenopus laevis.
1983,
Pubmed
,
Xenbase
Dworetzky,
The effects of variations in the number and sequence of targeting signals on nuclear uptake.
1988,
Pubmed
,
Xenbase
Finlay,
Nuclear transport in vitro.
1989,
Pubmed
,
Xenbase
Fischer,
An essential signaling role for the m3G cap in the transport of U1 snRNP to the nucleus.
1990,
Pubmed
,
Xenbase
Gerace,
Functional organization of the nuclear envelope.
1988,
Pubmed
Goldfarb,
Synthetic peptides as nuclear localization signals.
,
Pubmed
,
Xenbase
Goldfarb,
Nuclear transport.
1989,
Pubmed
Gusse,
Translocation of a store of maternal cytoplasmic c-myc protein into nuclei during early development.
1989,
Pubmed
,
Xenbase
Hamm,
An abundant U6 snRNP found in germ cells and embryos of Xenopus laevis.
1989,
Pubmed
,
Xenbase
Hamm,
The trimethylguanosine cap structure of U1 snRNA is a component of a bipartite nuclear targeting signal.
1990,
Pubmed
,
Xenbase
Hartl,
Mitochondrial protein import.
1989,
Pubmed
Hunt,
Cytoplasmic anchoring proteins and the control of nuclear localization.
1989,
Pubmed
Khanna-Gupta,
Nucleocytoplasmic transport of ribosomes in a eukaryotic system: is there a facilitated transport process?
1989,
Pubmed
,
Xenbase
Konarska,
Analysis of splicing complexes and small nuclear ribonucleoprotein particles by native gel electrophoresis.
1989,
Pubmed
Lanford,
Comparison of diverse transport signals in synthetic peptide-induced nuclear transport.
1990,
Pubmed
,
Xenbase
Lanford,
Effect of basic and nonbasic amino acid substitutions on transport induced by simian virus 40 T-antigen synthetic peptide nuclear transport signals.
1988,
Pubmed
Lanford,
Induction of nuclear transport with a synthetic peptide homologous to the SV40 T antigen transport signal.
1986,
Pubmed
Lee,
Identification and characterization of a nuclear localization sequence-binding protein in yeast.
1989,
Pubmed
Li,
Identification of a human protein that interacts with nuclear localization signals.
1989,
Pubmed
Mattaj,
Cap trimethylation of U snRNA is cytoplasmic and dependent on U snRNP protein binding.
1986,
Pubmed
,
Xenbase
Mattaj,
Nuclear segregation of U2 snRNA requires binding of specific snRNP proteins.
1985,
Pubmed
,
Xenbase
Newmeyer,
An N-ethylmaleimide-sensitive cytosolic factor necessary for nuclear protein import: requirement in signal-mediated binding to the nuclear pore.
1990,
Pubmed
,
Xenbase
Newmeyer,
Nuclear import can be separated into distinct steps in vitro: nuclear pore binding and translocation.
1988,
Pubmed
,
Xenbase
Newport,
The nucleus: structure, function, and dynamics.
1987,
Pubmed
Nigg,
Mechanisms of signal transduction to the cell nucleus.
1990,
Pubmed
Peters,
Fluorescence microphotolysis to measure nucleocytoplasmic transport and intracellular mobility.
1986,
Pubmed
Richardson,
Nuclear protein migration involves two steps: rapid binding at the nuclear envelope followed by slower translocation through nuclear pores.
1988,
Pubmed
,
Xenbase
Roberts,
The effect of protein context on nuclear location signal function.
1987,
Pubmed
Roth,
A gradient of nuclear localization of the dorsal protein determines dorsoventral pattern in the Drosophila embryo.
1989,
Pubmed
Rushlow,
The graded distribution of the dorsal morphogen is initiated by selective nuclear transport in Drosophila.
1989,
Pubmed
Sauterer,
Cytoplasmic assembly of snRNP particles from stored proteins and newly transcribed snRNA's in L929 mouse fibroblasts.
1988,
Pubmed
Silver,
Yeast proteins that recognize nuclear localization sequences.
1989,
Pubmed
,
Xenbase
Singh,
Gamma-monomethyl phosphate: a cap structure in spliceosomal U6 small nuclear RNA.
1989,
Pubmed
Steward,
Relocalization of the dorsal protein from the cytoplasm to the nucleus correlates with its function.
1989,
Pubmed
Taylor,
Xenopus myc proto-oncogene during development: expression as a stable maternal mRNA uncoupled from cell division.
1986,
Pubmed
,
Xenbase
Walter,
Signal recognition. Two receptors act sequentially.
,
Pubmed
White,
Nucleus-specific and temporally restricted localization of proteins in Tetrahymena macronuclei and micronuclei.
1989,
Pubmed
Yamasaki,
Identification of four nuclear transport signal-binding proteins that interact with diverse transport signals.
1989,
Pubmed
,
Xenbase
Zasloff,
tRNA transport from the nucleus in a eukaryotic cell: carrier-mediated translocation process.
1983,
Pubmed
,
Xenbase
Zieve,
Cell biology of the snRNP particles.
1990,
Pubmed
