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EMBO J
1992 Mar 01;113:885-90. doi: 10.1002/j.1460-2075.1992.tb05126.x.
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Calcium channel beta subunit heterogeneity: functional expression of cloned cDNA from heart, aorta and brain.
Hullin R
,
Singer-Lahat D
,
Freichel M
,
Biel M
,
Dascal N
,
Hofmann F
,
Flockerzi V
.
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Complementary DNAs encoding three novel and distinct beta subunits (CaB2a, CaB2b and CaB3) of the high voltage activated (L-type) calcium channel have been isolated from rabbit heart. Their deduced amino acid sequence is homologous to the beta subunit originally cloned from skeletal muscle (CaB1). CaB2a and CaB2b are splicing products of a common primary transcript (CaB2). Northern analysis and specific amplification of CaB2 and CaB3 specific cDNAs by polymerase chain reactions showed that CaB2 is predominantly expressed in heart, aorta and brain, whereas CaB3 is most abundant in brain but also present in aorta, trachea, lung, heart and skeletal muscle. A partial DNA sequence complementary to a third variant of the CaB2 gene, subtype CaB2c, has also been cloned from rabbit brain. Coexpression of CaB2a, CaB2b and CaB3 with alpha 1heart enhances not only the expression in the oocyte of the channel directed by the cardiac alpha 1 subunit alone, but also effects its macroscopic characteristics such as drug sensitivity and kinetics. These results together with the known alpha 1 subunit heterogeneity, suggest that different types of calcium currents may depend on channel subunit composition.
Biel,
Primary structure and functional expression of a high voltage activated calcium channel from rabbit lung.
1990, Pubmed,
Xenbase
Biel,
Primary structure and functional expression of a high voltage activated calcium channel from rabbit lung.
1990,
Pubmed
,
Xenbase
Biel,
Tissue-specific expression of high-voltage-activated dihydropyridine-sensitive L-type calcium channels.
1991,
Pubmed
Bosse,
The cDNA and deduced amino acid sequence of the gamma subunit of the L-type calcium channel from rabbit skeletal muscle.
1990,
Pubmed
Brown,
Ionic channels and their regulation by G protein subunits.
1990,
Pubmed
Catterall,
Excitation-contraction coupling in vertebrate skeletal muscle: a tale of two calcium channels.
1991,
Pubmed
Chou,
Empirical predictions of protein conformation.
1978,
Pubmed
Curtis,
Phosphorylation of the calcium antagonist receptor of the voltage-sensitive calcium channel by cAMP-dependent protein kinase.
1985,
Pubmed
De Jongh,
Subunits of purified calcium channels: a 212-kDa form of alpha 1 and partial amino acid sequence of a phosphorylation site of an independent beta subunit.
1989,
Pubmed
Ellis,
Sequence and expression of mRNAs encoding the alpha 1 and alpha 2 subunits of a DHP-sensitive calcium channel.
1988,
Pubmed
Flockerzi,
Purification of a functional receptor for calcium-channel blockers from rabbit skeletal-muscle microsomes.
1986,
Pubmed
Hartzell,
Sympathetic regulation of cardiac calcium current is due exclusively to cAMP-dependent phosphorylation.
1991,
Pubmed
Hofmann,
The molecular structure and regulation of muscular calcium channels.
1990,
Pubmed
Jahn,
Site-specific phosphorylation of the purified receptor for calcium-channel blockers by cAMP- and cGMP-dependent protein kinases, protein kinase C, calmodulin-dependent protein kinase II and casein kinase II.
1988,
Pubmed
Jay,
Primary structure of the gamma subunit of the DHP-sensitive calcium channel from skeletal muscle.
1990,
Pubmed
Kameyama,
On the mechanism of beta-adrenergic regulation of the Ca channel in the guinea-pig heart.
1985,
Pubmed
Kennelly,
Consensus sequences as substrate specificity determinants for protein kinases and protein phosphatases.
1991,
Pubmed
Koch,
cDNA cloning of a dihydropyridine-sensitive calcium channel from rat aorta. Evidence for the existence of alternatively spliced forms.
1990,
Pubmed
Kozak,
An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs.
1987,
Pubmed
Kyte,
A simple method for displaying the hydropathic character of a protein.
1982,
Pubmed
Lacerda,
Normalization of current kinetics by interaction between the alpha 1 and beta subunits of the skeletal muscle dihydropyridine-sensitive Ca2+ channel.
1991,
Pubmed
Mikami,
Primary structure and functional expression of the cardiac dihydropyridine-sensitive calcium channel.
1989,
Pubmed
,
Xenbase
Mori,
Primary structure and functional expression from complementary DNA of a brain calcium channel.
1991,
Pubmed
,
Xenbase
Ruth,
Primary structure of the beta subunit of the DHP-sensitive calcium channel from skeletal muscle.
1989,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Singer,
The roles of the subunits in the function of the calcium channel.
1991,
Pubmed
,
Xenbase
Tanabe,
Primary structure of the receptor for calcium channel blockers from skeletal muscle.
,
Pubmed
Trautwein,
Regulation of cardiac L-type calcium current by phosphorylation and G proteins.
1990,
Pubmed
Tsien,
Molecular diversity of voltage-dependent Ca2+ channels.
1991,
Pubmed
Varadi,
Acceleration of activation and inactivation by the beta subunit of the skeletal muscle calcium channel.
1991,
Pubmed
