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Mol Cell Biol
1998 May 01;185:2867-75. doi: 10.1128/MCB.18.5.2867.
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Axil, a member of the Axin family, interacts with both glycogen synthase kinase 3beta and beta-catenin and inhibits axis formation of Xenopus embryos.
Yamamoto H
,
Kishida S
,
Uochi T
,
Ikeda S
,
Koyama S
,
Asashima M
,
Kikuchi A
.
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Using a yeast two-hybrid method, we identified a novel protein which interacts with glycogen synthase kinase 3beta (GSK-3beta). This protein had 44% amino acid identity with Axin, a negative regulator of the Wnt signaling pathway. We designated this protein Axil for Axin like. Like Axin, Axil ventralized Xenopus embryos and inhibited Xwnt8-induced Xenopus axis duplication. Axil was phosphorylated by GSK-3beta. Axil bound not only to GSK-3beta but also to beta-catenin, and the GSK-3beta-binding site of Axil was distinct from the beta-catenin-binding site. Furthermore, Axil enhanced GSK-3beta-dependent phosphorylation of beta-catenin. These results indicate that Axil negatively regulates the Wnt signaling pathway by mediating GSK-3beta-dependent phosphorylation of beta-catenin, thereby inhibiting axis formation.
Aberle,
beta-catenin is a target for the ubiquitin-proteasome pathway.
1997, Pubmed
Aberle,
beta-catenin is a target for the ubiquitin-proteasome pathway.
1997,
Pubmed
Behrens,
Functional interaction of beta-catenin with the transcription factor LEF-1.
1996,
Pubmed
,
Xenbase
Bhanot,
A new member of the frizzled family from Drosophila functions as a Wingless receptor.
1996,
Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Cadigan,
Wnt meeting 1996.
1997,
Pubmed
,
Xenbase
Cook,
Wingless inactivates glycogen synthase kinase-3 via an intracellular signalling pathway which involves a protein kinase C.
1996,
Pubmed
Dohlman,
RGS proteins and signaling by heterotrimeric G proteins.
1997,
Pubmed
Druey,
Inhibition of G-protein-mediated MAP kinase activation by a new mammalian gene family.
1996,
Pubmed
GLUECKSOHN-SCHOENHEIMER,
The effects of a lethal mutation responsible for duplications and twinning in mouse embryos.
1949,
Pubmed
He,
Glycogen synthase kinase-3 and dorsoventral patterning in Xenopus embryos.
1995,
Pubmed
,
Xenbase
Hinoi,
Post-translational modifications of Ras and Ral are important for the action of Ral GDP dissociation stimulator.
1996,
Pubmed
Ikeda,
Identification and characterization of a novel protein interacting with Ral-binding protein 1, a putative effector protein of Ral.
1998,
Pubmed
Ikeda,
Axin, a negative regulator of the Wnt signaling pathway, forms a complex with GSK-3beta and beta-catenin and promotes GSK-3beta-dependent phosphorylation of beta-catenin.
1998,
Pubmed
Jacobs-Cohen,
Knobbly, a new dominant mutation in the mouse that affects embryonic ectoderm organization.
1984,
Pubmed
Kao,
The entire mesodermal mantle behaves as Spemann's organizer in dorsoanterior enhanced Xenopus laevis embryos.
1988,
Pubmed
,
Xenbase
Kikuchi,
Regulation of interaction of ras p21 with RalGDS and Raf-1 by cyclic AMP-dependent protein kinase.
1996,
Pubmed
Kishida,
Axin, a negative regulator of the wnt signaling pathway, directly interacts with adenomatous polyposis coli and regulates the stabilization of beta-catenin.
1998,
Pubmed
Korinek,
Constitutive transcriptional activation by a beta-catenin-Tcf complex in APC-/- colon carcinoma.
1997,
Pubmed
Kozak,
An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs.
1987,
Pubmed
Krieg,
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
1984,
Pubmed
,
Xenbase
Miller,
Signal transduction through beta-catenin and specification of cell fate during embryogenesis.
1996,
Pubmed
Mizushima,
pEF-BOS, a powerful mammalian expression vector.
1990,
Pubmed
Molenaar,
XTcf-3 transcription factor mediates beta-catenin-induced axis formation in Xenopus embryos.
1996,
Pubmed
,
Xenbase
Morin,
Activation of beta-catenin-Tcf signaling in colon cancer by mutations in beta-catenin or APC.
1997,
Pubmed
Munemitsu,
Regulation of intracellular beta-catenin levels by the adenomatous polyposis coli (APC) tumor-suppressor protein.
1995,
Pubmed
Munemitsu,
Deletion of an amino-terminal sequence beta-catenin in vivo and promotes hyperphosporylation of the adenomatous polyposis coli tumor suppressor protein.
1996,
Pubmed
Murai,
Tyrosine dephosphorylation of glycogen synthase kinase-3 is involved in its extracellular signal-dependent inactivation.
1996,
Pubmed
Murai,
Characterization of Ral GDP dissociation stimulator-like (RGL) activities to regulate c-fos promoter and the GDP/GTP exchange of Ral.
1997,
Pubmed
Nusse,
Wnt genes.
1992,
Pubmed
Nusse,
A versatile transcriptional effector of Wingless signaling.
1997,
Pubmed
Peifer,
Beta-catenin as oncogene: the smoking gun.
1997,
Pubmed
Perry,
Phenotypic and molecular analysis of a transgenic insertional allele of the mouse Fused locus.
1995,
Pubmed
Plyte,
Glycogen synthase kinase-3: functions in oncogenesis and development.
1992,
Pubmed
Polakis,
The adenomatous polyposis coli (APC) tumor suppressor.
1997,
Pubmed
Reed,
The Inheritance and Expression of Fused, a New Mutation in the House Mouse.
1937,
Pubmed
Rubinfeld,
Stabilization of beta-catenin by genetic defects in melanoma cell lines.
1997,
Pubmed
Rubinfeld,
Binding of GSK3beta to the APC-beta-catenin complex and regulation of complex assembly.
1996,
Pubmed
Slusarski,
Interaction of Wnt and a Frizzled homologue triggers G-protein-linked phosphatidylinositol signalling.
1997,
Pubmed
,
Xenbase
Sokol,
Injected Wnt RNA induces a complete body axis in Xenopus embryos.
1991,
Pubmed
,
Xenbase
Sussman,
Isolation and characterization of a mouse homolog of the Drosophila segment polarity gene dishevelled.
1994,
Pubmed
Vojtek,
Mammalian Ras interacts directly with the serine/threonine kinase Raf.
1993,
Pubmed
Wang,
Glycogen synthase kinase-3 beta is a dual specificity kinase differentially regulated by tyrosine and serine/threonine phosphorylation.
1994,
Pubmed
Yost,
The axis-inducing activity, stability, and subcellular distribution of beta-catenin is regulated in Xenopus embryos by glycogen synthase kinase 3.
1996,
Pubmed
,
Xenbase
Zeng,
The mouse Fused locus encodes Axin, an inhibitor of the Wnt signaling pathway that regulates embryonic axis formation.
1997,
Pubmed
,
Xenbase
