Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Freeze-fracture and electrophysiological studies of newly developed acetylcholine receptors in Xenopus embryonic muscle cells.
Bridgman PC
,
Nakajima S
,
Greenberg AS
,
Nakajima Y
.
???displayArticle.abstract???
The development of acetylcholine receptors on Xenopus embryonic muscle cells both in culture and in situ was studied using electrophysiology and freeze-fracture electron microscopy. Acetylcholine sensitivity first appeared at developmental stage 20 and gradually increased up to about stage 31. Freeze-fracture of muscle cells that were nonsensitive to acetylcholine revealed diffusely distributed small P-face intramembraneous particles. When cells acquired sensitivity to acetylcholine, a different group of diffusely distributed large P-face particles began to appear. This group of particles was analyzed by subtracting the size distribution found on nonsensitive cells from that found on sensitive cells. We call this group of particles difference particles. The sizes of difference particles were large (peak diameter 11 nm). The density of difference particles gradually increased with development. The density of small particles (less than 9 nm) did not change with development. At later stages (32-36) aggregates of large particles appeared, which probably represent acetylcholine receptor clusters. The size distribution of difference particles was close to that of the aggregated particles, suggesting that at least part of difference particles represent diffusely distributed acetylcholine receptors. Difference particles exist mostly in solitary form (occasionally double), indicating that an acetylcholine receptor can be functional in solitary form. This result also shows that diffuse acetylcholine receptors that have previously been observed with 125I-alpha-bungarotoxin autoradiography do indeed exist in solitary forms not as microaggregates.
Anderson,
Nerve-induced and spontaneous redistribution of acetylcholine receptors on cultured muscle cells.
1977, Pubmed,
Xenbase
Anderson,
Nerve-induced and spontaneous redistribution of acetylcholine receptors on cultured muscle cells.
1977,
Pubmed
,
Xenbase
Anderson,
Effects of innervation on the distribution of acetylcholine receptors on cultured muscle cells.
1977,
Pubmed
,
Xenbase
Bevan,
The distribution of alpha-bungarotoxin binding sites of mammalian skeletal muscle developing in vivo.
1977,
Pubmed
Blackshaw,
Low resistance junctions between mesoderm cells during development of trunk muscles.
1976,
Pubmed
,
Xenbase
Blackshaw,
Onset of acetylcholine sensitivity and endplate activity in developing myotome muscles of Xenopus.
1976,
Pubmed
,
Xenbase
Bloch,
The localization of acetylcholine receptor clusters in areas of cell-substrate contact in cultures of rat myotubes.
1980,
Pubmed
Burden,
Development of the neuromuscular junction in the chick embryo: the number, distribution, and stability of acetylcholine receptors.
1977,
Pubmed
Cartaud,
Presence of a lattice structure in membrane fragments rich in nicotinic receptor protein from the electric organ of Torpedo marmorata.
1973,
Pubmed
Cohen,
Clusters of intramembrane particles associated with binding sites for alpha-bungarotoxin in cultured chick myotubes.
1979,
Pubmed
DIAMOND,
A study of foetal and new-born rat muscle fibres.
1962,
Pubmed
Dreyer,
Iontophoretic application of acetylcholine: advantages of high resistance micropipettes in connection with an electronic current pump.
1974,
Pubmed
Fambrough,
Development of acetylcholine sensitivity during myogenesis.
1971,
Pubmed
Fambrough,
Control of acetylcholine receptors in skeletal muscle.
1979,
Pubmed
Fischbach,
The distribution of acetylcholine sensitivity over uninnervated and innervated muscle fibers grown in cell culture.
1973,
Pubmed
Frank,
Early events in neuromuscular junction formation in vitro: induction of acetylcholine receptor clusters in the postsynaptic membrane and morphology of newly formed synapses.
1979,
Pubmed
Gruener,
Acetylcholine sensitivity of innervated and noninnervated Xenopus muscle cells in culture.
1982,
Pubmed
,
Xenbase
Hamilton,
The formation of somites in Xenopus.
1969,
Pubmed
,
Xenbase
Hartzell,
Acetycholine receptor production and incorporation into membranes of developing muscle fibers.
1973,
Pubmed
Heuser,
Functional changes in frog neuromuscular junctions studied with freeze-fracture.
1974,
Pubmed
Heuser,
Organization of acetylcholine receptors in quick-frozen, deep-etched, and rotary-replicated Torpedo postsynaptic membrane.
1979,
Pubmed
Hirokawa,
Internal and external differentiations of the postsynaptic membrane at the neuromuscular junction.
1982,
Pubmed
Kano,
Innervation and acetylcholine sensitivity of skeletal muscle cells differentiated in vitro from chick embryo.
1971,
Pubmed
Kidokoro,
Changes in synaptic potential properties during acetylcholine receptor accumulation and neurospecific interactions in Xenopus nerve-muscle cell culture.
1980,
Pubmed
,
Xenbase
Kidokoro,
Distribution and density of alpha-bungarotoxin binding sites on innervated and noninnervated Xenopus muscle cells in culture.
1982,
Pubmed
,
Xenbase
Kidokoro,
Synapse formation and neurotrophic effects on muscle cell lines.
1976,
Pubmed
Kuffler,
The distribution of acetylcholine sensitivity at the post-synaptic membrane of vertebrate skeletal twitch muscles: iontophoretic mapping in the micron range.
1975,
Pubmed
Kullberg,
Development of the myotomal neuromuscular junction in Xenopus laevis: an electrophysiological and fine-structural study.
1977,
Pubmed
,
Xenbase
Land,
Acetylcholine receptor distribution on myotubes in culture correlated to acetylcholine sensitivity.
1977,
Pubmed
Land,
Acetylcholine receptor site density affects the rising phase of miniature endplate currents.
1980,
Pubmed
Letinsky,
Acetylcholine sensitivity changes in tadpole tail muscle fibers innervated by developing motor neurons.
1975,
Pubmed
Lindstrom,
Structure and function of the acetylcholine receptor molecule studied using monoclonal antibodies.
1981,
Pubmed
Loring,
Denervation increases turnover rate of junctional acetylcholine receptors.
1980,
Pubmed
Moody-Corbett,
Localization of cholinesterase at sites of high acetylcholine receptor density on embryonic amphibian muscle cells cultured without nerve.
1981,
Pubmed
,
Xenbase
Nickel,
Ultrastructure of isolated membranes of Torpedo electric tissue.
1973,
Pubmed
Ohmori,
Development of neuromuscular transmission in a larval tunicate.
1977,
Pubmed
Peng,
Development of the postsynaptic membrane in Xenopus neuromuscular cultures observed by freeze-fracture and thin-section electron microscopy.
1980,
Pubmed
,
Xenbase
Peng,
Membrane particle aggregates in innervated and noninnervated cultures of Xenopus embryonic muscle cells.
1978,
Pubmed
,
Xenbase
Peper,
Structure and ultrastructure of the frog motor endplate. A freeze-etching study.
1974,
Pubmed
Pumplin,
Myasthenic patients' IgG causes redistribution of acetylcholine receptors: freeze-fracture studies.
1983,
Pubmed
Steinbach,
Nerve-muscle interaction in vitro. Role of acetylcholine.
1973,
Pubmed
Sytkowski,
Development of acetylcholine receptor clusters on cultured muscle cells.
1973,
Pubmed
Takahashi,
Slow and fast groups of pyramidal tract cells and their respective membrane properties.
1965,
Pubmed
Tao-Cheng,
Nodal and paranodal membrane structure in complementary freeze-fracture replicas of amphibian peripheral nerves.
1980,
Pubmed
Vogel,
Acetylcholine receptors of muscle grown in vitro.
1972,
Pubmed
Yee,
Clusters of intramembranous particles on cultured myotubes at sites that are highly sensitive to acetylcholine.
1978,
Pubmed
